Recombinant Rat IL-1 beta/IL-1F2 Protein

(37 citations)
(1 Review)
  
  • Purity
    >97%, by SDS-PAGE under reducing conditions and visualized by silver stain
  • Endotoxin Level
    <0.01 EU per 1 μg of the protein by the LAL method.
  • Activity
    Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 0.3‑1.5 ng/mL in the presence of concanavalin A.
  • Source
    E. coli-derived Val117-Ser268, with an N-terminal Met
  • Accession #
  • N-terminal Sequence
    Analysis
    Met
  • Predicted Molecular Mass
    17 kDa
Carrier Free
What does CF mean?
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
What formulation is right for me?
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
501-RL
 
501-RL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, EDTA, DTT and PEG 8000 with BSA as a carrier protein.
Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, EDTA, DTT and PEG 8000.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Data Images
Recombinant Rat IL-1 beta /IL-1F2 (Catalog # 501-RL) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 0.3-1.5 ng/mL in the presence of concanavalin A.
1 μg/lane of Recombinant Rat IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.
Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The rat IL-1 beta cDNA encodes a 268 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 65% - 77% with canine, equine, feline, human, porcine, and rhesus IL-1 beta.

  • References:
    1. Allan, S.M. et al., (2005) Nat. Rev. Immunol. 5:629.
    2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
    3. Kornman, K.S., (2006) Am. J. Clin. Nutr. 83:475S.
    4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
    5. Accession # M98820.
    6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
  • Long Name:
    Interleukin 1 beta
  • Entrez Gene IDs:
    3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
  • Alternate Names:
    catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

37 Citations: Showing 1 - 10
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Species
Applications
Sample Type
  1. Targeting Cellular Calcium Homeostasis to Prevent Cytokine-Mediated Beta Cell Death
    Authors: AL Clark, K Kanekura, Z Lavagnino, LD Spears, D Abreu, J Mahadevan, T Yagi, CF Semenkovic, DW Piston, F Urano
    Sci Rep, 2017;7(1):5611.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  2. Wound healing protects against chemotherapy-induced alopecia in young rats via up-regulating interleukin-1?-mediated signaling
    Authors: O Stojadinov, TC Wikramanay, AC Villasante, NC Yin, L Liang, E Hinde, J Escandon, M Tomic-Cani, DM Ansell, R Paus, JJ Jimenez
    Heliyon, 2017;3(5):e00309.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  3. Microglia-derived IL-1? promotes chemokine expression by M�ller cells and RPE in focal retinal degeneration
    Authors: R Natoli, N Fernando, M Madigan, JA Chu-Tan, K Valter, J Provis, M Rutar
    Mol Neurodegener, 2017;12(1):31.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  4. Retinoic-acid-orphan-receptor-C inhibition suppresses Th17 cells and induces thymic aberrations
    Authors: C Guntermann, A Piaia, ML Hamel, D Theil, T Rubic-Schn, A Del Rio-Es, L Dong, A Billich, K Kaupmann, J Dawson, K Hoegenauer, D Orain, S Hintermann, R Stringer, DD Patel, A Doelemeyer, M Deurinck, J Schümann
    JCI Insight, 2017;2(5):e91127.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  5. High-molecular-weight hyaluronic acid attenuated matrix metalloproteinase-1 and -3 expression via CD44 in tendinopathy
    Authors: PT Wu, LC Kuo, FC Su, SY Chen, TI Hsu, CY Li, KJ Tsai, IM Jou
    Sci Rep, 2017;7(0):40840.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  6. The extracellular matrix protein laminin-10 promotes blood-brain barrier repair after hypoxia and inflammation in vitro.
    Authors: Kangwantas K, Pinteaux E, Penny J
    J Neuroinflammation, 2016;13(1):25.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  7. Microglial activation enhances associative taste memory through purinergic modulation of glutamatergic neurotransmission.
    Authors: Delpech J, Saucisse N, Parkes S, Lacabanne C, Aubert A, Casenave F, Coutureau E, Sans N, Laye S, Ferreira G, Nadjar A
    J Neurosci, 2015;35(7):3022-33.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  8. HDAC inhibitor-mediated beta-cell protection against cytokine-induced toxicity is STAT1 Tyr701 phosphorylation independent.
    Authors: Dahllof M, Christensen D, Harving M, Wagner B, Mandrup-Poulsen T, Lundh M
    J Interferon Cytokine Res, 2015;35(1):63-70.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  9. Short term exposure of beta cells to low concentrations of interleukin-1beta improves insulin secretion through focal adhesion and actin remodeling and regulation of gene expression.
    Authors: Arous C, Ferreira P, Dermitzakis E, Halban P
    J Biol Chem, 2015;290(10):6653-69.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  10. Tumor necrosis factor alpha decreases glucagon-like peptide-2 expression by up-regulating G-protein-coupled receptor 120 in Crohn disease.
    Authors: Tsukahara T, Watanabe K, Watanabe T, Yamagami H, Sogawa M, Tanigawa T, Shiba M, Tominaga K, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T
    Am J Pathol, 2015;185(1):185-96.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  11. Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis.
    Authors: Steelman A, Smith R, Welsh C, Li J
    J Biol Chem, 2014;288(33):23776-87.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  12. Possible protective effect of membrane lipid rafts against interleukin-1beta-mediated anti-proliferative effect in INS-1 cells.
    Authors: Chentouf M, Guzman C, Hamze M, Gross R, Lajoix A, Peraldi-Roux S
    PLoS ONE, 2014;9(7):e102889.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  13. Pentosan polysulfate decreases myocardial expression of the extracellular matrix enzyme ADAMTS4 and improves cardiac function in vivo in rats subjected to pressure overload by aortic banding.
    Authors: Vistnes M, Aronsen J, Lunde I, Sjaastad I, Carlson C, Christensen G
    PLoS ONE, 2014;9(3):e89621.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  14. Nitric oxide and interleukin-1beta stimulate the proteasome-independent degradation of the retinoic acid hydroxylase CYP2C22 in primary rat hepatocytes.
    Authors: Lee C, Lee B, Arnold S, Isoherranen N, Morgan E
    J Pharmacol Exp Ther, 2014;348(1):141-52.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  15. Ceruloplasmin potentiates nitric oxide synthase activity and cytokine secretion in activated microglia.
    Authors: Lazzaro M, Bettegazzi B, Barbariga M, Codazzi F, Zacchetti D, Alessio M
    J Neuroinflammation, 2014;11(0):164.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  16. Combined ChIP-Seq and transcriptome analysis identifies AP-1/JunD as a primary regulator of oxidative stress and IL-1beta synthesis in macrophages.
    Authors: Hull R, Srivastava P, D'Souza Z, Atanur S, Mechta-Grigoriou F, Game L, Petretto E, Cook H, Aitman T, Behmoaras J
    BMC Genomics, 2013;14(0):92.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  17. Neuronal synapse formation induced by microglia and interleukin 10.
    Authors: Lim S, Park E, You B, Jung Y, Park A, Park S, Lee J
    PLoS ONE, 2013;8(11):e81218.
    Species: Rat
    Sample Type: Whole Cells
    Application: cell culture
  18. Regulation of NOX-1 expression in beta cells: a positive feedback loop involving the Src-kinase signaling pathway.
    Authors: Weaver J, Taylor-Fishwick D
    Mol Cell Endocrinol, 2013;369(1):35-41.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  19. Astrocytes acquire resistance to iron-dependent oxidative stress upon proinflammatory activation.
    Authors: Macco R, Pelizzoni I, Consonni A, Vitali I, Giacalone G, Martinelli Boneschi F, Codazzi F, Grohovaz F, Zacchetti D
    J Neuroinflammation, 2013;10(0):130.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  20. XOMA 052, a potent, high-affinity monoclonal antibody for the treatment of IL-1beta-mediated diseases.
    Authors: Owyang AM, Issafras H, Corbin J, Ahluwalia K, Larsen P, Pongo E, Handa M, Horwitz AH, Roell MK, Haak-Frendscho M, Masat L
    MAbs, 2011;3(1):49-60.
    Species: Human
    Sample Type: Buffer
    Application: Binding Assay
  21. Memory impairment induced by IL-1beta is reversed by alpha-MSH through central melanocortin-4 receptors.
    Authors: Gonzalez PV, Schioth HB, Lasaga M, Scimonelli TN
    Brain Behav. Immun., 2009;23(6):817-22.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  22. IL-1beta receptor blockade protects islets against pro-inflammatory cytokine induced necrosis and apoptosis.
    Authors: Schwarznau A, Hanson MS, Sperger JM, Schram BR, Danobeitia JS, Greenwood KK, Vijayan A, Fernandez LA
    J. Cell. Physiol., 2009;220(2):341-7.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  23. Interleukin-1 receptor null mutant mice show decreased anxiety-like behavior and enhanced fear memory.
    Authors: Koo JW, Duman RS
    Neurosci. Lett., 2009;456(1):39-43.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  24. Systemic immune challenge activates an intrinsically regulated local inflammatory circuit in the adrenal gland.
    Authors: Engstrom L, Rosen K, Angel A, Fyrberg A, Mackerlova L, Konsman JP, Engblom D, Blomqvist A
    Endocrinology, 2008;149(4):1436-50.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  25. Regulation of agouti-related protein messenger ribonucleic acid transcription and peptide secretion by acute and chronic inflammation.
    Authors: Scarlett JM, Zhu X, Enriori PJ, Bowe DD, Batra AK, Levasseur PR, Grant WF, Meguid MM, Cowley MA, Marks DL
    Endocrinology, 2008;149(10):4837-45.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  26. IL-1beta is an essential mediator of the antineurogenic and anhedonic effects of stress.
    Authors: Koo JW, Duman RS
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(2):751-6.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  27. Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
    Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA
    Infect. Immun., 2008;76(8):3577-86.
    Species: N/A
    Sample Type: N/A
    Application: ELISA Standard
  28. Cytokine expression profiling of the myocardium reveals a role for CX3CL1 (fractalkine) in heart failure.
    Authors: Husberg C, Nygard S, Finsen AV, Damas JK, Frigessi A, Oie E, Waehre A, Gullestad L, Aukrust P, Yndestad A, Christensen G
    J. Mol. Cell. Cardiol., 2008;45(2):261-9.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  29. Inhibition of rho family functions by lovastatin promotes myelin repair in ameliorating experimental autoimmune encephalomyelitis.
    Authors: Paintlia AS, Paintlia MK, Singh AK, Singh I
    Mol. Pharmacol., 2008;73(5):1381-93.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  30. Interleukin-18-mediated microglia/astrocyte interaction in the spinal cord enhances neuropathic pain processing after nerve injury.
    Authors: Miyoshi K, Obata K, Kondo T, Okamura H, Noguchi K
    J. Neurosci., 2008;28(48):12775-87.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  31. In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
    Authors: Roche M, Diamond M, Finn DP
    J. Neuroimmunol., 2006;181(1):57-67.
  32. Inflammatory signalling pathways involved in astroglial activation by unconjugated bilirubin.
    Authors: Fernandes A, Falcao AS, Silva RF, Gordo AC, Gama MJ, Brito MA, Brites D
    J. Neurochem., 2006;96(6):1667-79.
    Species: Rat
    Sample Type: Whole Cells
    Application: Bioassay
  33. Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
    Authors: Barkho BZ, Song H, Aimone JB
    Stem Cells Dev., 2006;15(3):407-21.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Bioassay
  34. A synthetic VIP peptide analogue inhibits neutrophil recruitment in rat airways in vivo.
    Authors: Sergejeva S, Hoshino H, Yoshihara S, Kashimoto K, Lotvall J, Linden A
    Regul. Pept., 2004;117(2):149-54.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  35. Intratumoral infusion of interleukin-1beta and interferon-gamma induces tumor invasion with macrophages and lymphocytes in a rat glioma model.
    Authors: Frewert S, Stockhammer F, Warschewske G, Zenclussen AC, Rupprecht S, Volk HD, Woiciechowsky C
    Neurosci. Lett., 2004;364(3):145-8.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  36. Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats.
    Authors: Nedrebo T, Reed RK, Jonsson R, Berg A, Wiig H
    J. Physiol. (Lond.), 2004;556(0):193-202.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
  37. In altering the release of glucocorticoids, ketorolac exacerbates the effects of systemic immune stimuli on expression of proinflammatory genes in the brain.
    Authors: Blais V, Zhang J, Rivest S
    Endocrinology, 2002;143(12):4820-7.
    Species: Rat
    Sample Type: In Vivo
    Application: In Vivo
Expand to show all 37 Citations
Average Rating: 5 (Based on 1 review)

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Immunoassay Standard Recombinant Rat IL-1 beta/IL-1F2 Protein 501-RL
Immunoassay Standard: Recombinant Rat IL-1 beta/IL-1F2 Protein [501-RL]

Summary

ApplicationImmunoassay Standard

Other Experimental Details

Other Experimental DetailsThis recombinant protein worked very well as a calibrator in an ELISA built to measure Rat serum IL-1b.

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