Recombinant Mouse Wnt-5b Protein

Carrier Free

Catalog # Availability Size / Price Qty
3006-WN-025/CF

With Carrier

Catalog # Availability Size / Price Qty
3006-WN-025

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Recombinant Mouse Wnt-5b Protein Summary

Product Specifications

Purity
>75%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its ability to inhibit Wnt-3a-induced alkaline phosphatase production by MC3T3‑E1 mouse preosteoblast cells. The ED50 for this effect is 0.1-0.5 µg/mL, in the presence of 5 ng/mL of Recombinant Mouse Wnt‑3a (Catalog # 1324-WN).
Optimal dilutions should be determined by each laboratory for each application.
Source
Chinese Hamster Ovary cell line, CHO-derived mouse Wnt-5b protein
Gln31-Lys372
Accession #
N-terminal Sequence
Analysis
No results obtained: Gln31 predicted
Predicted Molecular Mass
38.6 kDa
SDS-PAGE
45 kDa, reducing conditions

Product Datasheets

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3006-WN (with carrier)

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3006-WN/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

3006-WN

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

3006-WN/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS, EDTA and CHAPS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Wnt-5b

Wnt proteins are cysteine-rich secreted glycoproteins that play critical roles in both carcinogenesis and embryonic development. Wnts bind to receptors of the Frizzled family in conjunction with a coreceptor of the low-density lipoprotein receptor-related protein family (LRP-5 or -6), or the Ryk atypical receptor tyrosine kinase (1-3). Downstream effects of Wnt signaling occur through multiple pathways with differing intracellular components: the canonical Wnt/ beta -catenin pathway, the Wnt/Ca2+ pathway, and the planar cell polarity (PCP) pathway (1-4). Wnt-5b is a 49 kDa glycoprotein that is implicated in the Wnt/Ca2+ and PCP pathways (4‑8). These pathways can inhibit canonical Wnt/ beta -catenin signaling (4, 7). Human and mouse Wnt-5b are synthesized as 359 amino acid (aa) precursors that contain a 17 aa signal sequence and a 342 aa mature region. Mature mouse Wnt-5b shares 94%, 98%, 90% and 88% aa identity with mature human, rat, chick and Xenopus Wnt-5b, respectively. Although Wnt-5a and Wnt-5b share 83% aa identity, their effects may be different. For example, Wnt-5b, but not Wnt-5a, promotes cell cycle progression and is weakly transforming (8, 9). Wnt-5a and -5b are thought to coordinate developmental events, such as chondrocyte differentiation and formation of endochondral bone (5, 6, 10). In contrast to more focused expression of Wnt-5a, Wnt-5b is constitutively expressed at low levels throughout mouse embryonic development (10, 11). In adult mice, Wnt-5b is widely expressed, including heart, liver, brain, lung, testes, kidney, and pancreas (11-13). Wnt-5b is up‑regulated during early adipogenesis, and its overexpression in 3T3-L1 cells partially inhibits canonical Wnt suppression of adipogenesis (7, 14). It enhances PPAR gamma expression and promotes differentiation of preadipocytes (14). Human Wnt-5b polymorphisms have been associated with Type II diabetes (12).

References
  1. Mikels, A.J. & R. Nusse, 2006, Oncogene 25:7461.
  2. Miller, J.R. (2002) Genome Biol. 3:3001.
  3. Li, L et al., 2004, J. Neurosci 29:5873.
  4. Veeman, M.T. et al. (2003) Developmental Cell 5:367.
  5. Fazzi, R. et al. (2011) PLoS ONE 6:e25600.
  6. Bradley, E.W. and M.H. Drissi (2011) J. Cell. Physiol. 226:1683.
  7. Kanazawa, A. et al. (2005) Biochem. Biophys. Res. Com. 330:505.
  8. Wong, G.T. et al. (1994) Mol. Cell. Biol. 14:6278.
  9. Yang, Y. et al. (2003) Development 130:1003.
  10. Kemp, C. et al. (2005) Dev. Dyn. 233:1064.
  11. Gavin, B. J. et al. (1990) Genes Dev. 4:2319.
  12. Kanazawa, A. et al. (2004) Am. J. Hum. Genet. 75:832.
  13. Heller, R.S. et al. (2002) Dev. Dyn. 225:260.
  14. van Tienen, F.H.J. et al. (2009) Biochem. Biophys. Res. Commun. 387:207.
Long Name
Wingless-type MMTV Integration Site Family, Member 5b
Entrez Gene IDs
81029 (Human); 22419 (Mouse)
Alternate Names
MGC2648; protein Wnt-5b; wingless-type MMTV integration site family, member 5B; WNT-5B protein; Wnt5b; Wnt-5b

Citations for Recombinant Mouse Wnt-5b Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Oncogenic KRAS drives lipo-fibrogenesis to promote angiogenesis and colon cancer progression
    Authors: Hsu, WH;LaBella, KA;Lin, Y;Xu, P;Lee, R;Hsieh, CE;Yang, L;Zhou, A;Blecher, JM;Wu, CJ;Lin, K;Shang, X;Jiang, S;Spring, DJ;Xia, Y;Chen, P;Shen, JP;Kopetz, S;DePinho, RA;
    Cancer discovery
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Mesenchymal WNT-5A/5B Signaling Represses Lung Alveolar Epithelial Progenitors
    Authors: X Wu, EM van Dijk, JP Ng-Blichfe, IST Bos, C Ciminieri, M Königshoff, LEM Kistemaker, R Gosens
    Cells, 2019-09-25;8(10):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Diverse regulation of mammary epithelial growth and branching morphogenesis through noncanonical Wnt signaling
    Authors: K Kessenbroc, P Smith, SC Steenbeek, N Pervolarak, R Kumar, Y Minami, A Goga, L Hinck, Z Werb
    Proc. Natl. Acad. Sci. U.S.A, 2017-03-07;0(0):.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Interactions between lens epithelial and fiber cells reveal an intrinsic self-assembly mechanism.
    Authors: Dawes L, Sugiyama Y, Lovicu F, Harris C, Shelley E, McAvoy J
    Dev Biol, 2013-11-08;385(2):291-303.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Bioassay
  5. Reconstitution of a frizzled8.Wnt3a.LRP6 signaling complex reveals multiple Wnt and Dkk1 binding sites on LRP6.
    Authors: Bourhis E, Tam C, Franke Y, Bazan JF, Ernst J, Hwang J, Costa M, Cochran AG, Hannoush RN
    J. Biol. Chem., 2010-01-21;285(12):9172-9.
    Species: Human
    Sample Types: Recombinant Protein
    Applications: Binding Assay

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