RIG-I Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-76732

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RIG-I Primary Antibodies »

Product Specifications

Immunogen

Antibody was raised against human GST-tagged RIG-1 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RIG-I Antibody - BSA Free

Western Blot: RIG-I AntibodyBSA Free [NBP1-76732]

Western Blot: RIG-I AntibodyBSA Free [NBP1-76732]

Western Blot: RIG-I Antibody [NBP1-76732] - Western blot analysis of RIG-I in Lane 1) A20 cell lysate and Lane 2) Rat heart tissue lysate with RIG-I antibody [NBP1-76732] at 2 ug/mL.
Immunohistochemistry: RIG-I Antibody - BSA Free [NBP1-76732]

Immunohistochemistry: RIG-I Antibody - BSA Free [NBP1-76732]

Immunohistochemistry: RIG-I Antibody [NBP1-76732] - Immunohistochemistry of RIG-I in mouse testis tissue with RIG-I Antibody NBP1-76732 at 1 ug/mL.
RIG-I Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] -

Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] - Immunofluorescence of RIG-I in rat testis tissue with RIG-I Antibody at 20 ug/mL.
RIG-I Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] -

Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] - Immunofluorescence of RIG-I in mouse testis tissue with RIG-I Antibody at 20 ug/mL.
RIG-I Antibody - BSA Free

Immunohistochemistry: RIG-I Antibody - BSA Free [NBP1-76732] -

Immunohistochemistry: RIG-I Antibody - BSA Free [NBP1-76732] - Immunohistochemistry of RIG-I in rat heart tissue with RIG-I Antibody at 10 ug/mL.
RIG-I Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] -

Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] - Immunofluorescence of RIG-I in human testis tissue with RIG-I Antibody at 20 ug/mL.
RIG-I Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] -

Immunocytochemistry/ Immunofluorescence: RIG-I Antibody - BSA Free [NBP1-76732] - Immunofluorescence of RIG-I in MCF7 cells with RIG-I Antibody at 20 ug/mL.
RIG-I Antibody - BSA Free

Western Blot: RIG-I Antibody - BSA Free [NBP1-76732] -

ADAR1 knockdown results in dsRNA increases, type I interferon signaling and pro-inflammatory protein expression. (A) Immunoblots for MDA5 and RIG-I following ADAR1 knockdown. Unpaired t-test; n = 6/condition. (B) Representative images and relative J2 (dsRNA antibody) immunofluorescence signal showing dsRNA accumulation following ADAR1 knockdown. (C) Representative immunofluorescence images showing co-localization of dsRNA (J2) and MDA5 after transfection with ADAR1 siRNA. (D) Summary immunoblot data for ICAM-1, TNF-alpha, pIRF3, pNF-kappa B, and (E) representative images following ADAR1 knockdown. (F) Concentrations of CXCL10 and IFN beta following ADAR1 siRNA knockdown. **p ≤ 0.01, ***p ≤ 0.001, ****p ≤ 0.0001 in unpaired t-test; n = 9/condition. Error bars represent SD. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/38035265), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RIG-I Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

5 ug/ml

Immunohistochemistry-Paraffin

5 ug/ml

Simple Western

1:50

Western Blot

0.5-2 ug/ml
Application Notes
See Simple Western Antibody Database for Simple Western validation: separated by Size, antibody dilution of 1:50

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RIG-I

The innate immune system detects viral infection by recognizing various viral components and triggers antiviral responses. Like the toll-like receptor 3 (TLR3), the cytoplasmic helicase retinoic acid inducible gene protein 1 (RIG-1) recognizes double-stranded (ds) RNA, a molecular pattern associated with viral infection. Unlike TLR3 however, RIG-1 activates the kinases TBK1 and IKKepsilon through the adaptor protein IPS-1. These kinases then phosphorylate the transcription factors IRF-3 and IRF-7 which are essential for the expression of type-I interferons. RIG-1 is required for the production of interferons in response to RNA viruses including paramyxoviruses, influenza virus, and Japanese encephalitis virus.

Long Name

RIG-I

Alternate Names

DDX58, RIGI

Gene Symbol

RIGI

UniProt

O95786

Additional RIG-I Products

Product Documents for RIG-I Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RIG-I Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RIG-I Antibody - BSA Free

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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