Respiratory Syncytial Virus Glycoprotein F Antibody (11-6-F9) - BSA Free
Novus Biologicals | Catalog # NBP2-50394
![Western Blot: Respiratory Syncytial Virus Glycoprotein F Antibody (11-6-F9)BSA Free [NBP2-50394]](https://resources.rndsystems.com/images/products/Respiratory-Syncytial-Virus-Glycoprotein-F-Antibody-RSV-11-6-F9-Western-Blot-NBP2-50394-img0001.jpg "Western Blot: Respiratory Syncytial Virus Glycoprotein F Antibody (11-6-F9)BSA Free [NBP2-50394]")
Key Product Details
Species Reactivity
Virus
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Functional
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 11-6-F9
Format
BSA Free
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Product Specifications
Immunogen
Gradient-purified RSF-44 virus (subgroup A) UV inactivated for 20 minutes at 20C
Specificity
Immunoblot using the reduced and unreduced RS virus shows that 11-6-F9 reacts with the non-reduced form of the virus F protein (VP70). It has a plaque reduction neutralization titer of 10 1.3. Induce in vitro antibody-dependent enhancement of macrophage cells.This antibody has a RS virus 90% plaque neutralization titre of 101.3.and it also has RSV enhancing activity (enhancement index range: 2.5-5.3). Gimenez et al. (1996). Gimenez et al. (1996) was the first report showing that a monoclonal antibody specific for the F protein has enhancing activity.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images
Western Blot: Respiratory Syncytial Virus Glycoprotein F Antibody (11-6-F9)BSA Free [NBP2-50394]
Western Blot: Respiratory Syncytial Virus Glycoprotein F Antibody (RSV 11-6-F9) [NBP2-50394] - The antigen was gradient purified RSN-A2 virus (subgroup A). First antibodies: Lane “Hus”: RSV convalescent human sera; Lane “MAb”: 11-6-F9 antibody. The antigen was analysed by electrophoresis using non-reducing conditions (SDS alone) for Lane “MAb” and reducing conditions for Lane “Hus” as described in Gimenez et al. (1986). The identity and molecular weight of the protein target of this antibody was validated by including within the immunoblot assay (as a marker) a convalescent serum sample from a RS virus infected patient. The protein specificities of the antibodies induced in the human convalescent serum is described in Gimenez et al. (1987).Applications
Application
Recommended Usage
ELISA
1:100 - 1:2000
Immunocytochemistry/ Immunofluorescence
1:10 - 1:500
Western Blot
1:100 - 1:2000
Application Notes
Western blot using the reduced and unreduced Respiratory Syncytial Virus shows that 11-6-F9 reacts with the non-reduced form of Respiratory Syncytial Virus Glycoprotein F. Positive control(s): Immunoblot: Ag: gradient-purified Respiratory Syncytial Virus. Indirect immunofluorescence: staining of RSA-2 infected BSC-1 cells.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Respiratory Syncytial Virus Glycoprotein F
Alternate Names
Fusion glycoprotein F0, Fusion glycoprotein F1, Fusion glycoprotein F2
Additional Respiratory Syncytial Virus Glycoprotein F Products
Product Documents
Certificate of Analysis
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Product Specific Notices
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
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