RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free

Name: RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-27416
Price: 239 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-27416

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Citations (4)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Flow (Intracellular), Simple Western, CyTOF-ready

Cited:

Immunohistochemistry-Paraffin, Flow Cytometry Control

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 112A1021

Format

Azide and BSA Free

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Product Specifications

Immunogen

This NfkB p65 antibody was raised against a synthetic peptide corresponding to amino acids 526-539 of human NF-kB (p65) protein.

Reactivity Notes

Predicted to react with Bovine, Gorilla, New World Monkey and Porcine.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Scientific Data Images for RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free

Western Blot: RelA/NFkB p65 Antibody (112A1021)Azide and BSA Free [NBP2-27416]

Western Blot: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Lysates of HeLa human cervical epithelial carcinoma cell line and Daudi human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 ug/mL mouse anti-RelA/NFkB p65 monoclonal (NB100-56712, Novus Biologicals), followed by 1:2000 dilution of the appropriate HRP-conjugated secondary antibody, donkey anti-mouse IgG (HAF018). Image using the standard format of this product.

Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free [NBP2-27416]

Immunohistochemistry-Paraffin: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Formalin-fixed, paraffin-embedded ovarian cystadenocarcinoma probed with p65 antibody at 5 ug/ml. Human tissue TMA was used for this test. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Flow Cytometry: RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free [NBP2-27416]

Flow Cytometry: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Intracellular staining of 293 HEK cells using 0.5 ug of p65 antibody. Green histogram represents the isotype control (p65) antibody. this antibody was used for this test, and an anti-mouse IgG PE conjugated secondary antibody.

Western Blot: RelA/NFkB p65 Antibody (112A1021)Azide and BSA Free [NBP2-27416]

Western Blot: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - analysis of p65 using p65 antibody at 2 ug/ml in 30 ug of A) Ramos, B) Daudi, C) HeLa and D) mouse NIH 3T3 cell lysate.

Simple Western: RelA/NFkB p65 Antibody (112A1021)Azide and BSA Free [NBP2-27416]

Simple Western: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Simple Western lane view shows a specific band for NFkB p65 in 1.0 mg/ml of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Simple Western: RelA/NFkB p65 Antibody (112A1021)Azide and BSA Free [NBP2-27416]

Simple Western: RelA/NFkB p65 Antibody (112A1021) - Azide Free [NBP2-27416] - Detection of p65 by Simple Western (JESS) in chemiluminescence in nuclear extract of reconstructed human epidermis lysate (about 600 ug/mL protein concentration). Antibody at 1:20. Simple Western image submitted by a verified customer review. Image using the standard format of this product.

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Applications for RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free

Application

Recommended Usage

Flow (Intracellular)

reported in scientific literature (PMID 15955098)

Flow Cytometry

1ul/1 million cells

Immunohistochemistry-Paraffin

5ug/ml. Use reported in scientific literature (Chang et al (2005))

Western Blot

2-5ug/ml~. Use reported in scientific literature (Wittmann et al (2005))

Application Notes

In Simple Western only 10-15 uL of the recommended dilution is used per data point.

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

Sterile - filtered PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RelA/NFkB p65

NF-kB (nuclear factor kB) regulates the expression of a large number of genes that play critical roles in apoptosis, viral replication, tumorigenesis, various autoimmune diseases and inflammation. The active nuclear form of the NF-kB transcription factor complex is composed of two DNA binding subunits, NF-kB p65 and p50, both of which share extensive N-terminal sequence homology with the v-rel oncogene product. N-terminal regions of p50 and p65 are critical for DNA binding and help determine the DNA-binding specificities of p50 and p65.

Long Name

v-rel Reticuloendotheliosis Viral Oncogene Homolog A

Alternate Names

NFkB p65, NFKB3, p65RelA

Entrez Gene IDs

5970 (Human); 18033 (Mouse); 81736 (Rat)

Gene Symbol

RELA

Additional RelA/NFkB p65 Products

Product Documents for RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free

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Product Specific Notices for RelA/NFkB p65 Antibody (112A1021) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.