ROR gamma/RORC/NR1F3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-24503

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse

Cited:

Human

Predicted:

Orangutan (90%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all ROR gamma/RORC/NR1F3 Primary Antibodies »

Product Specifications

Immunogen

A portion of amino acids 1-50 of human ROR gamma/RORC/NR1F3 was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen Porcine, Rat (81%).

Specificity

100% homologous in human isoforms CRA_a).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for ROR gamma/RORC/NR1F3 Antibody - BSA Free

Western Blot: ROR gamma/RORC/NR1F3 AntibodyBSA Free [NBP2-24503]

Western Blot: ROR gamma/RORC/NR1F3 AntibodyBSA Free [NBP2-24503]

Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Total protein from Human and mouse Thymus, Jurkat and U2OS cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ROR Gamma in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.
Immunocytochemistry/ Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody - BSA Free [NBP2-24503]

Immunocytochemistry/ Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody - BSA Free [NBP2-24503]

Immunocytochemistry/Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - U-2 OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ROR gamma overnight at 4C and detected with an anti-rabbit Dylight 488 (Green). Alpha tubulin (DM1A) NB100-690 was used as a co-stain and detected with an anti-mouse Dylight 550 (Red). Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.
Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody - BSA Free [NBP2-24503]

Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody - BSA Free [NBP2-24503]

Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Tissue section of human lymph node using anti- ROR gamma/RORC/NR1F3 antibody. The staining was developed with HRP labeled secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This antibody generated mainly a nuclear staining in a subset of cells and a weak to cytoplasmic staining was also observed in some cells.
Western Blot: ROR gamma/RORC/NR1F3 AntibodyBSA Free [NBP2-24503]

Western Blot: ROR gamma/RORC/NR1F3 AntibodyBSA Free [NBP2-24503]

Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Analysis of human ROR gamma/RORC/NR1F3 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide, 3) in 3T3, and 4) in RAW cell lysate using NBP2-24503.
Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody - BSA Free [NBP2-24503]

Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody - BSA Free [NBP2-24503]

Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Analysis of human testis using NBP2-24503.
ROR gamma/RORC/NR1F3 Antibody - BSA Free

Western Blot: ROR gamma/RORC/NR1F3 Antibody - BSA Free [NBP2-24503] -

CSCC suppressed the NLRP3 Inflammasome Expression (A) Relative quantification of western blot in colonic tissue was performed for protein redistribution of (B) NLRP3 and (C) ROR gamma t. RT-qPCR was performed to analyze the mRNA levels of (D) NLRP3 and (E) IL-1 beta. ELISA was performed to analyze the proinflammatory cytokines (F) IL-1 beta and (G) Caspase-1. (H) Levels of NLRP3 were tested by Immunofluorescence staining in colonic tissue (200 ×, scale bar = 100 μm). Data were presented as mean +/- SD (n = 6). ##p < 0.01, #p < 0.05 vs UC group. **p < 0.01, *p < 0.05 vs control group. ns: no statistical significance. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fphar.2024.1423012/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
ROR gamma/RORC/NR1F3 Antibody - BSA Free

Western Blot: ROR gamma/RORC/NR1F3 Antibody - BSA Free [NBP2-24503] -

Effects of CSCC on STAT3/NLRP3 in RAW264.7 cells (A) Relative quantification of western blot in RAW264.7 cells was performed for protein redistribution of (B) NLRP3, (C) p-STAT3 and (D) ROR gamma t. ELISA was performed to analyze the proinflammatory cytokines (E) TNF-alpha and (F) IL-17A. (G) Levels of NLRP3 were tested by Immunofluorescence staining in colonic tissue (200 ×, scale bar = 100 μm). Data were presented as mean +/- SD (n = 6). ##p < 0.01, #p < 0.05 vs UC group. **p < 0.01, *p < 0.05 vs control group. ns: no statistical significance. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fphar.2024.1423012/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for ROR gamma/RORC/NR1F3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

2.5-10 ug/ml

Immunohistochemistry-Paraffin

10 ug/ml

Immunoprecipitation

reported in scientific literature (PMID 31451788)

Western Blot

0.5-2 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP2-24503 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: ROR gamma/RORC/NR1F3

ROR gamma/RORC/NR1F3, aka RAR Orphan Receptor gamma, is a Nuclear Receptor 1 Thyroid Hormone-Like receptor containing a short A-T rich sequence follow by a single core motif half-site 5'-AGGTCA-3'. RORC is a key regulator of thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. NR1F3 regulates intrinsic transcriptional functions. Unlike other major nuclear receptors, RORC binds as a monomer to hormone response elements and is documented in mouse thymus, adipose, bone, skeletal muscle, liver, kidney, and human skeletal muscle. An N-terminal isoform of ROR gamma, ROR gamma T, inhibits Fas ligand expression and cytokine secretion in immature thymocytes.

Long Name

Retinoic Acid-Related Orphan Receptor gamma

Alternate Names

NR1F3, RORC, RZRG, anti-ROR gamma

Gene Symbol

RORC

UniProt

NP_001009598 (Human)

Additional ROR gamma/RORC/NR1F3 Products

Product Documents for ROR gamma/RORC/NR1F3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for ROR gamma/RORC/NR1F3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for ROR gamma/RORC/NR1F3 Antibody - BSA Free

Customer Reviews for ROR gamma/RORC/NR1F3 Antibody - BSA Free (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Human and hamster melanoma
    Species: Human
    Verified Customer | Posted 10/03/2014
    ROR gamma/RORC/NR1F3 Antibody - BSA Free NBP2-24503

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Protocols

View specific protocols for ROR gamma/RORC/NR1F3 Antibody - BSA Free (NBP2-24503):

Immunocytochemistry Protocol

Culture cells to appropriate density in 35 mm culture dishes or 6-well plates.

1. Remove culture medium and wash the cells briefly in PBS. Add 10% formalin to the dish and fix at room temperature for 10 minutes.
2. Remove the formalin and wash the cells in PBS.
3. Permeablize the cells with 0.1% Triton X100 or other suitable detergent for 10 min.
4. Remove the permeablization buffer and wash three times for 10 minutes each in PBS. Be sure to not let the specimen dry out.
5. To block nonspecific antibody binding, incubate in 10% normal goat serum from 1 hour to overnight at room temperature.
6. Add primary antibody at appropriate dilution and incubate overnight at 4C.
7. Remove primary antibody and replace with PBS. Wash three times for 10 minutes each.
8. Add secondary antibody at appropriate dilution. Incubate for 1 hour at room temperature.
9. Remove secondary antibody and replace with PBS. Wash three times for 10 minutes each.
10. Counter stain DNA with DAPi if required.

Immunohistochemistry-Paraffin Embedded Sections

Antigen Unmasking:
Bring slides to a boil in 10 mM sodium citrate buffer (pH 6.0) then maintain at a sub-boiling temperature for 10 minutes. Cool slides on bench-top for 30 minutes (keep slides in the sodium citrate buffer all the time).

Staining:
1. Wash sections in deionized water three times for 5 minutes each.
2. Wash sections in PBS for 5 minutes.
3. Block each section with 100-400 ul blocking solution (1% BSA in PBS) for 1 hour at room temperature.
4. Remove blocking solution and add 100-400 ul diluted primary antibody. Incubate overnight at 4 C.
5. Remove antibody solution and wash sections in wash buffer three times for 5 minutes each.
6. Add 100-400 ul HRP polymer conjugated secondary antibody. Incubate 30 minutes at room temperature.
7. Wash sections three times in wash buffer for 5 minutes each.
8. Add 100-400 ul DAB substrate to each section and monitor staining closely.
9. As soon as the sections develop, immerse slides in deionized water.
10. Counterstain sections in hematoxylin.
11. Wash sections in deionized water two times for 5 minutes each.
12. Dehydrate sections.
13. Mount coverslips.


Western Blot Protocol

1. Perform SDS-PAGE on samples to be analyzed, loading 10-25 ug of total protein per lane.
2. Transfer proteins to PVDF membrane according to the instructions provided by the manufacturer of the membrane and transfer apparatus.
3. Stain the membrane with Ponceau S (or similar product) to assess transfer success, and mark molecular weight standards where appropriate.
4. Rinse the blot TBS -0.05% Tween 20 (TBST).
5. Block the membrane in 5% Non-fat milk in TBST (blocking buffer) for at least 1 hour.
6. Wash the membrane in TBST three times for 10 minutes each.
7. Dilute primary antibody in blocking buffer and incubate overnight at 4C with gentle rocking.
8. Wash the membrane in TBST three times for 10 minutes each.
9. Incubate the membrane in diluted HRP conjugated secondary antibody in blocking buffer (as per manufacturer's instructions) for 1 hour at room temperature.
10. Wash the blot in TBST three times for 10 minutes each (this step can be repeated as required to reduce background).
11. Apply the detection reagent of choice in accordance with the manufacturers instructions.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for ROR gamma/RORC/NR1F3 Antibody - BSA Free

Showing  1 - 4 of 4 FAQs Showing All

  • Q: Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?

    A: Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.

  • Q: Have you tested NBP2-24503 for cross-reactivity of the T-isoform? If so, can you send any related data you may have?

    A: The NBP2-24503 is made to a region that is mostly missing on the RORgT isoform, so theoretically, there should be minimal cross reaction. But it does not look like we have any concrete testing to show that. Also, it does look like there is a secondary band in our testing that may be this isoform.

  • Q: I would like to know if it was raised against the N terminus region or not, because RORgamma differs from RORgammaT since RORgammaT lacks a N terminus region that RORgamma has.

    A: I can confirm that our product was raised against the N-terminal region (between aa 1-50) of the human RORC sequence found here: Uniprot site.

  • Q: What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?

    A: The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.

  • Q: Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?

    A: Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.

  • Q: Have you tested NBP2-24503 for cross-reactivity of the T-isoform? If so, can you send any related data you may have?

    A: The NBP2-24503 is made to a region that is mostly missing on the RORgT isoform, so theoretically, there should be minimal cross reaction. But it does not look like we have any concrete testing to show that. Also, it does look like there is a secondary band in our testing that may be this isoform.

  • Q: I would like to know if it was raised against the N terminus region or not, because RORgamma differs from RORgammaT since RORgammaT lacks a N terminus region that RORgamma has.

    A: I can confirm that our product was raised against the N-terminal region (between aa 1-50) of the human RORC sequence found here: Uniprot site.

  • Q: What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?

    A: The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.

  • Q: Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?

    A: Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.

  • Q: Have you tested NBP2-24503 for cross-reactivity of the T-isoform? If so, can you send any related data you may have?

    A: The NBP2-24503 is made to a region that is mostly missing on the RORgT isoform, so theoretically, there should be minimal cross reaction. But it does not look like we have any concrete testing to show that. Also, it does look like there is a secondary band in our testing that may be this isoform.

  • Q: I would like to know if it was raised against the N terminus region or not, because RORgamma differs from RORgammaT since RORgammaT lacks a N terminus region that RORgamma has.

    A: I can confirm that our product was raised against the N-terminal region (between aa 1-50) of the human RORC sequence found here: Uniprot site.

  • Q: What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?

    A: The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.

  • Q: Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?

    A: Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.

  • Q: Have you tested NBP2-24503 for cross-reactivity of the T-isoform? If so, can you send any related data you may have?

    A: The NBP2-24503 is made to a region that is mostly missing on the RORgT isoform, so theoretically, there should be minimal cross reaction. But it does not look like we have any concrete testing to show that. Also, it does look like there is a secondary band in our testing that may be this isoform.

  • Q: I would like to know if it was raised against the N terminus region or not, because RORgamma differs from RORgammaT since RORgammaT lacks a N terminus region that RORgamma has.

    A: I can confirm that our product was raised against the N-terminal region (between aa 1-50) of the human RORC sequence found here: Uniprot site.

  • Q: What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?

    A: The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.

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