Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1004-MR-050
Product Details
Citations (7)
FAQs
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Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to bind Recombinant Mouse IL‑33 (Catalog # 3626-ML) in a functional ELISA with an estimated KD < 50 nM. Schmitz, J. et al. (2005) Immunity 23:479.
Source
Spodoptera frugiperda, Sf 21 (baculovirus)-derived mouse ST2/IL-33 R protein
Mouse ST2
(Ser27-Ser342)
Accession # P14719
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Ser27
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
63 kDa (monomer)
SDS-PAGE
73 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1004-MR

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Background: ST2/IL-33R

ST2, also known as IL-1 R4 and T1, is an Interleukin-1 receptor family glycoprotein that contributes to Th2 immune responses (1, 2). Mouse ST2 consists of a 306 amino acid (aa) extracellular domain (ECD) with three Ig-like domains, a 23 aa transmembrane segment, and a 212 aa cytoplasmic domain with an intracellular TIR domain (3). Alternate splicing of the 120 kDa mouse ST2 generates a soluble 60 kDa isoform that lacks the transmembrane and cytoplasmic regions (3). Within the ECD, mouse ST2 shares 68% and 81% aa sequence identity with human and rat ST2, respectively. ST2 is expressed on the surface of mast cells, activated Th2 cells, macrophages, and cardiac myocytes (4-7). It binds IL-33, a cytokine that is upregulated by inflammation or mechanical strain in smooth muscle cells, airway epithelia, keratinocytes, and cardiac fibroblasts (4, 8). IL-33 binding induces the association of ST2 with IL-1R AcP, a shared signaling subunit that also associates with IL-1 RI and IL-1 R rp2 (1, 9, 10). In macrophages, ST2 interferes with signaling from IL-1 RI and TLR4 by sequestering the adaptor proteins MyD88 and Mal (6). In addition to its role in promoting mast cell and Th2 dependent inflammation, ST2 activation enhances antigen induced hypernociception and protects from atherosclerosis and cardiac hypertrophy (4, 11-13). The soluble ST2 isoform is released by activated Th2 cells and strained cardiac myocytes and is elevated in the serum in allergic asthma (5, 7, 14). Soluble ST2 functions as a decoy receptor that blocks IL-33’s ability to signal through transmembrane ST2 (9, 12-14).

References
  1. Barksby, H.E. et al. (2007) Clin. Exp. Immunol. 149:217.
  2. Gadina, M. and C.A. Jefferies (2007) Science STKE 2007:pe31.
  3. Yanagisawa, K. et al. (1993) FEBS Lett. 318:83.
  4. Schmitz, J. et al. (2005) Immunity 23:479.
  5. Lecart, S. et al. (2002) Eur. J. Immunol. 32:2979.
  6. Brint, E.K. et al. (2004) Nat. Immunol. 5:373.
  7. Weinberg, E.O. et al. (2002) Circulation 106:2961.
  8. Sanada S. et al. (2007) J. Clin. Invest. 117:1538
  9. Palmer, G. et al. (2008) Cytokine 42:358.
  10. Chackerian, A.A. et al. (2007) J. Immunol. 179:2551.
  11. Allakhverdi, Z. et al. (2007) J. Immunol. 179:2051.
  12. Verri Jr., W.A. et al. (2008) Proc. Natl. Acad. Sci. 105:2723.
  13. Miller, A.M. et al. (2008) J. Exp. Med. 205:339.
  14. Hayakawa, H. et al. (2007) J. Biol. Chem. 282:26369.
Long Name
Interleukin 33 Receptor
Entrez Gene IDs
9173 (Human); 17082 (Mouse)
Alternate Names
DER4; DER4ST2growth stimulation-expressed; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33 R; IL33R; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; Ly84; MGC32623; Protein ST2; ST2; ST2L; ST2V; T1; T1homolog of mouse growth stimulation-expressed

Citations for Recombinant Mouse ST2/IL-33R Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

7 Citations: Showing 1 - 7
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  1. IL-33 ameliorates Alzheimer's disease-like pathology and cognitive decline
    Authors: AK Fu, KW Hung, MY Yuen, X Zhou, DS Mak, IC Chan, TH Cheung, B Zhang, WY Fu, FY Liew, NY Ip
    Proc Natl Acad Sci USA, 2016;0(0):.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  2. Aspirin-Exacerbated Respiratory Disease Involves a Cysteinyl Leukotriene-Driven IL-33-Mediated Mast Cell Activation Pathway.
    Authors: Liu T, Kanaoka Y, Barrett N, Feng C, Garofalo D, Lai J, Buchheit K, Bhattacharya N, Laidlaw T, Katz H, Boyce J
    J Immunol, 2016;195(8):3537-45.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  3. A robust high throughput platform to generate functional recombinant monoclonal antibodies using rabbit B cells from peripheral blood.
    Authors: Seeber S, Ros F, Thorey I, Tiefenthaler G, Kaluza K, Lifke V, Fischer J, Klostermann S, Endl J, Kopetzki E, Pashine A, Siewe B, Kaluza B, Platzer J, Offner S
    PLoS ONE, 2014;9(2):e86184.
    Species: Rabbit
    Sample Types: Cell Culture Supernates
    Applications: Bioassay
  4. Transcription factor IRF4 drives dendritic cells to promote Th2 differentiation.
    Authors: Williams, Jesse W, Tjota, Melissa, Clay, Bryan S, Vander Lugt, Bryan, Bandukwala, Hozefa S, Hrusch, Cara L, Decker, Donna C, Blaine, Kelly M, Fixsen, Bethany, Singh, Harinder, Sciammas, Roger, Sperling, Anne I
    Nat Commun, 2013;4(0):2990.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  5. The receptor tyrosine kinase c-Kit controls IL-33 receptor signaling in mast cells.
    Authors: Drube S, Heink S, Walter S, Lohn T, Grusser M, Gerbaulet A, Berod L, Schons J, Dudeck A, Freitag J, Grotha S, Reich D, Rudeschko O, Norgauer J, Hartmann K, Roers A, Kamradt T
    Blood, 2010;115(19):3899-906.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  6. IL-1 receptor accessory protein and ST2 comprise the IL-33 receptor complex.
    Authors: Chackerian AA, Oldham ER, Murphy EE, Schmitz J, Pflanz S, Kastelein RA
    J. Immunol., 2007;179(4):2551-5.
    Species: Mouse
    Sample Types: Recombinant Protein
    Applications: ELISA (Capture)
  7. IL-1 receptor accessory protein is essential for IL-33-induced activation of T lymphocytes and mast cells.
    Authors: Ali S, Huber M, Kollewe C, Bischoff SC, Falk W, Martin MU
    Proc. Natl. Acad. Sci. U.S.A., 2007;104(47):18660-5.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay

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