Recombinant Mouse R-Spondin 1 Protein, CF 3474-RS-050: R&D Systems

Recombinant Mouse R-Spondin 1 Protein, CF

A New rmR-Spondin 1 is Available! CHO expressed; ~5 fold better activity; Enhanced purity!
New Product 7150-RS/CF.
(26 citations)
(4 Reviews)
  
  • Purity
    >90%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The typical ED50 is 50-200 ng/mL in the presence of 5 ng/mL recombinant mouse Wnt-3a.
  • Source
    E. coli-derived Ser21-Gly209, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    21 kDa
3474-RS
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 250 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse R-Spondin 1 (Catalog # 3474-RS) induces activation of beta -catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is 50‑200 ng/mL in the presence of 5 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
1 μg/lane of Recombinant Mouse R-Spondin-1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 24 kDa.
Background: R-Spondin 1

R-Spondin 1 (RSPO1, Roof plate-specific Spondin 1), also known as cysteine-rich and single thrombospondin domain containing protein 3 (Cristin 3), is a 27 kDa secreted protein that belongs to the R-Spondin family (1, 2). R-Spondins share around 40% aa identity. All regulate Wnt/ beta -catenin signaling, but have distinct expression patterns (1-3). Like other R-spondins, R-Spondin 1 contains two adjacent cysteine-rich furin-like domains (amino acids (aa) 34-135) followed by a thrombospondin (TSP-1) motif (aa 147-207) and a region rich in basic residues (aa 211-263). Only the furin-like domains are needed for beta -catenin stabilization (2, 4). A putative nuclear localization signal at the C-terminus may allow some expression in the nucleus (5). R-Spondin 1 contains one potential N-glycosylation site. Over aa 21 - 209, mouse R-Spondin 1 shares 98%, 94%, 94%, 93%, 92% and 88% aa identity with rat, human, horse, cow, goat and dog RSPO-1, respectively. R-Spondin 1 is expressed in early development at the roof plate boundary and is thought to contribute to dorsal neural tube development (3, 5). In humans, rare disruptions of the R-Spondin 1 gene are associated with tendencies for XX sex reversal (phenotypic male) or hermaphroditism, indicating a role for R-Spondin 1 in gender-specific differentiation (6, 7). Disruption is also associated with palmoplantar keratosis (6, 7). Postnatally, R-Spondin 1 is expressed by neuroendocrine cells in the intestine, adrenal gland and pancreas, and by epithelia in kidney and prostate (8). Injection of recombinant R-Spondin 1 in mice causes activation of beta -catenin and proliferation of intestinal crypt epithelial cells, and ameliorates experimental colitis (8, 9). R-Spondin 1 appears to regulate Wnt/ beta -catenin by competing with the Wnt antagonist DKK-1 for binding to the Wnt co-receptor, Kremen (10). This competition reduces internalization of DKK-1/LRP-6/Kremen complexes (10). Reports differ on whether
R-Spondin 1 binds LRP-6 directly (10 - 12).

  • References:
    1. Lowther, W. et al. (2005) J. Virol. 79:10093.
    2. Kim, K.-A. et al. (2006) Cell Cycle 5:23.
    3. Nam, J.-S. et al. (2007) Gene Expr. Patterns 7:306.
    4. Kazanskaya, O. et al. (2004) Dev. Cell 7:525.
    5. Kamata, T. et al. (2004) Biochim. Biophys. Acta 1676:51.
    6. Tomaselli, S. et al. (2008) Hum. Mutat. 29:220.
    7. Parma, P. et al. (2006) Nat. Genet. 38:1304.
    8. Kim, K.-A. et al. (2005) Science 309:1256.
    9. Zhao, J. et al. (2007) Gastroenterology 132:1331.
    10. Binnerts, M.E. et al. (2007) Proc. Natl. Acad. Sci. USA 104:14700.
    11. Nam, J.-S. et al. (2006) J. Biol. Chem. 281:13247.
    12. Wei, Q. et al. (2007) J. Biol. Chem. 282:15903.
  • Long Name:
    Roof Plate-specific Spondin 1
  • Entrez Gene IDs:
    284654 (Human); 192199 (Mouse)
  • Alternate Names:
    Cristin 3; CRISTIN3; FLJ40906Roof plate-specific spondin-1; hRspo1; roof plate-specific spondin; RSPO; RSPO1; RSpondin 1; R-Spondin 1; R-spondin homolog (Xenopus laevis); RSPONDIN; R-spondin1; R-spondin-1
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

26 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. In Vitro Polarization of Colonoids to Create an Intestinal Stem Cell Compartment
    Authors: PJ Attayek, AA Ahmad, Y Wang, I Williamson, CE Sims, ST Magness, NL Allbritton
    PLoS ONE, 2016;11(4):e0153795.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  2. Selenoprotein P influences colitis-induced tumorigenesis by mediating stemness and oxidative damage.
    Authors: Barrett C, Reddy V, Short S, Motley A, Lintel M, Bradley A, Freeman T, Vallance J, Ning W, Parang B, Poindexter S, Fingleton B, Chen X, Washington M, Wilson K, Shroyer N, Hill K, Burk R, Williams C
    J Clin Invest, 2015;125(7):2646-60.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  3. Convergence of cMyc and beta-catenin on Tcf7l1 enables endoderm specification.
    Authors: Morrison G, Scognamiglio R, Trumpp A, Smith A
    EMBO J, 2015;35(3):356-68.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  4. Directed Differentiation of Human Embryonic Stem Cells into Prostate Organoids In Vitro and its Perturbation by Low-Dose Bisphenol A Exposure.
    Authors: Calderon-Gierszal E, Prins G
    PLoS ONE, 2015;10(7):e0133238.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
  5. Inducible in vivo genome editing with CRISPR-Cas9.
    Authors: Dow, Lukas E, Fisher, Jonathan, O'Rourke, Kevin P, Muley, Ashlesha, Kastenhuber, Edward R, Livshits, Geulah, Tschaharganeh, Darjus F, Socci, Nicholas, Lowe, Scott W
    Nat Biotechnol, 2015;33(4):390-4.
    Species: Mouse
    Sample Type: Tissue Homogenates
    Application: Bioassay
  6. Bmi1 regulates murine intestinal stem cell proliferation and self-renewal downstream of Notch.
    Authors: Lopez-Arribillaga E, Rodilla V, Pellegrinet L, Guiu J, Iglesias M, Roman A, Gutarra S, Gonzalez S, Munoz-Canoves P, Fernandez-Salguero P, Radtke F, Bigas A, Espinosa L
    Development, 2015;142(1):41-50.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  7. R-spondin 1/dickkopf-1/beta-catenin machinery is involved in testicular embryonic angiogenesis.
    Authors: Caruso M, Ferranti F, Corano Scheri K, Dobrowolny G, Ciccarone F, Grammatico P, Catizone A, Ricci G
    PLoS ONE, 2015;10(4):e0124213.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  8. Compartmentalized accumulation of cAMP near complexes of multidrug resistance protein 4 (MRP4) and cystic fibrosis transmembrane conductance regulator (CFTR) contributes to drug-induced diarrhea.
    Authors: Moon C, Zhang W, Ren A, Arora K, Sinha C, Yarlagadda S, Woodrooffe K, Schuetz J, Valasani K, de Jonge H, Shanmukhappa S, Shata M, Buddington R, Parthasarathi K, Naren A
    J Biol Chem, 2015;290(18):11246-57.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  9. LGR4 and its ligands, R-spondin 1 and R-spondin 3, regulate food intake in the hypothalamus of male rats.
    Authors: Li, Ji-Yao, Chai, Biaoxin, Zhang, Weizhen, Fritze, Danielle, Zhang, Chao, Mulholland, Michael
    Endocrinology, 2014;155(2):429-40.
    Species: Mouse
    Sample Type: In Vivo
    Application: In Vivo
  10. Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon.
    Authors: Fukuda M, Mizutani T, Mochizuki W, Matsumoto T, Nozaki K, Sakamaki Y, Ichinose S, Okada Y, Tanaka T, Watanabe M, Nakamura T
    Genes Dev, 2014;28(16):1752-7.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  11. Impact of diet-induced obesity on intestinal stem cells: hyperproliferation but impaired intrinsic function that requires insulin/IGF1.
    Authors: Mah, Amanda T, Van Landeghem, Lauriann, Gavin, Hannah E, Magness, Scott T, Lund, P Kay
    Endocrinology, 2014;155(9):3302-14.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  12. A combination of Wnt and growth factor signaling induces Arl4c expression to form epithelial tubular structures.
    Authors: Matsumoto, Shinji, Fujii, Shinsuke, Sato, Akira, Ibuka, Souji, Kagawa, Yoshinor, Ishii, Masaru, Kikuchi, Akira
    EMBO J, 2014;33(7):702-18.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  13. An enteroendocrine cell-enteric glia connection revealed by 3D electron microscopy.
    Authors: Bohorquez D, Samsa L, Roholt A, Medicetty S, Chandra R, Liddle R
    PLoS ONE, 2014;9(2):e89881.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  14. beta-Catenin is required for hair-cell differentiation in the cochlea.
    Authors: Shi F, Hu L, Jacques B, Mulvaney J, Dabdoub A, Edge A
    J Neurosci, 2014;34(19):6470-9.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  15. In vitro multilineage differentiation and self-renewal of single pancreatic colony-forming cells from adult C57BL/6 mice.
    Authors: Jin, Liang, Feng, Tao, Zerda, Ricardo, Chen, Ching-Ch, Riggs, Arthur D, Ku, Hsun Ter
    Stem Cells Dev, 2014;23(8):899-909.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  16. Blockade of TLR3 protects mice from lethal radiation-induced gastrointestinal syndrome.
    Authors: Takemura N, Kawasaki T, Kunisawa J, Sato S, Lamichhane A, Kobiyama K, Aoshi T, Ito J, Mizuguchi K, Karuppuchamy T, Matsunaga K, Miyatake S, Mori N, Tsujimura T, Satoh T, Kumagai Y, Kawai T, Standley D, Ishii K, Kiyono H, Akira S, Uematsu S
    Nat Commun, 2014;5(0):3492.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  17. Luminal microbes promote monocyte-stem cell interactions across a healthy colonic epithelium.
    Authors: Skoczek D, Walczysko P, Horn N, Parris A, Clare S, Williams M, Sobolewski A
    J Immunol, 2014;193(1):439-51.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  18. Proinflammatory cytokine-induced tight junction remodeling through dynamic self-assembly of claudins.
    Authors: Capaldo C, Farkas A, Hilgarth R, Krug S, Wolf M, Benedik J, Fromm M, Koval M, Parkos C, Nusrat A
    Mol Biol Cell, 2014;25(18):2710-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  19. Oncogenic mutations in intestinal adenomas regulate Bim-mediated apoptosis induced by TGF-beta.
    Authors: Wiener Z, Band A, Kallio P, Hogstrom J, Hyvonen V, Kaijalainen S, Ritvos O, Haglund C, Kruuna O, Robine S, Louvard D, Ben-Neriah Y, Alitalo K
    Proc Natl Acad Sci U S A, 2014;111(21):E2229-36.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  20. The ErbB4 ligand neuregulin-4 protects against experimental necrotizing enterocolitis.
    Authors: McElroy S, Castle S, Bernard J, Almohazey D, Hunter C, Bell B, Al Alam D, Wang L, Ford H, Frey M
    Am J Pathol, 2014;184(10):2768-78.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  21. Chronic inflammation induces telomere dysfunction and accelerates ageing in mice.
    Authors: Jurk D, Wilson C, Passos J, Oakley F, Correia-Melo C, Greaves L, Saretzki G, Fox C, Lawless C, Anderson R, Hewitt G, Pender S, Fullard N, Nelson G, Mann J, van de Sluis B, Mann D, von Zglinicki T
    Nat Commun, 2014;2(0):4172.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  22. In vivo and in vitro models for the therapeutic targeting of Wnt signaling using a Tet-ODeltaN89beta-catenin system.
    Authors: Jarde T, Evans R, McQuillan K, Parry L, Feng G, Alvares B, Clarke A, Dale T
    Oncogene, 2013;32(7):883-93.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: Bioassay
  23. CSF-1 receptor-dependent colon development, homeostasis and inflammatory stress response.
    Authors: Huynh D, Akcora D, Malaterre J, Chan C, Dai X, Bertoncello I, Stanley E, Ramsay R
    PLoS ONE, 2013;8(2):e56951.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  24. Colony-forming cells in the adult mouse pancreas are expandable in Matrigel and form endocrine/acinar colonies in laminin hydrogel.
    Authors: Jin L, Feng T, Shih H, Zerda R, Luo A, Hsu J, Mahdavi A, Sander M, Tirrell D, Riggs A, Ku H
    Proc Natl Acad Sci U S A, 2013;110(10):3907-12.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  25. Lrig1 controls intestinal stem-cell homeostasis by negative regulation of ErbB signalling.
    Authors: Wong, Vivian W, Stange, Daniel E, Page, Mahalia, Buczacki, Simon, Wabik, Agnieszk, Itami, Satoshi, van de Wetering, Marc, Poulsom, Richard, Wright, Nicholas, Trotter, Matthew, Watt, Fiona M, Winton, Doug J, Clevers, Hans, Jensen, Kim B
    Nat Cell Biol, 2012;14(4):401-8.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: cell culture
  26. Human RSPO1/R-spondin1 is expressed during early ovary development and augments beta-catenin signaling.
    Authors: Tomaselli S, Megiorni F, Lin L, Mazzilli MC, Gerrelli D, Majore S, Grammatico P, Achermann JC
    PLoS ONE, 2011;6(1):e16366.
    Species: Human
    Sample Type: Whole Cells
    Application: Bioassay
Expand to show all 26 Citations
Recombinant Proteins
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Recombinant Mouse Wnt-3a Protein, CF

BA, BA2 1324-WN/CF 113
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Recombinant Mouse Wnt-3a Protein

BA, BA2 1324-WN 113
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 Stem/Immune cell maintenance or differentiation Anonymous 12/15/2016
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ApplicationStem/Immune cell maintenance or differentiation

Other Experimental Details

Other Experimental DetailsUsed in culture media to differentiate stem cells towards a pancreatic endocrine progenitor cell fate.

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