Recombinant Yeast GST-SUMO E1 (SAE1/UBA2) Protein, CF

Catalog # Availability Size / Price Qty
E-310-050
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Recombinant Yeast GST-SUMO E1 (SAE1/UBA2) Protein, CF Summary

Purity
>95%, by SDS-PAGE under reducing conditions and visualized by Colloidal Coomassie® Blue stain
Activity
S. cerevisiae GST-SUMO E1 (SAE1/UBA2) is a member of the SUMO-activating (E1) enzyme family that is required for the first step of the enzymatic cascade that subsequently utilizes a SUMO-conjugating (E2) enzyme to conjugate SUMO to substrate proteins. A SUMO ligase (E3) is sometimes utilized for SUMO conjugation, but is not always required. Reaction conditions will need to be optimized for each specific application. We recommend an initial S. cerevisiae GST-SUMO E1 (SAE1/UBA2) concentration of 50-500 nM.
Source
E. coli-derived yeast SUMO Activating Enzyme E1 (SAE1/UBA2) protein
Contains an N-terminal GST (glutathione S-transferase) tag
Accession #

Product Datasheets

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

E-310

Shipping The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -70 °C as supplied.
  • 3 months, -70 °C under sterile conditions after opening.
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Background: SUMO Activating Enzyme E1 (SAE1/UBA2)

Small Ubiquitin-like Modifier (SUMO) Activating Enzyme Subunit 1 (SAE1) is the highly conserved human ortholog of yeast Ubiquitin-activating enzyme E1-like (UBA2) (1). These SUMO-activating (E1) enzymes are critical for the enzymatic attachment of SUMO molecules to a target protein by a post-translational modification process termed SUMOylation (2-4).  The ATP-dependent E1 enzyme charges the SUMO by forming a high-energy thiol ester intermediate which is transferred to the UBE2I/Ubc9 SUMO-conjugating (E2) enzyme (5,6). The second step is the trans-esterification reaction whereby SUMO is transferred to Cys93 of UbcH9. UBE2I/Ubc9 is the only known E2 that is able to mediate the conjugation of SUMO to lysine residues on a variety of cellular targets, usually in the absence of a Ubiquitin ligase (E3). Although UBE2I/Ubc9 can directly recognize and modify lysine residues contained in a SUMOylation motif, E3-like factors most likely facilitate the SUMOylation of specific substrates.

Conjugation of the ubiquitin-like modifier SUMO requires the activities of the heterodimeric E1 (Aos1/Uba2) and the UbcH9 E2 enzyme. The dimeric activating enzyme utilizes ATP to adenylate the C-terminal glycine residue of SUMO-1 (also SUMO-2 and SUMO-3), forming a high-energy thiolester bond with the cysteine residue of Uba2 and the release of AMP and PPi. The second step is the trans-esterification reaction whereby SUMO-1 is transferred to Cys93 of UbcH9.

References
  1. Johnson, E.S. et al. (1997) EMBO J. 16:5509.
  2. Desterro, J.M. et al. (1997) FEBs. Lett. 417:297.
  3. Bettermann, K. et al. (2012) Cancer Lett. 316:113.
  4. Praefcke, G.J. et al. (2012) Trends Biochem. Sci. 37:23.
  5. Okuma, T. et al. (1999) Biochem. Biophys. Res. Commun. 254:693.
  6. Tatham, M.H. et al. (2001) J. Biol. Chem. 276:35368.
Long Name
SUMO1 Activating Enzyme E1
Entrez Gene IDs
10055 (Human); 56459 (Mouse); 308384 (Rat); 856310 (Yeast)
Alternate Names
AOS1; AOS1activator of SUMO1; FLJ3091; HSPC140; SAE1; SUA1; SUA1sentrin/SUMO-activating protein AOS1; SUMO Activating Enzyme E1 (SAE1/UBA2); SUMO-1 activating enzyme E1 N subunit; SUMO1 activating enzyme subunit 1; SUMO-1 activating enzyme subunit 1; SUMO-activating enzyme subunit 1; UBA2; Ubiquitin-like 1-activating enzyme E1A; ubiquitin-like protein SUMO-1 activating enzyme; UBLE1A

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