Recombinant Rat IL-1 beta/IL-1F2 Protein

Formulations:
Catalog # Availability Size / Price Qty
501-RL-010
501-RL-050
Recombinant Rat IL-1 beta/IL-1F2 Protein Data
2 Images
Product Details
Citations (44)
FAQs
Reviews (1)

Recombinant Rat IL-1 beta/IL-1F2 Protein Summary

Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Level
<0.01 EU per 1 μg of the protein by the LAL method.
Activity
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 0.3‑1.5 ng/mL in the presence of concanavalin A.
Source
E. coli-derived rat IL-1 beta/IL-1F2 protein
Val117-Ser268, with an N-terminal Met
Accession #
N-terminal Sequence
Analysis
Met
Predicted Molecular Mass
17 kDa

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

501-RL

Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, EDTA, DTT and PEG 8000 with BSA as a carrier protein.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

501-RL/CF

Formulation Lyophilized from a 0.2 μm filtered solution in MES, NaCl, EDTA, DTT and PEG 8000.
Reconstitution Reconstitute at 100 μg/mL in sterile PBS.
Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

Data Images

Bioactivity View Larger

Recombinant Rat IL-1 beta /IL-1F2 (Catalog # 501-RL) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50for this effect is 0.3-1.5 ng/mL in the presence of concanavalin A.

SDS-PAGE View Larger

1 μg/lane of Recombinant Rat IL-1 beta /IL-1F2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.

Reconstitution Calculator

Reconstitution Calculator

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Background: IL-1 beta/IL-1F2

IL-1 is a name that designates two pleiotropic cytokines, IL-1 alpha (IL-1F1) and IL-1 beta (IL-1F2), which are the products of distinct genes. IL-1 alpha and IL-1 beta are structurally related polypeptides that share approximately 26% amino acid (aa) identity in rat. Both proteins are produced by a wide variety of cells in response to inflammatory agents, infections, or microbial endotoxins. While IL-1 alpha and IL-1 beta are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1 RI binds directly to IL-1 alpha or IL-1 beta and then associates with IL-1 R accessory protein (IL-1 R3/IL-1 R AcP) to form a high-affinity receptor complex that is competent for signal transduction. IL-1 RII has high affinity for IL-1 beta but functions as a decoy receptor and negative regulator of IL-1 beta activity. IL-1ra functions as a competitive antagonist by preventing IL-1 alpha and IL-1 beta from interacting with IL-1 RI (1 - 4). The rat IL-1 beta cDNA encodes a 268 aa precursor. A 116 aa propeptide is cleaved intracellularly by the cysteine protease IL-1 beta -converting enzyme (Caspase-1/ICE) to generate the active cytokine (5, 6). The 17 kDa mature rat IL-1 beta shares 90% aa sequence identity with cotton rat and mouse and 65% - 77% with canine, equine, feline, human, porcine, and rhesus IL-1 beta.

References
  1. Allan, S.M. et al., (2005) Nat. Rev. Immunol. 5:629.
  2. Boraschi, D. and A. Tagliabue (2006) Vitam. Horm. 74:229.
  3. Kornman, K.S., (2006) Am. J. Clin. Nutr. 83:475S.
  4. Isoda, K. and F. Ohsuzu (2006) J. Atheroscler. Thromb. 13:21.
  5. Accession # M98820.
  6. Martinon, F. and J. Tschopp (2007) Cell Death Differ. 14:10.
Long Name
Interleukin 1 beta
Entrez Gene IDs
3553 (Human); 16176 (Mouse); 24494 (Rat); 397122 (Porcine); 403974 (Canine); 100034237 (Equine); 100135556 (Guinea Pig)
Alternate Names
catabolin; IL1 beta; IL-1 beta; IL-1; IL1B; IL-1b; IL1-BETA; IL-1F2; IL1F2IL-1 beta; interleukin 1, beta; interleukin-1 beta; preinterleukin 1 beta; pro-interleukin-1-beta

Citations for Recombinant Rat IL-1 beta/IL-1F2 Protein

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

44 Citations: Showing 1 - 10
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  1. Phytoestrogen Agathisflavone Ameliorates Neuroinflammation-Induced by LPS and IL-1&beta and Protects Neurons in Cocultures of Glia/Neurons
    Authors: MMA de Almeida, CDS Souza, NS Dourado, AB da Silva, RS Ferreira, JM David, JP David, MFD Costa, VDA da Silva, AM Butt, SL Costa
    Biomolecules, 2020;10(4):.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  2. The pro-inflammatory effect of NR4A3 in osteoarthritis
    Authors: C Ma, L Wu, L Song, Y He, S Adel Abdo, S Yan, K Sheng, H Wu, J Ran, L Wu
    J. Cell. Mol. Med., 2019;0(0):.
    Species: Rat
    Sample Types: Whole Cell
    Applications: Bioassay
  3. Sfrp5 increases glucose-stimulated insulin secretion in the rat pancreatic beta cell line INS-1E
    Authors: M Carstensen, K Röhrig, C Niersmann, DM Ouwens, BF Belgardt, M Roden, C Herder
    PLoS ONE, 2019;14(3):e0213650.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  4. Differential regulation of GSK-3? in spinal dorsal horn and in hippocampus mediated by IL-1? contributes to pain hypersensitivity and memory deficits following peripheral nerve injury
    Authors: CL Mai, X Wei, YN Xu, J Zhang, ZJ Lin, Z Tan, YT Meng, YY Li, LJ Zhou, XG Liu
    Mol Pain, 2019;0(0):1744806919826.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  5. MicroRNA-128a represses chondrocyte autophagy and exacerbates knee osteoarthritis by disrupting Atg12
    Authors: WS Lian, JY Ko, RW Wu, YC Sun, YS Chen, SL Wu, LH Weng, H Jahr, FS Wang
    Cell Death Dis, 2018;9(9):919.
    Species: Rat
    Sample Types: Whole Cells
  6. Targeting Cellular Calcium Homeostasis to Prevent Cytokine-Mediated Beta Cell Death
    Authors: AL Clark, K Kanekura, Z Lavagnino, LD Spears, D Abreu, J Mahadevan, T Yagi, CF Semenkovic, DW Piston, F Urano
    Sci Rep, 2017;7(1):5611.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  7. Wound healing protects against chemotherapy-induced alopecia in young rats via up-regulating interleukin-1?-mediated signaling
    Authors: O Stojadinov, TC Wikramanay, AC Villasante, NC Yin, L Liang, E Hinde, J Escandon, M Tomic-Cani, DM Ansell, R Paus, JJ Jimenez
    Heliyon, 2017;3(5):e00309.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  8. Microglia-derived IL-1? promotes chemokine expression by M�ller cells and RPE in focal retinal degeneration
    Authors: R Natoli, N Fernando, M Madigan, JA Chu-Tan, K Valter, J Provis, M Rutar
    Mol Neurodegener, 2017;12(1):31.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  9. High-molecular-weight hyaluronic acid attenuated matrix metalloproteinase-1 and -3 expression via CD44 in tendinopathy
    Authors: PT Wu, LC Kuo, FC Su, SY Chen, TI Hsu, CY Li, KJ Tsai, IM Jou
    Sci Rep, 2017;7(0):40840.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  10. Retinoic-acid-orphan-receptor-C inhibition suppresses Th17 cells and induces thymic aberrations
    Authors: C Guntermann, A Piaia, ML Hamel, D Theil, T Rubic-Schn, A Del Rio-Es, L Dong, A Billich, K Kaupmann, J Dawson, K Hoegenauer, D Orain, S Hintermann, R Stringer, DD Patel, A Doelemeyer, M Deurinck, J Schümann
    JCI Insight, 2017;2(5):e91127.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  11. Pharmacological inhibition of ROR?t suppresses the Th17 pathway and alleviates arthritis in vivo
    Authors: U Guendisch, J Weiss, F Ecoeur, JC Riker, K Kaupmann, J Kallen, S Hintermann, D Orain, J Dawson, A Billich, C Guntermann
    PLoS ONE, 2017;12(11):e0188391.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  12. Expression of adiponectin receptors in human and rat intervertebral disc cells and changes in receptor expression during disc degeneration using a rat tail temporary static compression model
    Authors: Kotaro Nishida
    J Orthop Surg Res, 2016;11(1):147.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  13. The extracellular matrix protein laminin-10 promotes blood-brain barrier repair after hypoxia and inflammation in vitro.
    Authors: Kangwantas K, Pinteaux E, Penny J
    J Neuroinflammation, 2016;13(1):25.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  14. Tumor necrosis factor alpha decreases glucagon-like peptide-2 expression by up-regulating G-protein-coupled receptor 120 in Crohn disease.
    Authors: Tsukahara T, Watanabe K, Watanabe T, Yamagami H, Sogawa M, Tanigawa T, Shiba M, Tominaga K, Fujiwara Y, Maeda K, Hirakawa K, Arakawa T
    Am J Pathol, 2015;185(1):185-96.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  15. Microglial activation enhances associative taste memory through purinergic modulation of glutamatergic neurotransmission.
    Authors: Delpech J, Saucisse N, Parkes S, Lacabanne C, Aubert A, Casenave F, Coutureau E, Sans N, Laye S, Ferreira G, Nadjar A
    J Neurosci, 2015;35(7):3022-33.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  16. Short term exposure of beta cells to low concentrations of interleukin-1beta improves insulin secretion through focal adhesion and actin remodeling and regulation of gene expression.
    Authors: Arous C, Ferreira P, Dermitzakis E, Halban P
    J Biol Chem, 2015;290(10):6653-69.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  17. HDAC inhibitor-mediated beta-cell protection against cytokine-induced toxicity is STAT1 Tyr701 phosphorylation independent.
    Authors: Dahllof M, Christensen D, Harving M, Wagner B, Mandrup-Poulsen T, Lundh M
    J Interferon Cytokine Res, 2015;35(1):63-70.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  18. Pentosan polysulfate decreases myocardial expression of the extracellular matrix enzyme ADAMTS4 and improves cardiac function in vivo in rats subjected to pressure overload by aortic banding.
    Authors: Vistnes M, Aronsen J, Lunde I, Sjaastad I, Carlson C, Christensen G
    PLoS ONE, 2014;9(3):e89621.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  19. Possible protective effect of membrane lipid rafts against interleukin-1beta-mediated anti-proliferative effect in INS-1 cells.
    Authors: Chentouf M, Guzman C, Hamze M, Gross R, Lajoix A, Peraldi-Roux S
    PLoS ONE, 2014;9(7):e102889.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  20. Ceruloplasmin potentiates nitric oxide synthase activity and cytokine secretion in activated microglia.
    Authors: Lazzaro M, Bettegazzi B, Barbariga M, Codazzi F, Zacchetti D, Alessio M
    J Neuroinflammation, 2014;11(0):164.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  21. Nitric oxide and interleukin-1beta stimulate the proteasome-independent degradation of the retinoic acid hydroxylase CYP2C22 in primary rat hepatocytes.
    Authors: Lee C, Lee B, Arnold S, Isoherranen N, Morgan E
    J Pharmacol Exp Ther, 2014;348(1):141-52.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  22. Galectin-9 protein is up-regulated in astrocytes by tumor necrosis factor and promotes encephalitogenic T-cell apoptosis.
    Authors: Steelman A, Smith R, Welsh C, Li J
    J Biol Chem, 2014;288(33):23776-87.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  23. Combined ChIP-Seq and transcriptome analysis identifies AP-1/JunD as a primary regulator of oxidative stress and IL-1beta synthesis in macrophages.
    Authors: Hull R, Srivastava P, D'Souza Z, Atanur S, Mechta-Grigoriou F, Game L, Petretto E, Cook H, Aitman T, Behmoaras J
    BMC Genomics, 2013;14(0):92.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  24. Regulation of NOX-1 expression in beta cells: a positive feedback loop involving the Src-kinase signaling pathway.
    Authors: Weaver J, Taylor-Fishwick D
    Mol Cell Endocrinol, 2013;369(1):35-41.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  25. Astrocytes acquire resistance to iron-dependent oxidative stress upon proinflammatory activation.
    Authors: Macco R, Pelizzoni I, Consonni A, Vitali I, Giacalone G, Martinelli Boneschi F, Codazzi F, Grohovaz F, Zacchetti D
    J Neuroinflammation, 2013;10(0):130.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  26. Neuronal synapse formation induced by microglia and interleukin 10.
    Authors: Lim S, Park E, You B, Jung Y, Park A, Park S, Lee J
    PLoS ONE, 2013;8(11):e81218.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Cell Culture
  27. XOMA 052, a potent, high-affinity monoclonal antibody for the treatment of IL-1beta-mediated diseases.
    Authors: Owyang AM, Issafras H, Corbin J, Ahluwalia K, Larsen P, Pongo E, Handa M, Horwitz AH, Roell MK, Haak-Frendscho M, Masat L
    MAbs, 2011;3(1):49-60.
    Species: Human
    Sample Types: Buffer
    Applications: Binding Assay
  28. IL-1beta receptor blockade protects islets against pro-inflammatory cytokine induced necrosis and apoptosis.
    Authors: Schwarznau A, Hanson MS, Sperger JM, Schram BR, Danobeitia JS, Greenwood KK, Vijayan A, Fernandez LA
    J. Cell. Physiol., 2009;220(2):341-7.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  29. Interleukin-1 receptor null mutant mice show decreased anxiety-like behavior and enhanced fear memory.
    Authors: Koo JW, Duman RS
    Neurosci. Lett., 2009;456(1):39-43.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  30. Memory impairment induced by IL-1beta is reversed by alpha-MSH through central melanocortin-4 receptors.
    Authors: Gonzalez PV, Schioth HB, Lasaga M, Scimonelli TN
    Brain Behav. Immun., 2009;23(6):817-22.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  31. Rat pneumonia and soft-tissue infection models for the study of Acinetobacter baumannii biology.
    Authors: Russo TA, Beanan JM, Olson R, MacDonald U, Luke NR, Gill SR, Campagnari AA
    Infect. Immun., 2008;76(8):3577-86.
    Species: N/A
    Sample Types: N/A
    Applications: ELISA (Standard)
  32. Regulation of agouti-related protein messenger ribonucleic acid transcription and peptide secretion by acute and chronic inflammation.
    Authors: Scarlett JM, Zhu X, Enriori PJ, Bowe DD, Batra AK, Levasseur PR, Grant WF, Meguid MM, Cowley MA, Marks DL
    Endocrinology, 2008;149(10):4837-45.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  33. IL-1beta is an essential mediator of the antineurogenic and anhedonic effects of stress.
    Authors: Koo JW, Duman RS
    Proc. Natl. Acad. Sci. U.S.A., 2008;105(2):751-6.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  34. Cytokine expression profiling of the myocardium reveals a role for CX3CL1 (fractalkine) in heart failure.
    Authors: Husberg C, Nygard S, Finsen AV, Damas JK, Frigessi A, Oie E, Waehre A, Gullestad L, Aukrust P, Yndestad A, Christensen G
    J. Mol. Cell. Cardiol., 2008;45(2):261-9.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  35. Inhibition of rho family functions by lovastatin promotes myelin repair in ameliorating experimental autoimmune encephalomyelitis.
    Authors: Paintlia AS, Paintlia MK, Singh AK, Singh I
    Mol. Pharmacol., 2008;73(5):1381-93.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  36. Systemic immune challenge activates an intrinsically regulated local inflammatory circuit in the adrenal gland.
    Authors: Engstrom L, Rosen K, Angel A, Fyrberg A, Mackerlova L, Konsman JP, Engblom D, Blomqvist A
    Endocrinology, 2008;149(4):1436-50.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  37. Interleukin-18-mediated microglia/astrocyte interaction in the spinal cord enhances neuropathic pain processing after nerve injury.
    Authors: Miyoshi K, Obata K, Kondo T, Okamura H, Noguchi K
    J. Neurosci., 2008;28(48):12775-87.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  38. In vivo modulation of LPS-induced alterations in brain and peripheral cytokines and HPA axis activity by cannabinoids.
    Authors: Roche M, Diamond M, Finn DP
    J. Neuroimmunol., 2006;181(1):57-67.
  39. Inflammatory signalling pathways involved in astroglial activation by unconjugated bilirubin.
    Authors: Fernandes A, Falcao AS, Silva RF, Gordo AC, Gama MJ, Brito MA, Brites D
    J. Neurochem., 2006;96(6):1667-79.
    Species: Rat
    Sample Types: Whole Cells
    Applications: Bioassay
  40. Identification of astrocyte-expressed factors that modulate neural stem/progenitor cell differentiation.
    Authors: Barkho BZ, Song H, Aimone JB
    Stem Cells Dev., 2006;15(3):407-21.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Bioassay
  41. Differential cytokine response in interstitial fluid in skin and serum during experimental inflammation in rats.
    Authors: Nedrebo T, Reed RK, Jonsson R, Berg A, Wiig H
    J. Physiol. (Lond.), 2004;556(0):193-202.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  42. A synthetic VIP peptide analogue inhibits neutrophil recruitment in rat airways in vivo.
    Authors: Sergejeva S, Hoshino H, Yoshihara S, Kashimoto K, Lotvall J, Linden A
    Regul. Pept., 2004;117(2):149-54.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  43. Intratumoral infusion of interleukin-1beta and interferon-gamma induces tumor invasion with macrophages and lymphocytes in a rat glioma model.
    Authors: Frewert S, Stockhammer F, Warschewske G, Zenclussen AC, Rupprecht S, Volk HD, Woiciechowsky C
    Neurosci. Lett., 2004;364(3):145-8.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo
  44. In altering the release of glucocorticoids, ketorolac exacerbates the effects of systemic immune stimuli on expression of proinflammatory genes in the brain.
    Authors: Blais V, Zhang J, Rivest S
    Endocrinology, 2002;143(12):4820-7.
    Species: Rat
    Sample Types: In Vivo
    Applications: In Vivo

FAQs

  1. Does IL-1 beta induce NO in smooth muscle?

    • Yes it does, based on the literature.

View all Proteins and Enzyme FAQs

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Recombinant Rat IL-1 beta/IL-1F2 Protein
By Anonymous on 11/07/2017
Application: Immunoassay Standard

This recombinant protein worked very well as a calibrator in an ELISA built to measure Rat serum IL-1b.