RelA/NFkB p65 Antibody (112A1021) - BSA Free

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• RelA/NFkB p65 Antibody (112A1021)

  Name: Brent Stanfield

  Application: Immunoprecipitation

  Sample Tested: A549 human alveolar adenocarcinoma cell line

  Species: Human

  Verified Customer | Posted 02/07/2020

  Acetylated Rela following treatment with MS275 Class 1 HDAC inhibitor

  500 ug of whole cell lysates from cells either left untreated Left or treated with 10 uM MS275 selective Class 1 HDAC inhibitor, Right were immunoprecipitated using 5 ug of RelA/NFkB p65 Antibody 112A1021 conjugated to 50 uL of protein G Dynabeads. Antibody was crosslinked to beads to reduce background using BS3 reagent. following precipitation/washing bound proteins were eluted and ran on a 4-15% gel. Total acetylation was visualized using rabbit anti-acetylated-lysine. bands pictured visualized at ~65 kDA.

  

• RelA/NFkB p65 Antibody (112A1021)

  Name: Agnès Tessier

  Application: Simple Western

  Sample Tested: Human foreskin, adult ski, engineered human skin, keratinocytes, HaCaT cells

  Species: Human

  Verified Customer | Posted 06/17/2019

  Detection of p65 by Simple Western (JESS) in chemiluminescence in nuclear extract of reconstructed human epidermis lysate (about 600 ug/mL protein concentration). Antibody dilution: 1/20

  JESS system

  

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