Recombinant Mouse VSIG3 Fc Chimera Protein, CF

  
  • Purity
    >95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its ability to inhibit anti-CD3 antibody induced IL-17 secretion by human peripheral blood mononuclear cells (PBMC). The ED50 for this effect is 1-5 μg/mL
  • Source
    Mouse myeloma cell line, NS0-derived
    Mouse VSIG3
    (Leu23-Gly245)
    Accession # P0C673
    IEGRMDP Mouse IgG2a
    (Glu98-Lys330)
    N-terminusC-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Leu23
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    51 kDa
  • SDS-PAGE
    60-68 kDa, reducing conditions
9367-VS
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 200 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Mouse VSIG3 Fc Chimera (Catalog # 9367-VS)inhibits anti-CD3 antibody induced IL-17 secretion in human PBMC.The ED50 for this effect is 1-5 μg/mL.
Background: VSIG3
VSIG3 (V-­set and immunoglobulin domain containing 3) also known as IgSF11 (Immunoglobulin superfamily member 11), is an approximately 50 kDa type I transmembrane adhesion protein of the Ig superfamily. Mature mouse VSIG3 consists of a 218 amino acid (aa) extracellular domain (ECD) containing one V-type Ig-like domain and one Ig-like C2-type domain, a 21 aa transmembrane domain, and a 167 aa cytoplasmic domain. Within the ECD, mouse VSIG3 shares 95% and 98% aa identity with human and rat VSIG3, respectively. VSIG3 is expressed on epithelial and endothelial cells, neurons and glial cells, and platelets (1). It localizes to epithelial tight junctions and mediates homophilic in trans cell adhesion (1-3). VSIG3 also localizes to neuronal postsynaptic densities where it recruits the GluA1 and GluA2 subunits of AMPA receptors and supports excitatory synaptic transmission (4). In zebrafish, VSIG3 is expressed in melanophores and their precursors and plays a role in the development and patterning of pigment cells (5). VSIG-3 expression is elevated in colorectal cancers and hepatocellular carcinomas as well as
intestinal-type gastric cancers. Suppression of VSIG-3 by siRNA retarded the growth of gastric cancer cells, suggesting that VSIG-3 is a good candidate for gastric cancer immunotherapy (6).
  • References:
    1. Raschperger, E. et al. (2004) J. Biol. Chem. 279:796.
    2. Katoh, M. and M. Katoh (2003) Int. J. Oncol. 23:525.
    3. Harada, H. et al. (2005) J. Cell. Physiol. 204:919.
    4. Jang, S. et al. (2016) Nat. Neurosci. 19:84.
    5. Eom, D.S. et al. (2012) PLoS Genet. 8:e1002899.
    6. Watanabe T, et al. (2005) Cancer Sci. 96:498.
  • Long Name:
    V-Set and Immunoglobulin Domain Containing 3
  • Entrez Gene IDs:
    152404 (Human); 207683 (Mouse); 303926 (Rat); 102146257 (Cynomolgus Monkey)
  • Alternate Names:
    Brain and testis-specific immunoglobulin superfamily protein; BTIGSF; BT-IgSF; cancer/testis antigen 119; CT119; CXADR like 1; CXADRL1; IGSF11; Igsf13; immunoglobulin superfamily member 11; immunoglobulin superfamily, member 11; MGC35227; V-set and immunoglobulin domain containing 3; V-set and immunoglobulin domain-containing protein 3; VSIG3; VSIG3brain and testis-specific immunoglobin superfamily protein
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