RGS1 Antibody (HL1402) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25635

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Predicted:

Bovine (90%), Rhesus Macaque (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1402 expressed in HEK293

Format

Azide and BSA Free
View all RGS1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RGS1. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RGS1 Antibody (HL1402) - Azide and BSA Free

RGS1 Antibody (HL1402) - Azide and BSA Free

Immunohistochemistry-Paraffin: RGS1 Antibody (HL1402) - Azide and BSA Free [NBP3-25635] -

Immunohistochemistry-Paraffin: RGS1 Antibody (HL1402) - Azide and BSA Free [NBP3-25635] - RGS1 antibody [HL1402] detects RGS1 protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. RGS1 stained by RGS1 antibody [HL1402] (NBP3-25635) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RGS1 Antibody (HL1402) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: RGS1 Antibody (HL1402) - Azide and BSA Free [NBP3-25635] -

Immunocytochemistry/Immunofluorescence: RGS1 Antibody (HL1402) - Azide and BSA Free [NBP3-25635] - RGS1 antibody [HL1402] detects RGS1 protein at cell membrane by immunofluorescent analysis. Sample: Mock and treated THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RGS1 stained by RGS1 antibody [HL1402] (NBP3-25635) diluted at 1:100. Blue: Fluoroshield with DAPI.
RGS1 Antibody (HL1402) - Azide and BSA Free

Western Blot: RGS1 Antibody (HL1402) - Azide and BSA Free [NBP3-25635] -

Western Blot: RGS1 Antibody (HL1402) - Azide and BSA Free [NBP3-25635] - Untreated (-) and treated (+) THP-1 whole cell extract (30 ug) were separated by 12% SDS-PAGE, and the membrane was blotted with RGS1 antibody [HL1402] (NBP3-25635) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for RGS1 Antibody (HL1402) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RGS1

RGS1 encodes a member of the regulator of G protein signalling family. This protein is located on the cytosolic side of the plasma membrane and contains a conserved, 120 amino acid motif called the RGS domain. The protein attenuates the signalling activity of G proteins by binding to activated, GTP bound G alpha subunits and acting as a GTPase activating protein (GAP), increasing the rate of conversion of the GTP to GDP. This hydrolysis allows the G alpha subunits to bind G beta/gamma subunit heterodimers, forming inactive G protein heterotrimers, thereby terminating the signal.

Alternate Names

1R20, 1R20Early response protein 1R20, B-cell activation protein BL34, BL34, Early response protein 1R20, HEL-S-87, IER1, IR20, IR20immediate-early response 1, B-cell specific, regulator of G-protein signaling 1, regulator of G-protein signalling 1, RGS1

Gene Symbol

RGS1

Additional RGS1 Products

Product Documents for RGS1 Antibody (HL1402) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RGS1 Antibody (HL1402) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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