Ribonuclease A Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87804

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Ribonuclease A Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KKFQRQHMDSDSSPSSSSTYCNQMMRRRNMTQGRCKPVNTFVHEPLVDVQNVCFQEKVTCKNGQGNCYKSNSSMHITDCRLTNGSRYPNCAYRTSPKERHIIVACEGSPYVPVHFDASVEDST

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Ribonuclease A Antibody - BSA Free

Western Blot: Ribonuclease A Antibody [NBP1-87804]

Western Blot: Ribonuclease A Antibody [NBP1-87804]

Western Blot: Ribonuclease A Antibody [NBP1-87804] - Analysis in human pancreas tissue.
Immunocytochemistry/ Immunofluorescence: Ribonuclease A Antibody [NBP1-87804]

Immunocytochemistry/ Immunofluorescence: Ribonuclease A Antibody [NBP1-87804]

Immunocytochemistry/Immunofluorescence: Ribonuclease A Antibody [NBP1-87804] - Staining of human cell line RT4 shows localization to nucleoplasm & vesicles.
Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804]

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804]

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining in human pancreas and prostate tissues using NBP1-87804 antibody. Corresponding RNASE1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804]

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804]

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining of human liver shows no positivity in hepatocytes as expected.
Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804]

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804]

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining of human pancreas shows strong cytoplasmic positivity in exocrine glandular cells.
Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804]

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804]

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining of human prostate shows no positivity in glandular cells as expected.
Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804]

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804]

Immunohistochemistry-Paraffin: Ribonuclease A Antibody [NBP1-87804] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for Ribonuclease A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Ribonuclease A

The Ribonuclease A gene encodes a member of the pancreatic-type of secretory ribonucleases, a subset of the ribonuclease A superfamily. The encoded endonuclease cleaves internal phosphodiester RNA bonds on the 3'-side of pyrimidine bases. It prefers poly(C) as a substr

Alternate Names

EC 3.1.27.5, HP-RNase, MGC12408, RIB1, RIB-1, Ribonuclease 1, Ribonuclease A, ribonuclease pancreatic, ribonuclease, RNase A family, 1 (pancreatic), RNase A, RNase UpI-1, RNS1RNase 1

Gene Symbol

RNASE1

Additional Ribonuclease A Products

Product Documents for Ribonuclease A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Ribonuclease A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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