RNA Helicase A Antibody

Novus Biologicals | Catalog # NB110-40579

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human, Mouse

Predicted:

Orangutan (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, RNA immunoprecipitation, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RNA Helicase A Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1220 and the C-terminus (residue 1270) of human DEAH (Asp-Glu-Ala-His) Box Polypeptide 9 using the numbering given in entry CAA71668.1 (GeneID 1660).

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24270455).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNA Helicase A Antibody

Western Blot: RNA Helicase A Antibody [NB110-40579]

Western Blot: RNA Helicase A Antibody [NB110-40579]

RNA-Helicase-A-Antibody-Western-Blot-NB110-40579-img0015.jpg
Immunocytochemistry/ Immunofluorescence: RNA Helicase A Antibody [NB110-40579]

Immunocytochemistry/ Immunofluorescence: RNA Helicase A Antibody [NB110-40579]

Immunocytochemistry/Immunofluorescence: RNA Helicase A Antibody [NB110-40579] - Immunocytochemistry of KYSE-30 cells to evaluate colocalization of RNA-helicase A (NDHII, green) and RCC2 (red). Colocalization signal is detected in nucleolus. ICC/IF image submitted by a verified customer review.
Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NB110-40579]

Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NB110-40579]

Immunohistochemistry-Paraffin: RNA Helicase A Antibody [NB110-40579] - FFPE section of human stomach carcinoma. Antibody: Affinity purified rabbit anti-DHX9 used at a dilution of 1:200 (1 ug/mL). Detection: DAB
Western Blot: RNA Helicase A Antibody [NB110-40579]

Western Blot: RNA Helicase A Antibody [NB110-40579]

Western Blot: RNA Helicase A Antibody [NB110-40579] - Detection of Human DHX9 by Western Blot. Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody NB110-40579 used for WB at 0.1 ug/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: RNA Helicase A Antibody [NB110-40579]

Immunoprecipitation: RNA Helicase A Antibody [NB110-40579]

Immunoprecipitation: RNA Helicase A Antibody [NB110-40579] - Detection of human DHX9 by Western blot of immunoprecipitates. Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-DHX9 antibody NB110-40579 (lot NB110-40579-2) used for IP at 6 ug per reaction. DHX9 was also immunoprecipitated by a previous lot of this antibody NB110-40579-1. For blotting immunoprecipitated DHX9, NB110-40579 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Applications for RNA Helicase A Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:500

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100 - 1:500

Immunoprecipitation

2-10 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Use in ICC/IF reported in scientific literature (PMID 25951186). Use in Knockdown reported in scientific literature (PMID: 30111593). Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. This RNA Helicase A Antibody is validated for ICC/IF from a verified customer review.

Reviewed Applications

Read 1 review rated 5 using NB110-40579 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS, 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RNA Helicase A

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein with RNA helicase activity. It may participate in melting of DNA:RNA hybrids, such as those that occur during transcription, and may play a role in X-linked gene expression. It contains 2 copies of a double-stranded RNA-binding domain, a DEXH core domain and an RGG box. The RNA-binding domains and RGG box influence and regulate RNA helicase activity. [provided by RefSeq]

Long Name

ATP-dependent RNA helicase A

Alternate Names

DDX9, DEAH box protein 9, DExH-box helicase 9, DHX9, EC 3.6.4.13, Leukophysin, LKP, NDH II, NDH2

Entrez Gene IDs

1660 (Human)

Gene Symbol

DHX9

UniProt

Q08211 (Human)

Additional RNA Helicase A Products

Product Documents for RNA Helicase A Antibody

Certificate of Analysis

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Product Specific Notices for RNA Helicase A Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RNA Helicase A Antibody

Customer Reviews for RNA Helicase A Antibody (1)

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  • RNA Helicase A Antibody
    Name: Anonymous
    Application: Immunocytochemistry
    Sample Tested: Human Cell lines and 293T lysate
    Species: Human
    Verified Customer | Posted 03/03/2020
    Immunocytochemistry of KYSE-30 cells to evaluate colocalization of RNA-helicase A (NDHII, green) and RCC2 (red). Colocalization signal is detected in nucleo.
    RNA Helicase A Antibody NB110-40579

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