Recombinant Mouse ZNRF3 Fc Chimera Protein, CF

  
  • Purity
    >95%, by SDS-PAGE with silver staining.
  • Endotoxin Level
    <0.10 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured by its binding ability in a functional ELISA. When Recombinant Mouse R‑Spondin 3 (Catalog # 4120-RS) is coated at 0.25 µg/ml, Recombinant Mouse ZNRF3 Fc Chimera binds with an apparent Kd <0.2 nM.
  • Source
    Mouse myeloma cell line, NS0-derived
    Mouse ZNRF3
    (Lys53-Met216)
    Accession # Q5SSZ7
    IEGRMDP Mouse IgG2a
    (Glu98-Lys330)
    N-terminus C-terminus
  • Accession #
  • N-terminal Sequence
    Analysis
    Lys53
  • Structure / Form
    Disulfide-linked homodimer
  • Predicted Molecular Mass
    45 kDa
  • SDS-PAGE
    51-60 kDa, reducing conditions
8328-RF
 
Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
ZNRF3 Binds R-Spondin 3.
When Recombinant Mouse R‑Spondin 3 (Catalog # 4120-RS) is coated onto a microplate well at 0.25 µg/mL, Recombinant Mouse ZNRF3 Fc Chimera (Catalog # 8328-RF) binds with an apparent Kd 0.2 nM.
1 μg/lane ofRecombinant Mouse ZNRF3(Catalog # 8328-RF)was resolved with SDS-PAGE under reducing (R) and non-reducing (NR) conditionsand visualized by silver staining, showing bands at 54.7 and138 kDa, respectively.
Background: ZNRF3
ZNRF3 (zinc/RING finger protein 3) is a member of the ZNRF3 family of type 1 transmembrane ubiquitin ligases (1). Mature mouse ZNRF3 consists of a 163 amino acid (aa) extracellular domain (ECD) with a protease associated (PA) domain fold, a short transmembrane segment, and a 675 aa cytoplasmic tail that contains a RING-type zinc finger with E3 ubiquitin ligase activity. Within the ECD, mouse and human ZNRF3 share 98% sequence identity. ZNRF3 is co-expressed on the cell surface with the homologous protein RNF43. Both proteins serve to inhibit the Wnt signaling pathway through the ubiquitination of LRP6 and a majority of the Frizzled family of Wnt receptors (1, 4, 6). ZNRF3 ubiquitin ligase activity is regulated jointly by R-Spondin and its cell surface receptors, Lgr4, 5, and 6 (2). The R-Spondin-Lgr complex binds to and facilitates the removal of the ZNRF3 from the plasma membrane, resulting in an enhancement of Wnt signaling (3, 4). Conversely, ZNRF3 can antagonize R-Spondin enhanced Wnt signaling (3, 5). ZNRF3 is highly expressed in crypt stem cells of the intestine where it modulates Wnt-induced cell proliferation to control the turnover of intestinal epithelial cells (6). ZNRF3 expression is down-regulated in gastric carcinomas, and ZNRF3 mutations are linked to carcinomas of the gastric tract, pancreas, liver, and ovary (7-10).
  • References:
    1. de Lau, W. et al. (2014) Genes Dev. 28:305.
    2. Xie, Y. et al. (2013) EMBO Rep. 14:1120.
    3. Peng, W.C. et al. (2013) PLoS One 8:e83110.
    4. Hao, H.X. et al. (2012) Nature 485:195.
    5. Moad, H.E. and A.A. Pioszak (2013) Biochemistry 52:7295.
    6. Koo, B.K. et al. (2012) Nature 488:665.
    7. Ong, C.K. et al. (2012) Nat. Genet. 44:690.
    8. Zhou, Y. et al. (2013) J. Mol. Histol. 44:555.
    9. Ryland, G.L. et al. (2013) J. Pathol. 229:469.
    10. Jiang, X. et al. (2013) Proc. Natl. Acad. Sci. USA 110:12649.
  • Long Name:
    Zinc and Ring Finger 3
  • Entrez Gene IDs:
    84133 (Human); 407821 (Mouse)
  • Alternate Names:
    BK747E2.3; KIAA1133; novel C3HC4 type Zinc finger (ring finger); RNF203; zinc and ring finger 3; zinc/RING finger protein 3; ZNRF3
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