Recombinant Rat IFN-gamma Protein

Carrier Free

Catalog # Availability Size / Price Qty
585-IF-100/CF

With Carrier

Catalog # Availability Size / Price Qty
585-IF-100
R&D Systems Recombinant Proteins and Enzymes
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Product Details
Citations (35)
FAQs
Reviews (4)

Recombinant Rat IFN-gamma Protein Summary

Product Specifications

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in an anti-viral assay using L‑929 mouse fibroblast cells infected with encephalomyocarditis (EMC) virus. Meager, A. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 129. The ED50 for this effect is 0.1‑0.6 ng/mL.
Source
E. coli-derived rat IFN-gamma protein
Glu23-Cys156, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
15.5 kDa

Product Datasheets

585-IF (with carrier)

585-IF/CF (carrier free)

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

585-IF

Formulation Lyophilized from a 0.2 μm filtered solution in Tris, NaCl and PEG with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

585-IF/CF

Formulation Supplied as a 0.2 μm filtered solution in Tris, NaCl and PEG 3350.
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:
  • 3 months from date of receipt, 2 to 8 °C as supplied.
Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IFN-gamma

Interferon-gamma (IFN-gamma ), also known as type II or immune interferon, exerts a wide range of immunoregulatory activities and is considered to be the prototype proinflammatory cytokine (1, 2). Mature rat IFN-gamma exists as a noncovalently linked homodimer of 20 - 25 kDa variably glycosylated subunits (3). It shares 86% amino acid sequence identity with mouse IFN-gamma and 37%‑45% with bovine, canine, cotton rat, equine, feline, human, porcine, and rhesus IFN-gamma. IFN-gamma dimers bind to IFN-gamma RI (alpha subunits) which then interact with IFN-gamma RII (beta subunits) to form the functional receptor complex of two alpha and two beta subunits. Inclusion of IFN-gamma RII increases the binding affinity for ligand and the efficiency of signal transduction (4, 5). IFN‑ gamma is produced by a variety of immune cells under inflammatory conditions, notably by T cells and NK cells (6). It plays a key role in host defense by promoting the development and activation of Th1 cells, chemoattraction and activation of monocytes and macrophages, upregulation of antigen presentation molecules, and immunoglobulin class switching in B cells. It also exhibits antiviral, antiproliferative, and apoptotic effects (6, 7). In addition, IFN-gamma functions as an anti-inflammatory mediator by promoting the development of regulatory T cells and inhibiting Th17 cell differentiation (8, 9). The pleiotropic effects of IFN-gamma contribute to the development of multiple aspects of atherosclerosis (7).

References
  1. Billiau, A. and P. Matthys (2009) Cytokine Growth Factor Rev. 20:97.
  2. Pestka, S. et al. (2004) Immunol. Rev. 202:8.
  3. Dijkema, R. et al. (1985) EMBO J. 4:761.
  4. Marsters, S.A. et al. (1995) Proc. Natl. Acad. Sci. 92:5401.
  5. Krause, C.D. et al. (2000) J. Biol. Chem. 275:22995.
  6. Schroder, K. et al. (2004) J. Leukoc. Biol. 75:163.
  7. McLaren, J.E. and D.P. Ramji (2009) Cytokine Growth Factor Rev. 20:125.
  8. Muhl, H. and J. Pfeilschifter (2003) Int. Immunopharmacol. 3:1247.
  9. Kelchtermans, H. et al. (2008) Trends Immunol. 29:479.
Long Name
Interferon gamma
Entrez Gene IDs
3458 (Human); 15978 (Mouse); 25712 (Rat); 396991 (Porcine); 281237 (Bovine); 403801 (Canine); 493965 (Feline)
Alternate Names
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma

Citations for Recombinant Rat IFN-gamma Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

35 Citations: Showing 1 - 10
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  1. Pro-inflammatory beta cell small extracellular vesicles induce beta cell failure through activation of the CXCL10/CXCR3 axis in diabetes
    Authors: N Javeed, TK Her, MR Brown, P Vanderboom, K Rakshit, AM Egan, A Vella, I Lanza, AV Matveyenko
    Cell Reports, 2021;36(8):109613.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Stimulation
  2. Astrocytes Stimulate Microglial Proliferation and M2 Polarization In Vitro through Crosstalk between Astrocytes and Microglia
    Authors: S Kim, Y Son
    International Journal of Molecular Sciences, 2021;22(16):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Genome-wide screens identify Toxoplasma gondii determinants of parasite fitness in IFN&gamma-activated murine macrophages
    Authors: Y Wang, LO Sangaré, TC Paredes-Sa, MA Hassan, S Krishnamur, AM Furuta, BM Markus, S Lourido, JPJ Saeij
    Nat Commun, 2020;11(1):5258.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Cytokine-induced translocation of GRP78 to the plasma membrane triggers a pro-apoptotic feedback loop in pancreatic beta cells
    Authors: S Vig, M Buitinga, D Rondas, I Crèvecoeur, M van Zandvo, E Waelkens, DL Eizirik, C Gysemans, P Baatsen, C Mathieu, L Overbergh
    Cell Death Dis, 2019;10(4):309.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  5. Prolactin protects against cytokine-induced beta cell death by NFkB and JNK inhibition
    Authors: TR Nardelli, EC Vanzela, KC Benedicto, F Brozzi, A Fujita, AK Cardozo, D Eizirik, AC Boschero, F Ortis
    J. Mol. Endocrinol., 2018;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  6. Analytical performance of a commercial multiplex Luminex-based cytokine panel in the rat
    Authors: T Erkens, N Goeminne, A Kegels, M Byloos, P Vinken
    J Pharmacol Toxicol Methods, 2018;91(0):43-49.
    Species: Rat
    Sample Types: Serum
    Applications: Multiplex Assay
  7. Dysfunctional autophagy following exposure to pro-inflammatory cytokines contributes to pancreatic ?-cell apoptosis
    Authors: M Lambelet, LF Terra, M Fukaya, K Meyerovich, L Labriola, AK Cardozo, F Allagnat
    Cell Death Dis, 2018;9(2):96.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  8. Microglia Responses to Pro-inflammatory Stimuli (LPS, IFN?+TNF?) and Reprogramming by Resolving Cytokines (IL-4, IL-10)
    Authors: S Lively, LC Schlichter
    Front Cell Neurosci, 2018;12(0):215.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  9. Pancreatic ?-cell protection from inflammatory stress by the endoplasmic reticulum proteins thrombospondin 1 and mesencephalic astrocyte-derived neutrotrophic factor (MANF)
    Authors: DA Cunha, M Cito, FA Grieco, C Cosentino, T Danilova, L Ladriere, M Lindahl, A Domanskyi, M Bugliani, P Marchetti, DL Eizirik, M Cnop
    J. Biol. Chem., 2017;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Y RNA fragment in extracellular vesicles confers cardioprotection via modulation of IL-10 expression and secretion
    Authors: L Cambier, G de Couto, A Ibrahim, AK Echavez, J Valle, W Liu, M Kreke, RR Smith, L Marbán, E Marbán
    EMBO Mol Med, 2017;9(3):337-352.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Wound healing protects against chemotherapy-induced alopecia in young rats via up-regulating interleukin-1?-mediated signaling
    Authors: O Stojadinov, TC Wikramanay, AC Villasante, NC Yin, L Liang, E Hinde, J Escandon, M Tomic-Cani, DM Ansell, R Paus, JJ Jimenez
    Heliyon, 2017;3(5):e00309.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  12. Targeting Cellular Calcium Homeostasis to Prevent Cytokine-Mediated Beta Cell Death
    Authors: AL Clark, K Kanekura, Z Lavagnino, LD Spears, D Abreu, J Mahadevan, T Yagi, CF Semenkovic, DW Piston, F Urano
    Sci Rep, 2017;7(1):5611.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  13. A neuropeptide, Substance-P, directly induces tissue-repairing M2 like macrophages by activating the PI3K/Akt/mTOR pathway even in the presence of IFN?
    Authors: JE Lim, E Chung, Y Son
    Sci Rep, 2017;7(1):9417.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Ubiquitin D regulates IRE1?/JNK-dependent apoptosis in pancreatic beta cells
    Authors: F Brozzi, S Gerlo, FA Grieco, M Juusola, A Balhuizen, S Lievens, C Gysemans, M Bugliani, C Mathieu, P Marchetti, J Tavernier, DL Eizirik
    J Biol Chem, 2016;0(0):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  15. HDAC inhibitor-mediated beta-cell protection against cytokine-induced toxicity is STAT1 Tyr701 phosphorylation independent.
    Authors: Dahllof M, Christensen D, Harving M, Wagner B, Mandrup-Poulsen T, Lundh M
    J Interferon Cytokine Res, 2015;35(1):63-70.
  16. HLA-B27-Homodimer-Specific Antibody Modulates the Expansion of Pro-Inflammatory T-Cells in HLA-B27 Transgenic Rats.
    Authors: Marroquin Belaunzaran O, Kleber S, Schauer S, Hausmann M, Nicholls F, van den Broek M, Payeli S, Ciurea A, Milling S, Stenner F, Shaw J, Kollnberger S, Bowness P, Petrausch U, Renner C
    PLoS ONE, 2015;10(6):e0130811.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  17. A comparative analysis of multiple sclerosis-relevant anti-inflammatory properties of ethyl pyruvate and dimethyl fumarate.
    Authors: Miljkovic D, Blazevski J, Petkovic F, Djedovic N, Momcilovic M, Stanisavljevic S, Jevtic B, Mostarica Stojkovic M, Spasojevic I
    J Immunol, 2015;194(6):2493-503.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  18. A combined "omics" approach identifies N-Myc interactor as a novel cytokine-induced regulator of IRE1 protein and c-Jun N-terminal kinase in pancreatic beta cells.
    Authors: Brozzi F, Gerlo S, Grieco F, Nardelli T, Lievens S, Gysemans C, Marselli L, Marchetti P, Mathieu C, Tavernier J, Eizirik D
    J Biol Chem, 2014;289(30):20677-93.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Linkage of inflammation and oxidative stress via release of glutathionylated peroxiredoxin-2, which acts as a danger signal.
    Authors: Salzano S, Checconi P, Hanschmann E, Lillig C, Bowler L, Chan P, Vaudry D, Mengozzi M, Coppo L, Sacre S, Atkuri K, Sahaf B, Herzenberg L, Herzenberg L, Mullen L, Ghezzi P
    Proc Natl Acad Sci U S A, 2014;111(33):12157-62.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Ceruloplasmin potentiates nitric oxide synthase activity and cytokine secretion in activated microglia.
    Authors: Lazzaro M, Bettegazzi B, Barbariga M, Codazzi F, Zacchetti D, Alessio M
    J Neuroinflammation, 2014;11(0):164.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  21. BACH2, a candidate risk gene for type 1 diabetes, regulates apoptosis in pancreatic beta-cells via JNK1 modulation and crosstalk with the candidate gene PTPN2.
    Authors: Marroqui L, Santin I, Dos Santos R, Marselli L, Marchetti P, Eizirik D
    Diabetes, 2014;63(7):2516-27.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis.
    Authors: Steelman A, Smith R, Welsh C, Li J
    J Biol Chem, 2014;288(33):23776-87.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Altering beta-cell number through stable alteration of miR-21 and miR-34a expression.
    Authors: Backe M, Novotny G, Christensen D, Grunnet L, Mandrup-Poulsen T
    Islets, 2014;6(1):e27754.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  24. EP2 receptor signaling pathways regulate classical activation of microglia.
    Authors: Quan Y, Jiang J, Dingledine R
    J Biol Chem, 2013;288(13):9293-302.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Regulation of NOX-1 expression in beta cells: a positive feedback loop involving the Src-kinase signaling pathway.
    Authors: Weaver J, Taylor-Fishwick D
    Mol Cell Endocrinol, 2013;369(1):35-41.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  26. USP18 is a key regulator of the interferon-driven gene network modulating pancreatic beta cell inflammation and apoptosis.
    Authors: Santin I, Moore F, Grieco F, Marchetti P, Brancolini C, Eizirik D
    Cell Death Dis, 2012;3(0):e419.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  27. STAT1 is a master regulator of pancreatic {beta}-cell apoptosis and islet inflammation.
    Authors: Moore F, Naamane N, Colli ML, Bouckenooghe T, Ortis F, Gurzov EN, Igoillo-Esteve M, Mathieu C, Bontempi G, Thykjaer T, Orntoft TF, Eizirik DL
    J. Biol. Chem., 2011;286(2):929-41.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  28. IL-1beta receptor blockade protects islets against pro-inflammatory cytokine induced necrosis and apoptosis.
    Authors: Schwarznau A, Hanson MS, Sperger JM, Schram BR, Danobeitia JS, Greenwood KK, Vijayan A, Fernandez LA
    J. Cell. Physiol., 2009;220(2):341-7.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Inhibition of rho family functions by lovastatin promotes myelin repair in ameliorating experimental autoimmune encephalomyelitis.
    Authors: Paintlia AS, Paintlia MK, Singh AK, Singh I
    Mol. Pharmacol., 2008;73(5):1381-93.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  30. Cytokine expression profiling of the myocardium reveals a role for CX3CL1 (fractalkine) in heart failure.
    Authors: Husberg C, Nygard S, Finsen AV, Damas JK, Frigessi A, Oie E, Waehre A, Gullestad L, Aukrust P, Yndestad A, Christensen G
    J. Mol. Cell. Cardiol., 2008;45(2):261-9.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  31. Differential ex vivo nitric oxide production by acutely isolated neonatal and adult microglia.
    Authors: Schell JB, Crane CA, Smith MF, Roberts MR
    J. Neuroimmunol., 2007;189(1):75-87.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  32. In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
    Authors: Roche M, Diamond M, Finn DP
    J. Neuroimmunol., 2006;181(1):57-67.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  33. The injury response of oligodendrocyte precursor cells is induced by platelets, macrophages and inflammation-associated cytokines.
    Authors: Rhodes KE, Raivich G, Fawcett JW
    Neuroscience, 2006;140(1):87-100.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  34. Induction and blockage of oligodendrogenesis by differently activated microglia in an animal model of multiple sclerosis.
    Authors: Butovsky O, Landa G, Kunis G, Ziv Y, Avidan H, Greenberg N, Schwartz A, Smirnov I, Pollack A, Jung S, Schwartz M
    J. Clin. Invest., 2006;116(4):905-15.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Intratumoral infusion of interleukin-1beta and interferon-gamma induces tumor invasion with macrophages and lymphocytes in a rat glioma model.
    Authors: Frewert S, Stockhammer F, Warschewske G, Zenclussen AC, Rupprecht S, Volk HD, Woiciechowsky C
    Neurosci. Lett., 2004;364(3):145-8.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

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Reviews for Recombinant Rat IFN-gamma Protein

Average Rating: 4.5 (Based on 4 Reviews)

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By Anonymous on 05/24/2022
Application: Immunoassay Standard

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Application: In vitro bioactivity in cell culture

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Reason for Rating: Works well and consistantly