Roquin Antibody - BSA Free

Novus Biologicals | Catalog # NB100-655

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all Roquin Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residues 500 and 550 of human Ring Finger and CCCH-type finder domains 1 using the numbering given in entry NP_742068.1 (GeneID 149041).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Roquin Antibody - BSA Free

Western Blot: Roquin Antibody [NB100-655]

Western Blot: Roquin Antibody [NB100-655]

Western Blot: Roquin Antibody [NB100-655] - Detection of Mouse Roquin by Western Blot. Samples: Whole cell lysate (50 ug) from TCMK-1, 4T1, and CT26.WT cells. Antibodies: Affinity purified rabbit anti-Roquin antibody NB100-655 used for WB at 2 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: Roquin Antibody [NB100-655]

Western Blot: Roquin Antibody [NB100-655]

Western Blot: Roquin Antibody [NB100-655] - Detection of human Roquin on HeLa whole cell lysate using NB100-655. Roquin was also immunoprecipitated using NB100-656 at 3 ug/mg lysate.

Applications for Roquin Antibody - BSA Free

Application
Recommended Usage

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:500-1:5000
Application Notes
Optimal working dilutions should be determined experimentally by the investigator. Prepare working dilution immediately before use. Use in ICC/IF reported in scientific literature (PMID: 20412057).

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Roquin

Roquin is a member of the RING-type E3 ubiquitin ligase protein family. It was recently found to contribute to autoimmune diseases such as type 1 diabetes and lupus. Research has found that a mutation in roquin causes T-cells to attack their own tissues. Roquin stops T-cells from displaying a stimulatory receptor, ICOS, that may cause the cells to attack normal body tissues. (1)

Alternate Names

KIAA2025RP5-1198E17.5, RING finger and C3H zinc finger protein 1, ring finger and CCCH-type domains 1, RING finger protein 198, RNF198ring finger and CCCH-type zinc finger domains 1, ROQUIN

Entrez Gene IDs

149041 (Human)

Gene Symbol

RC3H1

UniProt

Q5TC82 (Human)

Additional Roquin Products

Product Documents for Roquin Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for Roquin Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Roquin Antibody - BSA Free

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Protocols

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FAQs

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