Recombinant Rat EphB1 Fc Chimera Protein, CF

Catalog # Availability Size / Price Qty
1596-B1-200
R&D Systems Recombinant Proteins and Enzymes
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Citations (8)
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Recombinant Rat EphB1 Fc Chimera Protein, CF Summary

Product Specifications

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Level
<0.10 EU per 1 μg of the protein by the LAL method.
Activity
Measured by its binding ability in a functional ELISA. Immobilized Recombinant Rat EphB1 Fc Chimera at 2 µg/mL (100 µL/well) can bind recombinant mouse Ephrin‑B1 Fc Chimera with a linear range of 0.31-20 ng/mL.
Optimal dilutions should be determined by each laboratory for each application.
Source
Mouse myeloma cell line, NS0-derived rat EphB1 protein
Rat EphB1
(Met18-Gln538)
Accession # P09759
IEGRMD Human IgG1
(Pro100-Lys330)
N-terminus C-terminus
Accession #
N-terminal Sequence
Analysis
Met18
Structure / Form
Disulfide-linked homodimer
Predicted Molecular Mass
85 kDa (monomer)
SDS-PAGE
102 kDa, reducing conditions

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1596-B1

Formulation Lyophilized from a 0.2 μm filtered solution in Tris.
Reconstitution Reconstitute at 200 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
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Reconstitution Calculator

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Background: EphB1

EphB1, also known as Elk, Cek6, Net, and Hek6 (1), is a member of the Eph receptor family which binds members of the ephrin ligand family. There are two classes of receptors, designated A and B. Both the A and B class receptors have an extracellular region consisting of a globular domain, a cysteine-rich domain, and two fibronectin type III domains. This is followed by the transmembrane region and the cytoplasmic region. The cytoplasmic region contains a juxtamembrane motif with two tyrosine residues, which are the major autophosphorylation sites, a kinase domain, and a conserved sterile alpha motif (SAM) in the carboxy tail which contains one conserved tyrosine residue. Activation of kinase activity occurs after ligand recognition and binding. EphB1 has been shown to bind ephrin-B2, ephrin-B1,
ephrin‑A3, ephrin-A1, ephrin-A4, and ephrin-B3 (2, 3). The extracellular domains of human and rat EphB1 share 99% amino acid identity. Only membrane-bound or
Fc‑clustered ligands are capable of activating the receptor in vitro. While soluble monomeric ligands bind the receptor, they do not induce receptor autophosphorylation and activation (2). In vivo, the ligands and receptors display reciprocal expression (3). It has been found that nearly all the receptors and ligands are expressed in developing and adult neural tissue (3). The ephrin/Eph families also appear to play a role in angiogenesis (3).

References
  1. Eph Nomenclature Committee [letter]. (1997) Cell 90:403.
  2. Flanagan, J.G. and P. Vanderhaeghen (1998) Annu. Rev. Neurosci. 21:309.
  3. Pasquale, E.B. (1997) Curr. Opin. Cell Biol. 9:608.
Long Name
Eph Receptor B1
Entrez Gene IDs
2047 (Human); 270190 (Mouse); 24338 (Rat)
Alternate Names
Cek6; EC 2.7.10; EC 2.7.10.1; EK6; Elk; EPH receptor B1; eph tyrosine kinase 2; EphB1; EPH-like kinase 6; ephrin type-B receptor 1; EPHT2FLJ37986; Hek6; Net; NETHek6; soluble EPHB1 variant 1; Tyrosine-protein kinase receptor EPH-2

Citations for Recombinant Rat EphB1 Fc Chimera Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Targeting the Eph/Ephrin System as Anti-Inflammatory Strategy in IBD
    Authors: A Grandi, I Zini, S Palese, C Giorgio, M Tognolini, F Marchesani, S Bruno, L Flammini, AM Cantoni, R Castelli, A Lodola, A Fusari, E Barocelli, S Bertoni
    Front Pharmacol, 2019;10(0):691.
    Species: Rat
    Sample Types: Recombinant Protein
    Applications: ELISA Capture
  2. A neuroprotective astrocyte state is induced by neuronal signal EphB1 but fails in ALS models
    Authors: GE Tyzack, CE Hall, CR Sibley, T Cymes, S Forostyak, G Carlino, IF Meyer, G Schiavo, SC Zhang, GM Gibbons, J Newcombe, R Patani, A Lakatos
    Nat Commun, 2017;8(1):1164.
  3. Proteomic Analysis of a Rat Cerebral Ischemic Injury Model after Human Cerebral Endothelial Cell Transplantation
    Authors: Hyung-Seok Kim
    J Korean Neurosurg Soc, 2016;59(6):544-550.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  4. EphB4 forward signalling regulates lymphatic valve development.
    Authors: Zhang, Gu, Brady, John, Liang, Wei-Chin, Wu, Yan, Henkemeyer, Mark, Yan, Minhong
    Nat Commun, 2015;6(0):6625.
    Species: Human
    Sample Types: Antibody
    Applications: Enzyme Assay
  5. PI3K contributed to modulation of spinal nociceptive information related to ephrinBs/EphBs.
    Authors: Yu L, Zhou X, Yu J, Huang H, Jiang L, Zhang F, Cao J, Yan M
    PLoS ONE, 2012;7(8):e40930.
    Species: Mouse
    Sample Types: In Vivo
    Applications: In Vivo
  6. EphrinB2 induces tyrosine phosphorylation of NR2B via Src-family kinases during inflammatory hyperalgesia.
    Authors: Slack S, Battaglia A, Cibert-Goton V, Gavazzi I
    Neuroscience, 2008;156(1):175-83.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  7. Antiangiogenic and antitumor efficacy of EphA2 receptor antagonist.
    Authors: Dobrzanski P, Hunter K, Jones-Bolin S, Chang H, Robinson C, Pritchard S, Zhao H, Ruggeri B
    Cancer Res., 2004;64(3):910-9.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Reverse endocytosis of transmembrane ephrin-B ligands via a clathrin-mediated pathway.
    Authors: Parker M, Roberts R, Enriquez M, Zhao X, Takahashi T, Pat Cerretti D, Daniel T, Chen J
    Biochem. Biophys. Res. Commun., 2004;323(1):17-23.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay

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