Recombinant Mouse SCGF/CLEC11a Protein

Carrier Free

Catalog # Availability Size / Price Qty
3729-SC-025/CF

With Carrier

Catalog # Availability Size / Price Qty
3729-SC-025
Product Details
Citations (1)
FAQs
Supplemental Products
Reviews

Recombinant Mouse SCGF/CLEC11a Protein Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to promote the expansion of E16 rat liver mononuclear cells in vitro, in the presence of Recombinant Mouse SCF/c‑kit Ligand (Catalog # 455-MC), Recombinant Mouse Thrombopoietin/Tpo (Catalog # 488-TO), and Recombinant Mouse Flt‑3 Ligand (Catalog # 427-FL). The ED50 for this effect is 0.1-0.4 μg/mL in the presence of cross-linking antibody, Mouse Anti-polyHistidine Monoclonal Antibody (Catalog # MAB050).
Source
Mouse myeloma cell line, NS0-derived mouse SCGF/CLEC11a protein
Ala22-Phe328, with a C-terminal 6-His tag
Accession #
N-terminal Sequence
Analysis
Ala22
Predicted Molecular Mass
35.1 kDa
SDS-PAGE
50 kDa, reducing conditions

Product Datasheets

3729-SC (with carrier)

3729-SC/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3729-SC

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3729-SC/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: SCGF/CLEC11a

Stem Cell Growth Factor (SCGF), also known as LSLCL, P47, and CLEC11A, is a secreted glycoprotein that acts on primitive hematopoietic stem/progenitor cells to support their proliferation and differentiation. Mature mouse SCGF contains an N‑terminal Glu‑rich region, a leucine zipper region, and a C‑terminal lectin‑like domain (1, 2). Human SCGF contains an RGD motif in its Glu‑rich region but rat SCGF does not (1). Mature mouse SCGF shares approximately 83% and 93% amino acid (aa) sequence identity with human and rat SCGF (1‑3). SCGF is produced by various hematopoietic cell lines and by CD34+ and CD34-CD33+ bone marrow cells as a 47 kDa sulfated and O‑glycosylated sialoprotein (2‑6). It is also expressed by infiltrating macrophages in some gastrointestinal tract tumors (7). Mouse SCGF mRNA has been detected in proliferating chondrocytes, the primary ossification center, perichondrium, and periosteum (6). SCGF should not be confused with the similarly named SCF (stem cell factor)/c‑Kit Ligand which exerts distinct activities during hematopoietic differentiation. SCGF exhibits erythroid burst (BFU‑E) promoting activity in the absence of SCF/c‑kit Ligand but suppresses the formation of erythroid bursts induced by SCF (4, 6). SCGF cooperates with IL‑3, Flt‑3 Ligand, GM‑CSF, or G‑CSF to enhance the growth of granulocyte‑macrophage colonies (CFU‑GM) (4, 6). It also promotes (with Flt‑3 Ligand and VEGF) the maintenance and expansion of CD34+CD133+ progenitor cells as well as their differentiation into the endothelial lineage (8, 9). Serum levels of SCGF increase during stem cell transplantation and correlate with the recovery of hematopoiesis (5). In humans, circulating SCGF levels are decreased in severe malarial anemia, and an SCGF promoter polymorphism (‑539C/T) is associated with resistance to malarial anemia (10, 11).

References
  1. Mio, H. et al. (1998) Biochem. Biophys. Res. Commun. 249:124.
  2. Bannwarth, S. et al. (1998) J. Biol. Chem. 273:1911.
  3. Hiraoka, A. et al. (1997) Proc. Natl. Acad. Sci. 94:7577.
  4. Hiraoka, A. et al. (1987) Cancer Res. 47:5025.
  5. Ito, C. et al. (2003) Bone Marrow Transplant. 32:391.
  6. Hiraoka, A. et al. (2001) Hematol. J. 2:307.
  7. Da Riva, L. et al. (2011) J. Transl. Med. 9:158.
  8. Gehling, U.M. et al. (2000) Blood 95:3106.
  9. Loges, S. et al. (2004) Stem Cells Dev. 13:229.
  10. Keller, C.C. et al. (2009) Infect. Immun. 77:3864.
  11. Ouma, C. et al. (2010) Infect. Immun. 78:453.
Long Name
Stem Cell Growth Factor/C-type Lectin Domain Family 11, Member A
Entrez Gene IDs
6320 (Human); 20256 (Mouse); 29313 (Rat)
Alternate Names
CLEC11a; CLECSF3; LSLCL; SCGF

Citation for Recombinant Mouse SCGF/CLEC11a Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Chronic activation of endothelial MAPK disrupts hematopoiesis via NFKB dependent inflammatory stress reversible by SCGF
    Authors: P Ramalingam, MG Poulos, E Lazzari, MC Gutkin, D Lopez, CC Kloss, MJ Crowley, L Katsnelson, AG Freire, MB Greenblatt, CY Park, JM Butler
    Nat Commun, 2020;11(1):666.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo

FAQs

  1. This protein datasheet indicates I need to use a cross-linking antibody, Catalog # MAB050, for biological activity. What is this antibody and is it really necessary?

    • The antibody is directed against a 6x histidine repeat and is recommended for use as a cross-linker of proteins with 6x his-tag. Crosslinking is often used for proteins that require receptor trimerization and can result greater biological activity. R&D Systems Quality Control tests the performance of these proteins in the presence of the cross-linking antibody. Therefore, it is necessary to use this antibody when trying to achieve the same level of specific activity described in the datasheet.

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