Recombinant Mouse TIGIT Fc Chimera Protein, CF
Recombinant Mouse TIGIT Fc Chimera Protein, CF Summary
Accession # NP_001139797
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
|Formulation||Lyophilized from a 0.2 μm filtered solution in PBS.|
|Reconstitution||Reconstitute at 100 μg/mL in PBS.|
|Shipping||The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.|
|Stability & Storage:||Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
TIGIT (T cell Immunoreceptor with Ig and ITIM domains), also called Vstm3 (V‑set and transmembrane domain‑containing 3), Vsig9 (V‑set and Ig domain-containing 9) and WUCAM (Washington University cell adhesion molecule) is a 30‑34 kDa type I transmembrane protein that is a member of the CD28 family within the Ig superfamily of proteins (1‑4). Mouse TIGIT cDNA encodes 241 amino acids (aa) including a 26 aa signal sequence, a 115 aa extracellular region with a V‑type Ig-like domain and one potential N‑glycosylation site, a 21 aa transmembrane sequence, and a 79 aa cytoplasmic domain with an ITIM motif (5). A 249 aa variant contains inserted aa after aa 21, 131 and 164 (6). Within the ECD, mouse TIGIT shares only 59‑70% aa sequence identity with human, porcine, canine, equine and bovine TIGIT. TIGIT is expressed on NK cells and subsets of activated, memory and regulatory T cells, and particularly on follicular helper T cells within secondary lymphoid organs (1, 2, 7‑9). It binds to CD155/PVR/Necl‑5 and Nectin-2/CD112/PVRL2 that appear on dendritic cells (DC) and endothelium (1‑3, 8). Binding of TIGIT by DC induces IL‑10 release and inhibits IL‑12 production (2). Ligation of TIGIT on T cells down‑regulates TCR-mediated activation and subsequent proliferation, while NK cell TIGIT ligation blocks NK cell cytotoxicity (7‑9). Through CD155 and Nectin-2, which also interact with DNAM-1/CD226 and CD96/Tactile, TIGIT is part of an interacting network of Ig superfamily members that may augment or oppose each other (3, 4, 7, 8). In particular, TIGIT binding to CD155 can antagonize the effects of DNAM‑1 (7, 8). Soluble TIGIT is able to compete with DNAM‑1 for CD155 binding and attenuates T cell responses, while mice lacking TIGIT show increased T cell responses and susceptibility to autoimmune challenges (2, 3, 9, 10).
- Boles, K.S. et al. (2009) Eur. J. Immunol. 39:695.
- Yu, X. et al. (2009) Nat. Immunol. 10:48.
- Levin, S.D. et al. (2011) Eur. J. Immunol. 41:902.
- Xu, Z. et al. (2010) Cell. Mol. Immunol. 7:11.
- NCBI Reference Sequence Accession # NP_001139797.
- Swissprot Accession # P86176.
- Seth, S. et al. (2009) Eur. J. Immunol. 39:3160.
- Stanietsky, N. et al. (2009) Proc. Natl. Acad. Sci. USA 106:17858.
- Joller, N. et al. (2011) J. Immunol. 186:1338.
- Lozano, E. et al. (2012) J. Immunol. 188: Mar. 16 [Epub ahead of print].
No product specific FAQs exist for this product, however you mayView all Proteins and Enzyme FAQs
Reviews for Recombinant Mouse TIGIT Fc Chimera Protein, CF
There are currently no reviews for this product. Be the first to review Recombinant Mouse TIGIT Fc Chimera Protein, CF and earn rewards!
Have you used Recombinant Mouse TIGIT Fc Chimera Protein, CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image