RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Novus Biologicals | Catalog # NBP3-16910

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 10C7U2 expressed in HEK293
View all RNA Polymerase II/POLR2A Primary Antibodies »

Product Specifications

Immunogen

A phospho specific peptide corresponding to residues surrounding S5 of human RNA Polymerase II/POLR2A (P24928).

Modification

p Ser 5

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Western Blot: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Western blot analysis of extracts of various cell lines, using RNA Polymerase II/POLR2A Rabbit mAb (NBP3-16910) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37C for 24 hours or treated by nocodazole (50 ng/mL) at 37C for 20 hours or treated by CIP(20uL/400ul) at 37C for 1 hour.C2C12 cells were treated by CIP(20uL/400ul) at 37C for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 1s.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] -

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Human breast tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] -

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunofluorescence analysis of A-549 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] -

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] -

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] -

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] -

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] -

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunofluorescence analysis of PC-12 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] -

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Rat intestine tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] -

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunofluorescence analysis of NIH/3T3 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] -

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunofluorescence analysis of HeLa cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] -

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] -

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] -

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [NBP3-16910] - Immunohistochemistry analysis of paraffin-embedded Human colon tissue using RNA Polymerase II/POLR2A Rabbit mAb at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] -

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunofluorescence analysis of PC-12 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] -

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunofluorescence analysis of NIH/3T3 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] -

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunofluorescence analysis of HeLa cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] -

Immunocytochemistry/ Immunofluorescence: RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2) [RNA Polymerase II/POLR2A] - Immunofluorescence analysis of A-549 cells using RNA Polymerase II/POLR2A Rabbit mAb at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Applications for RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNA Polymerase II/POLR2A

RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Long Name

DNA-directed RNA Polymerase II Subunit RPB1

Alternate Names

POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2

Gene Symbol

POLR2A

Additional RNA Polymerase II/POLR2A Products

Product Documents for RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

Certificate of Analysis

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Product Specific Notices for RNA Polymerase II/POLR2A [p Ser5] Antibody (10C7U2)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

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Protocols

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