RNA Polymerase II/POLR2A [p Ser2] Antibody

Novus Biologicals | Catalog # NB100-1805

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Yeast

Cited:

Human, Yeast

Predicted:

Drosophila (100%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Single Cell Western

Cited:

Western Blot, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all RNA Polymerase II/POLR2A Primary Antibodies »

Product Specifications

Immunogen

Immunogen for this antibody was a phosphorylated synthetic peptide, which represented a portion of human RNA Polymerase II (GeneID 5430) around serine 2 of the C-terminal repeat YSPTSPS.

Reactivity Notes

Yeast reactivity reported in scientific literature (PMID: 25416796).

Modification

p Ser2

Specificity

RNA Polymerase 2 Phosphospecific [Ser2]

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RNA Polymerase II/POLR2A [p Ser2] Antibody

Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805]

Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805]

Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Human Phospho RNA Polymerase II (S2) by Western Blot. Samples: Whole cell lysate (50 and 15 ug) from HeLa prepared using NETN buffer. Lysate was mock treated (-) or treated with phosphatases (+). Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody NB100-1805 was used for WB at 0.04 ug/ml (upper panel). To examine total RNA Polymerase II, rabbit anti-RNA Polymerase II antibody was used at 1 ug/ml (lower panel). Detection: Chemiluminescence with exposure time of 3 seconds.
Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805]

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805]

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Section of mouse teratoma. Antibody: Affinity purified rabbit anti-PhosphoRNA Polymerase II (S2) used at a dilution of 1:200 (1ug/ml). Detection: DAB
Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805]

Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805]

Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Hela whole cell lysate (50 ug each lane) loaded and probed by anti-RNA polymerase II Antibody. NB100-1805 was pre-incubated without peptide (-), with non-phosphorylated peptide (NP), with phosphorylated S2 peptide, or with phosphorylated S5 peptide.
Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805]

Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805]

Western Blot: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Whole cell lysate (15 and 50 ug) from NIH3T3 cells. Antibody used at 0.08 ug/ml.
Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Analysis of POLR2A in MCF-7 xenograft using anti-POLR2A [p Ser2] antibody. Image from verified customer review.
Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805]

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805]

Immunohistochemistry-Paraffin: RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Human ovarian carcinoma. Antibody: Affinity purified rabbit antiPhospho-RNA Polymerase II (S2) used at a dilution of 1:1000 (0.2ug/ml). Detection: DAB
RNA Polymerase II/POLR2A [p Ser2] Antibody [NB100-1805] - Detection of Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1 mg per IP reaction, 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. immunoprecipitates were either phosphatase treated (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody NB100-1805 used for IP at 5 ug per reaction and another Affinity purified rabbit anti-RNA Polymerase II antibody used for IP at 5 ug per reaction. For blotting immunoprecipitated Phospho RNA Polymerase II (S2) and total RNA Polymerase II, NB100-1805 was used at 0.1 ug/ml (upper panel) and the other antibody was used at 1 ug/ml (lower panel). Detection: Chemiluminescence with an exposure time of 10 seconds (upper) and 75 seconds (lower).

Applications for RNA Polymerase II/POLR2A [p Ser2] Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:1000

Immunohistochemistry-Paraffin

1:200 - 1:1000

Immunoprecipitation

2-10 mg/mg

Single Cell Western

100 ug/mL

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 2 reviews rated 5 using NB100-1805 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: RNA Polymerase II/POLR2A

The RNA polymerase II subunit B1 (RPB1) is the largest subunit of the RNA polymerase II core complex. RNA polymerase II holoenzyme functions to transcribe DNA into messenger RNA in eukaryotes. RPB1 forms the DNA binding domain of the polymerase that forms a cleft that grabs the incoming DNA template to translocate the holoenzyme across the RNA-DNA hybrid. Alternate identifiers of RPB1 include DNA-directed RNA polymerase II subunit RPB1, DNA directed RNA polymerse II subunit A, DNA-directed RNA polymerase II largest subunit, POLR2A, RPO2, POLRA, RPBh1, RPOL2, RpIILS, hsRPB1, hRPB220, and MGC75453.

Long Name

DNA-directed RNA Polymerase II Subunit RPB1

Alternate Names

POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2

Entrez Gene IDs

5430 (Human)

Gene Symbol

POLR2A

UniProt

P24928 (Human)

Additional RNA Polymerase II/POLR2A Products

Product Documents for RNA Polymerase II/POLR2A [p Ser2] Antibody

Certificate of Analysis

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Product Specific Notices for RNA Polymerase II/POLR2A [p Ser2] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Epigenetics

Citations for RNA Polymerase II/POLR2A [p Ser2] Antibody

Customer Reviews for RNA Polymerase II/POLR2A [p Ser2] Antibody (2)

5 out of 5
2 Customer Ratings
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

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  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: MCF7
    Species: Human
    Verified Customer | Posted 10/29/2015
    1:250 POLR2A staining on MCF7 xenograft
    RNA Polymerase II/POLR2A [p Ser2] Antibody NB100-1805
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: 293T cells
    Species: Human
    Verified Customer | Posted 08/26/2014
    WB for RNA Pol II pSer 2 in 293T cells
    RNA Polymerase II/POLR2A [p Ser2] Antibody NB100-1805

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