Recombinant Mouse R-Spondin 1 Protein, CF

A New rmR-Spondin 1 is Available! CHO expressed; ~5 fold better activity; Enhanced purity!
New Product 7150-RS/CF
  (31 citations)
(4 Reviews)
    
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Product Details
Citations (31)
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  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
  • Source
    E. coli-derived mouse R-Spondin 1 protein
    Ser21-Gly209, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    21 kDa
Product Datasheets

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3474-RS
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
 
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
 
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
 
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
 
Data Images
Recombinant Mouse R-Spondin 1 (Catalog # 3474-RS) induces activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is 50‑200 ng/mL in the presence of 5 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
1 μg/lane of Recombinant Mouse R-Spondin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 24 kDa.
Background: R-Spondin 1

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that belongs to the R-Spondin family (1, 2). R-Spondins share around 40% aa identity. All regulate Wnt/ beta -catenin signaling, but have distinct expression patterns (1-3). Like other R-spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (amino acids (aa) 34-135) followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta -catenin stabilization (2, 4). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (5). R-Spondin 1 contains one potential N-glycosylation site. Over aa 21 - 209, mouse R-Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 5). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (6, 7). Disruption is also associated with palmoplantar keratosis (6, 7). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (8). Injection of recombinant R-Spondin 1 in mice causes activation of beta -catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (8, 9). R-Spondin 1 appears to regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptor, Kremen (10). This competition reduces internalization of DKK-1/LRP-6/Kremen complexes (10). Reports differ on whether
R-Spondin 1 binds LRP-6 directly (10 - 12).

  • References:
    1. Lowther, W. et al. (2005) J. Virol. 79:10093.
    2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
    3. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
    4. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
    5. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
    6. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
    7. Parma, P. et al. (2006) Nat. Genet. 38:1304.
    8. Kim, K.-A. et al. (2005) Science 309:1256.
    9. Zhao, J. et al. (2007) Gastroenterology 132:1331.
    10. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
    11. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
    12. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
  • Long Name:
    Roof Plate-specific Spondin 1
  • Entrez Gene IDs:
    284654 (Human); 192199 (Mouse)
  • Alternate Names:
    Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; hRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

31 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Characterization of the enhancer and promoter landscape of inflammatory bowel disease from human colon biopsies
    Authors: M Boyd, M Thodberg, M Vitezic, J Bornholdt, K Vitting-Se, Y Chen, M Coskun, Y Li, BZS Lo, P Klausen, P Jan Schwei, AG Pedersen, N Rapin, K Skovgaard, K Dahlgaard, R Andersson, TB Terkelsen, B Lilje, JT Troelsen, AM Petersen, KB Jensen, I Gögenur, P Thielsen, JB Seidelin, OH Nielsen, JT Bjerrum, A Sandelin
    Nat Commun, 2018;9(1):1661.
    Species: Human
    Sample Type: Organoids
    Application: Bioassay
  2. The Coordinated Activities of nAChR and Wnt Signaling Regulate Intestinal Stem Cell Function in Mice
    Authors: T Takahashi, A Shiraishi, J Murata
    Int J Mol Sci, 2018;19(3):.
    Species: Mouse
    Sample Type: Complex Sample Type
    Application: Bioassay
  3. YAP/TAZ-Dependent Reprogramming of Colonic Epithelium Links ECM Remodeling to Tissue Regeneration
    Authors: S Yui, L Azzolin, M Maimets, MT Pedersen, RP Fordham, SL Hansen, HL Larsen, J Guiu, MRP Alves, CF Rundsten, JV Johansen, Y Li, CD Madsen, T Nakamura, M Watanabe, OH Nielsen, PJ Schweiger, S Piccolo, KB Jensen
    Cell Stem Cell, 2018;22(1):35-49.e7.
    Species: Mouse
    Sample Type: Complex Sample Type
    Application: Bioassay
  4. Aging effects on intestinal homeostasis associated with expansion and dysfunction of intestinal epithelial stem cells
    Authors: EC Moorefield, SF Andres, RE Blue, L Van Landeg, AT Mah, MA Santoro, S Ding
    Aging (Albany NY), 2017;9(8):1898-1915.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  5. Organotypic pancreatoids with native mesenchyme develop Insulin producing endocrine cells
    Authors: MA Scavuzzo, D Yang, M Borowiak
    Sci Rep, 2017;7(1):10810.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  6. In Vitro Polarization of Colonoids to Create an Intestinal Stem Cell Compartment
    Authors: PJ Attayek, AA Ahmad, Y Wang, I Williamson, CE Sims, ST Magness, NL Allbritton
    PLoS ONE, 2016;11(4):e0153795.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  7. Directed Differentiation of Human Embryonic Stem Cells into Prostate Organoids In Vitro and its Perturbation by Low-Dose Bisphenol A Exposure.
    Authors: Calderon-Gierszal E, Prins G
    PLoS ONE, 2015;10(7):e0133238.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  8. Bmi1 regulates murine intestinal stem cell proliferation and self-renewal downstream of Notch.
    Authors: Lopez-Arribillaga E, Rodilla V, Pellegrinet L, Guiu J, Iglesias M, Roman A, Gutarra S, Gonzalez S, Munoz-Canoves P, Fernandez-Salguero P, Radtke F, Bigas A, Espinosa L
    Development, 2015;142(1):41-50.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  9. Compartmentalized accumulation of cAMP near complexes of multidrug resistance protein 4 (MRP4) and cystic fibrosis transmembrane conductance regulator (CFTR) contributes to drug-induced diarrhea.
    Authors: Moon C, Zhang W, Ren A, Arora K, Sinha C, Yarlagadda S, Woodrooffe K, Schuetz J, Valasani K, de Jonge H, Shanmukhappa S, Shata M, Buddington R, Parthasarathi K, Naren A
    J Biol Chem, 2015;290(18):11246-57.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  10. Inducible in vivo genome editing with CRISPR-Cas9.
    Authors: Dow, Lukas E, Fisher, Jonathan, O'Rourke, Kevin P, Muley, Ashlesha, Kastenhuber, Edward R, Livshits, Geulah, Tschaharganeh, Darjus F, Socci, Nicholas, Lowe, Scott W
    Nat Biotechnol, 2015;33(4):390-4.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Bioassay
  11. Selenoprotein P influences colitis-induced tumorigenesis by mediating stemness and oxidative damage.
    Authors: Barrett C, Reddy V, Short S, Motley A, Lintel M, Bradley A, Freeman T, Vallance J, Ning W, Parang B, Poindexter S, Fingleton B, Chen X, Washington M, Wilson K, Shroyer N, Hill K, Burk R, Williams C
    J Clin Invest, 2015;125(7):2646-60.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  12. Convergence of cMyc and beta-catenin on Tcf7l1 enables endoderm specification.
    Authors: Morrison G, Scognamiglio R, Trumpp A, Smith A
    EMBO J, 2015;35(3):356-68.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  13. R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis.
    Authors: Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, Catizone A, Ricci G
    PLoS ONE, 2015;10(4):e0124213.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  14. Proinflammatory cytokine-induced tight junction remodeling through dynamic self-assembly of claudins.
    Authors: Capaldo C, Farkas A, Hilgarth R, Krug S, Wolf M, Benedik J, Fromm M, Koval M, Parkos C, Nusrat A
    Mol Biol Cell, 2014;25(18):2710-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  15. Blockade of TLR3 protects mice from lethal radiation-induced gastrointestinal syndrome.
    Authors: Takemura N, Kawasaki T, Kunisawa J, Sato S, Lamichhane A, Kobiyama K, Aoshi T, Ito J, Mizuguchi K, Karuppuchamy T, Matsunaga K, Miyatake S, Mori N, Tsujimura T, Satoh T, Kumagai Y, Kawai T, Standley D, Ishii K, Kiyono H, Akira S, Uematsu S
    Nat Commun, 2014;5(0):3492.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  16. Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta.
    Authors: Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K
    Proc Natl Acad Sci U S A, 2014;111(21):E2229-36.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  17. In vitro multilineage differentiation and self-renewal of single pancreatic colony-forming cells from adult C57BL/6 mice.
    Authors: Jin, Liang, Feng, Tao, Zerda, Ricardo, Chen, Ching-Ch, Riggs, Arthur D, Ku, Hsun Ter
    Stem Cells Dev, 2014;23(8):899-909.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  18. A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures.
    Authors: Matsumoto, Shinji, Fujii, Shinsuke, Sato, Akira, Ibuka, Souji, Kagawa, Yoshinor, Ishii, Masaru, Kikuchi, Akira
    EMBO J, 2014;33(7):702-18.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  19. An enteroendocrine cell-enteric glia connection revealed by 3D electron microscopy.
    Authors: Bohorquez D, Samsa L, Roholt A, Medicetty S, Chandra R, Liddle R
    PLoS ONE, 2014;9(2):e89881.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  20. Chronic inflammation induces telomere dysfunction and accelerates ageing in mice.
    Authors: Jurk D, Wilson C, Passos J, Oakley F, Correia-Melo C, Greaves L, Saretzki G, Fox C, Lawless C, Anderson R, Hewitt G, Pender S, Fullard N, Nelson G, Mann J, van de Sluis B, Mann D, von Zglinicki T
    Nat Commun, 2014;2(0):4172.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  21. beta-Catenin is required for hair-cell differentiation in the cochlea.
    Authors: Shi F, Hu L, Jacques B, Mulvaney J, Dabdoub A, Edge A
    J Neurosci, 2014;34(19):6470-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  22. Impact of diet-induced obesity on intestinal stem cells: hyperproliferation but impaired intrinsic function that requires insulin/IGF1.
    Authors: Mah, Amanda T, Van Landeghem, Lauriann, Gavin, Hannah E, Magness, Scott T, Lund, P Kay
    Endocrinology, 2014;155(9):3302-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  23. LGR4 and its ligands, R-spondin 1 and R-spondin 3, regulate food intake in the hypothalamus of male rats.
    Authors: Li, Ji-Yao, Chai, Biaoxin, Zhang, Weizhen, Fritze, Danielle, Zhang, Chao, Mulholland, Michael
    Endocrinology, 2014;155(2):429-40.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  24. The ErbB4 ligand neuregulin-4 protects against experimental necrotizing enterocolitis.
    Authors: McElroy S, Castle S, Bernard J, Almohazey D, Hunter C, Bell B, Al Alam D, Wang L, Ford H, Frey M
    Am J Pathol, 2014;184(10):2768-78.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  25. Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium.
    Authors: Skoczek D, Walczysko P, Horn N, Parris A, Clare S, Williams M, Sobolewski A
    J Immunol, 2014;193(1):439-51.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  26. Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon.
    Authors: Fukuda M, Mizutani T, Mochizuki W, Matsumoto T, Nozaki K, Sakamaki Y, Ichinose S, Okada Y, Tanaka T, Watanabe M, Nakamura T
    Genes Dev, 2014;28(16):1752-7.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  27. Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel.
    Authors: Jin L, Feng T, Shih H, Zerda R, Luo A, Hsu J, Mahdavi A, Sander M, Tirrell D, Riggs A, Ku H
    Proc Natl Acad Sci U S A, 2013;110(10):3907-12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  28. In vivo and in vitro models for the therapeutic targeting of Wnt signaling using a Tet-ODeltaN89beta-catenin system.
    Authors: Jarde T, Evans R, McQuillan K, Parry L, Feng G, Alvares B, Clarke A, Dale T
    Oncogene, 2013;32(7):883-93.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  29. CSF-1 receptor-dependent colon development, homeostasis and inflammatory stress response.
    Authors: Huynh D, Akcora D, Malaterre J, Chan C, Dai X, Bertoncello I, Stanley E, Ramsay R
    PLoS ONE, 2013;8(2):e56951.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  30. Lrig1 controls intestinal stem-cell homeostasis by negative regulation of ErbB signalling.
    Authors: Wong, Vivian W, Stange, Daniel E, Page, Mahalia, Buczacki, Simon, Wabik, Agnieszk, Itami, Satoshi, van de Wetering, Marc, Poulsom, Richard, Wright, Nicholas, Trotter, Matthew, Watt, Fiona M, Winton, Doug J, Clevers, Hans, Jensen, Kim B
    Nat Cell Biol, 2012;14(4):401-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: cell culture
  31. Human RSPO1/R-spondin1 is expressed during early ovary development and augments beta-catenin signaling.
    Authors: Tomaselli S, Megiorni F, Lin L, Mazzilli MC, Gerrelli D, Majore S, Grammatico P, Achermann JC
    PLoS ONE, 2011;6(1):e16366.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
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Recombinant Mouse Wnt-3a Protein, CF

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Reconstitution Buffer 1 (PBS)

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Average Rating: 4.8 (Based on 4 reviews)

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 Cell culture Anonymous 09/08/2017
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 Apoptosis assay Anonymous 09/08/2017
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Application Apoptosis assay
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 Stem/Immune cell maintenance or differentiation Anonymous 12/15/2016
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Application Stem/Immune cell maintenance or differentiation

Other Experimental Details

Other Experimental Details Used in culture media to differentiate stem cells towards a pancreatic endocrine progenitor cell fate.

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