RIPK1/RIP1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-87128

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RIPK1/RIP1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: KEYSNENAVVKRMQSLQLDCVAVPSSRSNSATEQPGSLHSSQGLGMGPVEESWFAPSLEHPQEENEPSLQSKLQDEANYHLYGSRMDRQTKQQPRQNVAYNREEERRRRVSHDPFAQQRPYENFQNTEGKGTAYSSAASHGNAV

Reactivity Notes

Reactivity reported in scientific literature (PMID: 23545938)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RIPK1/RIP1 Antibody - BSA Free

Western Blot: RIPK1/RIP1 Antibody [NBP1-87128]

Western Blot: RIPK1/RIP1 Antibody [NBP1-87128]

Western Blot: RIPK1/RIP1 Antibody [NBP1-87128] - Analysis in human cell line HDLM-2.
Immunocytochemistry/ Immunofluorescence: RIPK1/RIP1 Antibody [NBP1-87128]

Immunocytochemistry/ Immunofluorescence: RIPK1/RIP1 Antibody [NBP1-87128]

Immunocytochemistry/Immunofluorescence: RIPK1/RIP1 Antibody [NBP1-87128] - Staining of human cell line A-431 shows localization to cytosol. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128]

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128]

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128] - Staining of human small intestine shows strong cytoplasmic positivity in glandular cells.
Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128]

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128]

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128] - Analysis in human small intestine and skeletal muscle tissues. Corresponding RIPK1/RIP1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128]

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128]

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128] - Staining of human placenta shows moderate cytoplasmic positivity in trophoblastic cells.
Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128]

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128]

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128] - Staining of human prostate shows moderate cytoplasmic positivity in smooth muscle cells.
Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128]

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128]

Immunohistochemistry-Paraffin: RIPK1/RIP1 Antibody [NBP1-87128] - Staining of human skeletal muscle shows no positivity in myocytes as expected.

Applications for RIPK1/RIP1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:50-1:200

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RIPK1/RIP1

RIP (Receptor Interacting Protein) is a 74 kD Ser/Thr kinase which interacts with CD95 (Fas/APO1) receptor and the tumor necrosis factor receptor (TNFR1). It is a cell death domain adapter protein which can bind to the adapter proteins TRADD, RAID (CRADD) and TRAF2. RIP contains an N terminal region with homology to protein kinases, an intermediate domain capable of association with MAPKKK and a C terminal region containing an intracellular death domain motif. RIP activates both p38 MAP Kinase and SAPK families. In vitro, RIP induces apoptosis, as well as SAPK/JNK and NF-kB activation. NF-kB activation through TRADD, TRAF2 and RIP can be triggered also by DR3/APO3 upon activation with APO3/Tweak ligand. DR4 and DR5 also use FADD, TRADD, and RIP in their signal transduction pathways. RIP deficient mice fail to thrive, displaying extensive apoptosis in both lymphoid and adipose tissues and dying at 1-3 days of age. RIP possesses kinase activity as it autophosphorylates itself on serine and threonine residues.

Long Name

Receptor (TNFRSF)-Interacting Serine-Threonine Kinase 1

Alternate Names

RIP, RIP1

Gene Symbol

RIPK1

Additional RIPK1/RIP1 Products

Product Documents for RIPK1/RIP1 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RIPK1/RIP1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RIPK1/RIP1 Antibody - BSA Free

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Protocols

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