Recombinant Rat IL-1 beta/IL-1F2 Protein

R&D Systems | Catalog # 501-RL

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Key Product Details

  • R&D Systems E. coli-derived Recombinant Rat IL-1 beta/IL-1F2 Protein (501-RL)
  • Quality control testing to verify active proteins with lot specific assays by in-house scientists
  • All R&D Systems proteins are covered with a 100% guarantee

Source

E. coli

Accession Number

Q63264

Applications

Bioactivity
View all IL-1 beta/IL-1F2 Proteins and Enzymes »

Product Specifications

Source

E. coli-derived rat IL-1 beta/IL-1F2 protein
Val117-Ser268, with an N-terminal Met

Purity

>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<0.01 EU per 1 μg of the protein by the LAL method.

N-terminal Sequence Analysis

Met

Predicted Molecular Mass

17 kDa

Activity

Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272.
The ED50 for this effect is 0.300‑1.50 ng/mL.

Reviewed Applications

Read 1 review rated 5 using 501-RL in the following applications:

Scientific Data Images for Recombinant Rat IL-1 beta/IL-1F2 Protein

Recombinant Rat IL-1 beta/IL-1F2 Protein Bioactivity

Recombinant Rat IL-1 beta/IL-1F2 Protein Bioactivity

Recombinant Rat IL-1 beta /IL-1F2 (Catalog # 501-RL) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 0.300-1.50 ng/mL.
Recombinant Rat IL-1 beta/IL-1F2 Protein SDS-PAGE

Recombinant Rat IL-1 beta/IL-1F2 Protein SDS-PAGE

1 μg/lane of Recombinant Rat IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.

Formulation, Preparation, and Storage

Carrier Free
What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

Carrier: 501-RL
Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, EDTA, DTT and PEG 8000 with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Carrier Free: 501-RL/CF
Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, EDTA, DTT and PEG 8000.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The rat IL-1 beta cDNA encodes a 268 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 65% - 77% with canine, equine, feline, human, porcine, and rhesus IL-1 beta.

References

  1. Allan, S.M. et al., (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S., (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Accession # M98820.
  6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.

Long Name

Interleukin 1 beta

Alternate Names

IL-1b, IL-1F2, IL1 beta, IL1B

Entrez Gene IDs

3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)

Gene Symbol

IL1B

UniProt

Q63264

Additional IL-1 beta/IL-1F2 Products

Product Documents for Recombinant Rat IL-1 beta/IL-1F2 Protein

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS 501-RL (with carrier)
Download SDS 501-RL/CF (carrier free)

Product Specific Notices for Recombinant Rat IL-1 beta/IL-1F2 Protein

For research use only

Citations for Recombinant Rat IL-1 beta/IL-1F2 Protein

Customer Reviews for Recombinant Rat IL-1 beta/IL-1F2 Protein (1)

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  • Recombinant Rat IL-1 beta/IL-1F2 Protein
    Name: Anonymous
    Application: Immunoassay Standard
    Verified Customer | Posted 11/07/2017
    This recombinant protein worked very well as a calibrator in an ELISA built to measure Rat serum IL-1b.
    Recombinant Rat IL-1 beta/IL-1F2 Protein 501-RL

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FAQs for Recombinant Rat IL-1 beta/IL-1F2 Protein

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  • Q: Does IL-1 beta induce NO in smooth muscle?

    A: Yes it does, based on the literature.

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