Reelin Antibody (RELN/4498)

Novus Biologicals | Catalog # NBP3-13874

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Mammal

Applications

Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # RELN/4498
View all Reelin Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragment (around aa 1796-2115) of human Reelin protein (Uniprot: P78509)

Reactivity Notes

Rodent

Localization

Cytoplasm

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

387.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14203)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C.

Scientific Data Images for Reelin Antibody (RELN/4498)

Immunohistochemistry-Paraffin: Reelin Antibody (RELN/4498) [NBP3-13874]

Immunohistochemistry-Paraffin: Reelin Antibody (RELN/4498) [NBP3-13874]

Immunohistochemistry-Paraffin: Reelin Antibody (RELN/4498) [NBP3-13874] - Formalin-fixed, paraffin-embedded human kidneystained with Reelin antibody (RELN/4498). Inset: PBS instead of primary antibody; secondary only negative control.

Applications for Reelin Antibody (RELN/4498)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1-2 ug/ml

Immunohistochemistry-Paraffin

1-2 ug/ml

Immunoprecipitation

1-2 ug/100-500 ug protein

Western Blot

1-2 ug/ml
Application Notes
ELISA: For coating, order antibody without BSA

Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Immunoprecipitation: 1-2ug per 100-500ug of total protein (1ml of cell lysate)

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: Reelin

The Reelin gene encodes a large secreted extracellular matrix protein thought to control cell-cell interactions critical for cell positioning and neuronal migration during brain development. This protein may be involved in schizophrenia, autism, bipolar disorde

Alternate Names

Reeler, RELN, RL

Gene Symbol

RELN

UniProt

P78509

Additional Reelin Products

Product Documents for Reelin Antibody (RELN/4498)

Certificate of Analysis

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Product Specific Notices for Reelin Antibody (RELN/4498)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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