Recombinant Yeast Ubiquitin Activating Enzyme (UBE1), CF
Discontinued Product
Recombinant Yeast Ubiquitin Activating Enzyme (UBE1), CF Summary
Product Specifications
Product Datasheets
Carrier Free
CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.
In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.
E-301
| Shipping | The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below. |
| Stability & Storage: | Store the unopened product at -70 °C. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Do not use past expiration date. |
Reconstitution Calculator
Background: Ubiquitin-activating Enzyme/UBE1
Ubiquitin-activating Enzyme (UBE1), also known as Ubiquitin-like Modifier Activating Enzyme 1 (UBA1), is a 1058 amino acid (aa) canonical member of the Ubiquitin-activating (E1) enzyme family of proteins with a predicted molecular weight of 118 kDa. It is ubiquitously expressed and highly conserved; mouse and rat UBE1 share 95% and 96% aa sequence identity with the human UBE1 protein, respectively. UBE1 is found in the cytoplasm and nucleus, and contains a conserved active-site cysteine residue and ATP-binding site common to E1 enzymes. UBE1 is responsible for the first step in Ubiquitin-protein isopeptide bond formation. Ubiquitin is activated by UBE1 and thereafter linked to the side chain of a cysteine residue in UBE1, Cys632 in humans, yielding a Ubiquitin-UBE1 conjugate via a thioester bond. The activated Ubiquitin is then transferred to a lysine residue on the target protein via the Ubiquitin-conjugating - Ubiquitin ligase enzyme cascade. UBE1 is required for cell cycle progression and has been linked to cellular responses to DNA damage such as nucleotide excision repair. Mutations in UBE1 are associated with X-linked lethal infantile spinal muscular atrophy. UBE1 is a critical component for the initiation of in vitro ubiquitin conjugation reactions.
- Ciechanover A. et al. (1982) J. Biol. Chem. 257:2537-2542
- Ghaboosi N. and Deshaies R. J. (2007) Mol. Biol. Cell. 181:1953-1963
- Haas A. L. and Rose I. A. (1982) J. Biol. Chem. 257:1032910337
- Haas A. L. et al. (1982) J. Biol. Chem. 257:2543-2548
- Handley P. M. et al. (1991) Proc. Natl. Acad. Sci. 88:258-262
- Hoefer M and Cook J. C. (1991) FEBS. Lett. 289:54-59
- Jonnalagadda S. et al. (1988) J. Biol. Chem. 263:5016-5019
- MacGrath J. P. et al. (1991) EMBO J. 10:227-236
- Pickart C. M. et al. (1994) J. Biol. Chem. 269:7115-7123
- Salvat C. et al. (2000) Eur. J. Biochem. 267:3712-3722
- Stephen A. G. et al. (1996) J. Biol. Chem. 271:15608-15614
- Wilkinson K. D. et al. (1990) Biochem. 29:7373-7380
FAQs
No product specific FAQs exist for this product, however you may
View all Proteins and Enzyme FAQsReviews for Recombinant Yeast Ubiquitin Activating Enzyme (UBE1), CF
There are currently no reviews for this product. Be the first to review Recombinant Yeast Ubiquitin Activating Enzyme (UBE1), CF and earn rewards!
Have you used Recombinant Yeast Ubiquitin Activating Enzyme (UBE1), CF?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
