RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-16906

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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Chromatin Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all RNA Polymerase II/POLR2A Primary Antibodies »

Product Specifications

Immunogen

A phospho specific peptide corresponding to residues surrounding S7 of human RNA Polymerase II/POLR2A. SPSYS

Modification

p Ser7

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

217 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free

Western Blot: RNA Polymerase II/POLR2A [p Ser7] AntibodyAzide and BSA Free [NBP3-16906]

Western Blot: RNA Polymerase II/POLR2A [p Ser7] AntibodyAzide and BSA Free [NBP3-16906]

Western Blot: RNA Polymerase II/POLR2A [p Ser7] Antibody [NBP3-16906] - Western blot analysis of extracts of MCF7 cells, using RNA Polymerase II/POLR2A antibody (NBP3-16906) at 1:1000 dilution.MCF7 cells were treated by Doxorubicin (0.5 uM) at 37C for 24 hours.MCF7 cells were treated by nocodazole (50 ng/ml) at 37C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] -

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Immunohistochemistry analysis of paraffin-embedded Rat brain using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] -

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] -

Immunohistochemistry: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using RNA Polymerase II/POLR2A Rabbit pAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free

Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] -

Chromatin Immunoprecipitation: RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free [NBP3-16906] - Chromatin immunoprecipitation was performed with cross-linked chromatin from HeLa cells, using RNA Polymerase II/POLR2A Rabbit pAb and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram compares the ratio of the immunoprecipitated DNA versus the input.

Applications for RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation

5μg antibody for 10μg-15μg of Chromatin

ELISA

Recommended starting concentration is 1 μg/mL.

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RNA Polymerase II/POLR2A

RNA polymerase II (RNAPII) is an essential component of the RNAP II transcription elongation complex. Recruitment of RNA processing factors to the elongation complex is coordinated by the phosphorylation of Serine-2 and Serine-5 residues within the C-terminal repeat (YSPTSPS) domain of the largest subunit of RNAPII.

Long Name

DNA-directed RNA Polymerase II Subunit RPB1

Alternate Names

POLR2, POLR2A, POLRA, RPB1, RPO2, RPOL2

Gene Symbol

POLR2A

Additional RNA Polymerase II/POLR2A Products

Product Documents for RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for RNA Polymerase II/POLR2A [p Ser7] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Related Research Areas

Epigenetics

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Protocols

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FAQs

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