RPAP2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-13248

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RPAP2 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to the amino acids: RCSRKAAGTKQTSTLKQEDASKRKAELEAAVRKKIEFERKALHIVEQLLEENITEEFLMECGRFITPAHYSDVVDERSIVKLCGYPLCQKKLGI

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RPAP2 Antibody - BSA Free

Western Blot: RPAP2 Antibody [NBP2-13248]

Western Blot: RPAP2 Antibody [NBP2-13248]

Western Blot: RPAP2 Antibody [NBP2-13248] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue
Immunocytochemistry/ Immunofluorescence: RPAP2 Antibody [NBP2-13248]

Immunocytochemistry/ Immunofluorescence: RPAP2 Antibody [NBP2-13248]

Immunocytochemistry/Immunofluorescence: RPAP2 Antibody [NBP2-13248] - Staining of human cell line A-431 shows positivity in nucleoli and cytoplasm.
Immunohistochemistry-Paraffin: RPAP2 Antibody [NBP2-13248]

Immunohistochemistry-Paraffin: RPAP2 Antibody [NBP2-13248]

Immunohistochemistry-Paraffin: RPAP2 Antibody [NBP2-13248] - Staining of human Testis shows strong nuclear and cytoplasmic positivity in cells in seminiferous ducts.
Immunohistochemistry-Paraffin: RPAP2 Antibody [NBP2-13248]

Immunohistochemistry-Paraffin: RPAP2 Antibody [NBP2-13248]

Immunohistochemistry-Paraffin: RPAP2 Antibody [NBP2-13248] - Staining of human Cerebellum shows strong nuclear and cytoplasmic positivity in Purkinje cells.
Immunohistochemistry-Paraffin: RPAP2 Antibody [NBP2-13248]

Immunohistochemistry-Paraffin: RPAP2 Antibody [NBP2-13248]

Immunohistochemistry-Paraffin: RPAP2 Antibody [NBP2-13248] - Staining of human Tonsil shows moderate nuclear and cytoplasmic positivity in squamous epithelial cells.
RPAP2 Antibody - BSA Free Immunohistochemistry: RPAP2 Antibody - BSA Free [NBP2-13248]

Immunohistochemistry: RPAP2 Antibody - BSA Free [NBP2-13248]

Staining of human Kidney shows moderate cytoplasmic positivity in cells in tubules.
RPAP2 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: RPAP2 Antibody - BSA Free [NBP2-13248]

Chromatin Immunoprecipitation-exo-Seq: RPAP2 Antibody - BSA Free [NBP2-13248]

ChIP-Exo-Seq composite graph for Anti-RPAP2 (NBP2-13248) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for RPAP2 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200-1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RPAP2

Forms an interface between the RNA polymerase II enzyme and chaperone/scaffolding protein, suggesting that itis required to connect RNA polymerase II to regulators of protein complex formation

Alternate Names

C1orf82putative RNA polymerase II subunit B1 CTD phosphatase RPAP2, chromosome 1 open reading frame 82, EC 3.1.3.-, FLJ13150, RNA polymerase II associated protein 2, RNA polymerase II-associated protein 2

Gene Symbol

RPAP2

Additional RPAP2 Products

Product Documents for RPAP2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RPAP2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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