IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The porcine IL-1 beta cDNA encodes a 267 aa precursor. A 114 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature porcine IL-1 beta shares 63%-70% aa sequence identity with canine, cotton rat, equine, feline, human, mouse, rat, and rhesus IL-1 beta.
Recombinant Porcine IL-1 beta/IL-1F2 Protein
R&D Systems | Catalog # 681-PI
Key Product Details
- R&D Systems E. coli-derived Recombinant Porcine IL-1 beta/IL-1F2 Protein (681-PI)
- Quality control testing to verify active proteins with lot specific assays by in-house scientists
- All R&D Systems proteins are covered with a 100% guarantee
Source
Accession Number
Applications
Product Specifications
Source
Ala115-Pro267
Purity
Endotoxin Level
N-terminal Sequence Analysis
Predicted Molecular Mass
Activity
The ED50 for this effect is 0.75-4.5 ng/mL.
Reviewed Applications
Read 2 reviews rated 5 using 681-PI in the following applications:
Formulation, Preparation, and Storage
Carrier Free
What does CF mean?CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
Carrier: 681-PI
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage |
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Carrier Free: 681-PI/CF
| Formulation | Lyophilized from a 0.2 μm filtered solution in PBS. |
| Reconstitution | Reconstitute at 100 μg/mL in sterile PBS. |
| Shipping | The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage |
|
Calculators
Background: IL-1 beta/IL-1F2
References
- Allan, S.M. et al. (2005) Nat. Rev. Immunol. 5:629.
- Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
- Kornman, K.S. (2006) Am. J. Clin. Nutr. 83:475S.
- Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
- Huether, M. et al. (1993) Gene 129:285.
- Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Long Name
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional IL-1 beta/IL-1F2 Products
Product Documents for Recombinant Porcine IL-1 beta/IL-1F2 Protein
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Recombinant Porcine IL-1 beta/IL-1F2 Protein
For research use only
Related Research Areas
Citations for Recombinant Porcine IL-1 beta/IL-1F2 Protein
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Customer Reviews for Recombinant Porcine IL-1 beta/IL-1F2 Protein (2)
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Customer Images
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Application: Immunoassay StandardVerified Customer | Posted 04/25/2018
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Application: Immunoassay StandardVerified Customer | Posted 11/07/2017Recombinant Porcine IL-1b (681-PI) was used as the immunoassay standard in a sandwich ELISA using the R&D antibodies MAB6811 as capture antibody and AF681 as the detection antibody.
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FAQs for Recombinant Porcine IL-1 beta/IL-1F2 Protein
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Q: Does IL-1 beta induce NO in smooth muscle?
A: Yes it does, based on the literature.
Associated Pathways
IL-1 Family Signaling Pathways
IL-9 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
IL-21 Signaling Pathways and their Primary Biological Effects in Different Immune Cell Types
Inflammasome Activation Pathways
NOD-like Receptor Signaling Pathways
Th17 Differentiation Pathway
Toll-Like Receptor Signaling Pathways
