ROCK2 [p Ser1366] Antibody (HL1071) - Azide and BSA Free

Name: ROCK2 [p Ser1366] Antibody (HL1071) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP3-13711
Price: 489 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-13711

Submit a review

Product Documents

Recombinant Monoclonal Antibody

100% Guarantee

Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1071 expressed in HEK293

Format

Azide and BSA Free

View all ROCK2 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Ser1366 of human ROCK2. The exact sequence is proprietary.

Modification

p Ser 1366

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Centrifuge briefly prior to opening.

Scientific Data Images for ROCK2 [p Ser1366] Antibody (HL1071) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: ROCK2 [p Ser1366] Antibody (HL1071) [NBP3-13711] - ROCK2 (phospho Ser1366) antibody detects ROCK2 (phospho Ser1366) protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: ROCK2 (phospho Ser1366) stained by ROCK2 (phospho Ser1366) antibody (NBP3-13711) diluted at 1:500.

Western Blot: ROCK2 [p Ser1366] Antibody (HL1071) [NBP3-13711] -

Western Blot: ROCK2 [p Ser1366] Antibody (HL1071) [NBP3-13711] - Untreated (-) and treated (+) A431 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 antibody [HL1071] (NBP3-13711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: ROCK2 [p Ser1366] Antibody (HL1071) [NBP3-13711] -

Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with ROCK2 (phospho Ser1366) antibody [HL1071] (NBP3-13711) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. (CIP: Calf-intestinal alkaline phosphatase)

Western Blot: ROCK2 [p Ser1366] Antibody (HL1071) [NBP3-13711] -

Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with primary antibodies diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Upper : ROCK2 (phospho Ser1366) antibody [HL1071] (NBP3-13711)
Lower : ROCK2 antibody

View 1 more images

Applications for ROCK2 [p Ser1366] Antibody (HL1071) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ROCK2

ROCK2 is encoded by this gene is a serine/threonine kinase that regulates cytokinesis, smooth muscle contraction, the formation of actin stress fibers and focal adhesions, and the activation of the c-fos serum response element. This protein, which is an isozyme of ROCK1 is a target for the small GTPase Rho.

Long Name

Rho-associated, Coiled-coil Containing Protein Kinase 2

Alternate Names

ROCK-II, ROKalpha

Gene Symbol

ROCK2

Additional ROCK2 Products

Product Documents for ROCK2 [p Ser1366] Antibody (HL1071) - Azide and BSA Free

Download Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for ROCK2 [p Ser1366] Antibody (HL1071) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.