Recombinant Porcine IL-4 Protein

Carrier Free

Catalog # Availability Size / Price Qty
654-P4-025/CF

With Carrier

Catalog # Availability Size / Price Qty
654-P4-025

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Recombinant Porcine IL-4 Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<1.0 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using TF‑1 human erythroleukemic cells. Kitamura, T. et al. (1989) J. Cell Physiol. 140:323. The ED50 for this effect is 0.15‑0.75 ng/mL.
Source
E. coli-derived porcine IL-4 protein
His25-Cys133, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
12.5 kDa

Product Datasheets

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654-P4 (with carrier)

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654-P4/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

654-P4

Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

654-P4/CF

Formulation Lyophilized from a 0.2 μm filtered solution in PBS.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: IL-4

Interleukin-4 (IL-4), also known as B cell-stimulatory factor-1, is a monomeric, approximately 13-18 kDa Th2 cytokine that shows pleiotropic effects during immune responses (1-3). It is a glycosylated polypeptide that contains three intrachain disulfide bridges and adopts a bundled four alpha -helix structure (4). Porcine IL-4 is synthesized with a 24 amino acid (aa) signal sequence. Mature porcine IL-4 shares 78%, 59%, 41%, and 41% aa sequence identity with bovine, human, mouse, and rat IL-4, respectively. Human IL-4 is active on porcine vascular endothelial cells (5). IL-4 exerts its effects through two receptor complexes (6, 7). The type I receptor, which is expressed on hematopoietic cells, is a heterodimer of the ligand binding IL-4 R alpha and the common gamma chain (a shared subunit of the receptors for IL-2, -7, -9, 
-15, and -21). The type II receptor on nonhematopoietic cells consists of IL-4 R alpha and IL-13 R alpha 1. The type II receptor also transduces IL-13 mediated signals. IL-4 is primarily expressed by Th2-biased CD4+ T cells, mast cells, basophils, and eosinophils (1, 2). It promotes cell proliferation, survival, and immunoglobulin class switch to IgE in B cells, acquisition of the Th2 phenotype by naïve CD4+ T cells, priming and chemotaxis of mast cells, eosinophils, and basophils, and the proliferation and activation of epithelial cells (8, 11). IL-4 plays a dominant role in the development of allergic inflammation and asthma (10, 12).

References
  1. Benczik, M. and S.L. Gaffen (2004) Immunol. Invest. 33:109.
  2. Chomarat, P. and J. Banchereau (1998) Int. Rev. Immunol. 17:1.
  3. Bailey, M. et al. (1993) Biochim. Biophys. Acta 1171:328.
  4. Redfield, C. et al. (1991) Biochemistry 30:11029.
  5. Stocker, C.J. et al. (2000) J. Immunol. 164:3309.
  6. Mueller, T.D. et al. (2002) Biochim. Biophys. Acta 1592:237.
  7. Nelms, K. et al. (1999) Annu. Rev. Immunol. 17:701.
  8. Paludan, S.R. (1998) Scand. J. Immunol. 48:459.
  9. Corthay, A. (2006) Scand. J. Immunol. 64:93.
  10. Ryan, J.J. et al. (2007) Crit. Rev. Immunol. 27:15.
  11. Grone, A. (2002) Vet. Immunol. Immunopathol. 88:1.
  12. Rosenberg, H.F. et al. (2007) J. Allergy Clin. Immunol. 119:1303.
Long Name
Interleukin 4
Entrez Gene IDs
3565 (Human); 16189 (Mouse); 287287 (Rat); 397225 (Porcine); 280824 (Bovine); 403785 (Canine); 574281 (Primate); 100302454 (Rabbit)
Alternate Names
B cell growth factor 1; BCDF; B-cell stimulatory factor 1; BCGF1; BCGF-1; binetrakin; BSF1; BSF-1; IL4; IL-4; IL-4B_cell stimulatory factor 1; IL4E12; interleukin 4; interleukin-4; Lymphocyte stimulatory factor 1; MGC79402; pitrakinra

Citations for Recombinant Porcine IL-4 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

18 Citations: Showing 1 - 10
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  1. Effects of IFNgamma and IL4 rich microenvironment on porcine monocyte-derived dendritic cell activation in vitro
    Authors: Z Oreskovic, L Levá, K Chlebová, K Hlavová, R Tesa?ík, J Gebauer, M Faldyna
    Research in Veterinary Science, 2022;145(0):54-62.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  2. Evaluation of the Effect of Inactivated Transmissible Gastroenteritis Virus Vaccine with Nano Silicon on the Phenotype and Function of Porcine Dendritic Cells
    Authors: L Zheng, F Zhao, J Ru, L Liu, Z Wang, N Wang, X Shu, Z Wei, H Guo
    Viruses, 2021;13(11):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Early Immune Initiation by Porcine Cells following Toxoplasma gondii Infection versus TLR Ligation
    Authors: B Hamid, J Schlosser-, L Bechtold, F Ebner, S Rausch, S Hartmann
    Microorganisms, 2021;9(9):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Lactobacillus johnsonii activates porcine monocyte derived dendritic cells maturation to modulate Th cellular immune response
    Authors: D Zheng, Z Wang, L Sui, Y Xu, L Wang, X Qiao, W Cui, Y Jiang, H Zhou, L Tang, Y Li
    Cytokine, 2021;144(0):155581.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Epigenetic Modulation of TLR4 Expression by Sulforaphane Increases Anti-Inflammatory Capacity in Porcine Monocyte-Derived Dendritic Cells
    Authors: X Qu, C Neuhoff, MU Cinar, M Pröll, E Tholen, D Tesfaye, M Hölker, K Schellande, MJ Uddin
    Biology, 2021;10(6):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Cell Culture
  6. Development and validation of a multiplex fluorescent microsphere immunoassay assay for detection of porcine cytokines
    Authors: SA Hall, SH Ison, C Owles, J Coe, DA Sandercock, AJ Zanella
    MethodsX, 2019;6(0):1218-1227.
    Species: Porcine
    Sample Types: Tissue Homogenates
    Applications: ELISA Standard
  7. Interaction of porcine monocyte-derived dendritic cells with African swine fever viruses of diverse virulence
    Authors: G Franzoni, SP Graham, G Sanna, P Angioi, MS Fiori, A Anfossi, M Amadori, S Dei Giudic, A Oggiano
    Vet. Microbiol., 2018;216(0):190-197.
    Species: Porcine
    Sample Types: Cell Culture Supernates
    Applications: Differentiation
  8. Infection of monocytes with European porcine reproductive and respiratory syndrome virus (PRRSV-1) strain Lena is significantly enhanced by dexamethasone and IL-10
    Authors: H Singleton, SP Graham, JP Frossard, KB Bodman-Smi, F Steinbach
    Virology, 2018;0(0):.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Long-term effectiveness of local BM-MSCs for skeletal muscle regeneration: a proof of concept obtained on a pig model of severe radiation burn
    Authors: C Linard, M Brachet, B L'homme, C Strup-Perr, E Busson, M Bonneau, JJ Lataillade, E Bey, M Benderitte
    Stem Cell Res Ther, 2018;9(1):299.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Innate immune responses induced by the saponin adjuvant Matrix-M in specific pathogen free pigs
    Authors: V Ahlberg, B Hjertner, P Wallgren, S Hellman, K Lövgren Be, C Fossum
    Vet. Res., 2017;48(1):30.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Protective Role of Passively Transferred Maternal Cytokines against Bordetella pertussis Infection in Newborn Piglets
    Authors: S Elahi, DR Thompson, J Van Kessel, LA Babiuk, V Gerdts
    Infect. Immun., 2017;85(4):.
    Species: Porcine
    Sample Types: Serum
    Applications: ELISA (Standard)
  12. Sulforaphane epigenetically regulates innate immune responses of porcine monocyte-derived dendritic cells induced with lipopolysaccharide.
    Authors: Qu X, Proll M, Neuhoff C, Zhang R, Cinar M, Hossain M, Tesfaye D, Grosse-Brinkhaus C, Salilew-Wondim D, Tholen E, Looft C, Holker M, Schellander K, Uddin M
    PLoS ONE, 2015;10(3):e0121574.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  13. The 15N and 46R Residues of Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Nucleocapsid Protein Enhance Regulatory T Lymphocytes Proliferation.
    Authors: Fan B, Liu X, Bai J, Li Y, Zhang Q, Jiang P
    PLoS ONE, 2015;10(9):e0138772.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  14. PRRSV-infected monocyte-derived dendritic cells express high levels of SLA-DR and CD80/86 but do not stimulate PRRSV-naive regulatory T cells to proliferate.
    Authors: Rodriguez-Gomez I, Kaser T, Gomez-Laguna J, Lamp B, Sinn L, Rumenapf T, Carrasco L, Saalmuller A, Gerner W
    Vet Res, 2015;46(0):54.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  15. The response of porcine monocyte derived macrophages and dendritic cells to Salmonella Typhimurium and lipopolysaccharide.
    Authors: Kyrova, Kamila, Stepanova, Hana, Rychlik, Ivan, Polansky, Ondrej, Leva, Lenka, Sekelova, Zuzana, Faldyna, Martin, Volf, Jiri
    BMC Vet Res, 2014;10(0):244.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  16. Genome-wide analysis of antiviral signature genes in porcine macrophages at different activation statuses.
    Authors: Sang Y, Brichalli W, Rowland R, Blecha F
    PLoS ONE, 2014;9(2):e87613.
  17. IL-4 induces protection of vascular endothelial cells against killing by complement and melittin through lipid biosynthesis.
    Authors: Black SM, Schott ME, Batdorf BH, Benson BA, Rutherford MS, Levay-Young BK, Dalmasso AP
    Eur. J. Immunol., 2010;40(3):803-12.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Cytokine responses in gnotobiotic pigs after infection with virulent or attenuated human rotavirus.
    Authors: Azevedo MS, Yuan L, Pouly S, Gonzales AM, Jeong KI, Nguyen TV, Saif LJ
    J. Virol., 2006;80(1):372-82.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)

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