Key Product Details

Validated by

Knockout/Knockdown, Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Rictor Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1650 and the C-terminus (residue 1708) of human Rapamycin-Insensitive Companion of mTOR using the numbering given in TrEMBL entry Q6R327 (GeneID 253260).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

192 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Novus Biologicals Rabbit Rictor Antibody - BSA Free (NB100-612) is a polyclonal antibody validated for use in IHC, WB, ICC/IF and IP. Anti-Rictor Antibody: Cited in 20 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Rictor Antibody - BSA Free

Western Blot: Rictor Antibody [NB100-612]

Western Blot: Rictor Antibody [NB100-612]

Western Blot: Rictor Antibody [NB100-612] - Whole cell lysate (50 ug) from HeLa, HEK293T, and Jurkat cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Knockdown Validated: Rictor Antibody [NB100-612]

Western Blot: Rictor Antibody [NB100-612]

Rictor-Antibody-Western-Blot-NB100-612-img0025.jpg
Immunoprecipitation: Rictor Antibody [NB100-612]

Immunoprecipitation: Rictor Antibody [NB100-612]

Immunoprecipitation: Rictor Antibody [NB100-612] - Detection of human Rictor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-Rictor antibody NB100-612 (lot NB100-612-4) used for IP at 3 ug per reaction. Rictor was also immunoprecipitated by a previous lot of this antibody (lot NB100-612-3) and rabbit anti-Rictor antibody NB100-611. For blotting immunoprecipitated Rictor, NB100-612 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
Western Blot: Rictor Antibody [NB100-612]

Western Blot: Rictor Antibody [NB100-612]

Rictor-Antibody-Western-Blot-NB100-612-img0024.jpg
Immunohistochemistry-Paraffin: Rictor Antibody [NB100-612]

Immunohistochemistry-Paraffin: Rictor Antibody [NB100-612]

Immunohistochemistry-Paraffin: Rictor Antibody [NB100-612] - Human lung carcinoma. Antibody: Affinity purified rabbit anti-Rictor used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Western Blot: Rictor Antibody [NB100-612]

Western Blot: Rictor Antibody [NB100-612]

Western Blot: Rictor Antibody [NB100-612] - Detection of RICTOR on SKOV3 whole cell lysate, treated with PEITC. Image courtesy of product review by Parul Gupta (Texas Tech University Health Sciences Center).
Western Blot: Rictor Antibody [NB100-612]

Western Blot: Rictor Antibody [NB100-612]

Western Blot: Rictor Antibody [NB100-612] - Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-Rictor antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: Rictor Antibody [NB100-612]

Western Blot: Rictor Antibody [NB100-612]

Rictor-Antibody-Western-Blot-NB100-612-img0023.jpg
Rictor Antibody

Western Blot: Rictor Antibody [NB100-612] -

Western Blot: Rictor Antibody [NB100-612] - Truncated Sin1 displaces endogenous Sin1 from mTORC2 in DLD1 colon cancer cellsA. Schematic indicating the domain structure of Sin1 & the constructs used to displace endogenous Sin1 from mTORC2. B. Expression of myc tagged Sin1 constructs can be detected only after induction with Doxycycline (Dox). Cells were treated with 100nM of doxycycline (+) for 72 hours & expressed proteins were detected by immunoblot of whole cell lysates with anti-myc (9E10) antibodies. C. & D. Sin1 constructs incorporate into mTORC2 & displace endogenous Sin1. Constructs were induced for 72 hours prior to immune precipitation. (C) mTORC2 subunits, mTOR & Rictor, only appear in myc immunoprecipitates after induction with doxycycline (Left panels); myc-∆Sin1 cannot be directly detected in precipitates due to secondary antibody cross reaction with precipitating IgG. Right panels indicate unchanging expression levels of Rictor & mTOR in immune precipitation input lysates, which is further quantified from 3 independent experiments E. Endogenous Sin1 & Rictor immunoprecipitates demonstrate displacement of endogenous Sin1 from mTORC2. Following induction, band shifted myc-tagged FL Sin1 can be detected in Sin1 & Rictor precipitates (Left panels). Truncated ∆Sin1 can be detected in Rictor, but not Sin1, immunoprecipitates as the Sin1 antibody epitope is deleted from ∆Sin1. F. Quantification of Sin1 levels detected in Rictor immunoprecipitates indicates the level of endogenous mTORC2 disruption following Sin1 construct induction (data are mean +/- S.D; n = 3). Myc-∆Sin1 displaces >80% of endogenous Sin1 while levels of myc-FL Sin1 associated with Rictor are comparable with endogenous Sin1 levels. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.20086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
Rictor Antibody

Western Blot: Rictor Antibody [NB100-612] -

Western Blot: Rictor Antibody [NB100-612] - Truncated Sin1 displaces endogenous Sin1 from mTORC2 in DLD1 colon cancer cellsA. Schematic indicating the domain structure of Sin1 & the constructs used to displace endogenous Sin1 from mTORC2. B. Expression of myc tagged Sin1 constructs can be detected only after induction with Doxycycline (Dox). Cells were treated with 100nM of doxycycline (+) for 72 hours & expressed proteins were detected by immunoblot of whole cell lysates with anti-myc (9E10) antibodies. C. & D. Sin1 constructs incorporate into mTORC2 & displace endogenous Sin1. Constructs were induced for 72 hours prior to immune precipitation. (C) mTORC2 subunits, mTOR & Rictor, only appear in myc immunoprecipitates after induction with doxycycline (Left panels); myc-∆Sin1 cannot be directly detected in precipitates due to secondary antibody cross reaction with precipitating IgG. Right panels indicate unchanging expression levels of Rictor & mTOR in immune precipitation input lysates, which is further quantified from 3 independent experiments E. Endogenous Sin1 & Rictor immunoprecipitates demonstrate displacement of endogenous Sin1 from mTORC2. Following induction, band shifted myc-tagged FL Sin1 can be detected in Sin1 & Rictor precipitates (Left panels). Truncated ∆Sin1 can be detected in Rictor, but not Sin1, immunoprecipitates as the Sin1 antibody epitope is deleted from ∆Sin1. F. Quantification of Sin1 levels detected in Rictor immunoprecipitates indicates the level of endogenous mTORC2 disruption following Sin1 construct induction (data are mean +/- S.D; n = 3). Myc-∆Sin1 displaces >80% of endogenous Sin1 while levels of myc-FL Sin1 associated with Rictor are comparable with endogenous Sin1 levels. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.20086), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for Rictor Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:250

Immunohistochemistry

1:500-1:2000

Immunohistochemistry-Paraffin

1:500-1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 25378594). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 2 reviews rated 5 using NB100-612 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Rictor

Rictor is a binding partner of mTOR. The rictor mTOR complex modulates the phosphorylation of Protein Kinase C alpha and the actin cytoskeleton.

Long Name

Rapamycin-insensitive Companion of mTOR

Alternate Names

mAVO3, Pianissimo

Entrez Gene IDs

253260 (Human); 78757 (Mouse)

Gene Symbol

RICTOR

UniProt

Q6R327

Additional Rictor Products

Product Documents for Rictor Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for Rictor Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Rictor Antibody - BSA Free

Customer Reviews for Rictor Antibody - BSA Free (2)

5 out of 5
2 Customer Ratings
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Customer Images

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: SKOV3 whole cell lysate
    Species: Human
    Verified Customer | Posted 08/26/2012
    Rictor Antibody - BSA Free NB100-612
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human
    Species: Human
    Verified Customer | Posted 12/09/2011

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