RPB9 Antibody - BSA Free

Western Blot: RPB9 Antibody - BSA Free [NBP1-92344]

Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.

Immunohistochemistry: RPB9 Antibody - BSA Free [NBP1-92344]

Staining of human cervix, uterine shows moderate nucleolus positivity in squamous epithelial cells.

Western Blot: RPB9 Antibody - BSA Free [NBP1-92344]

Analysis in human cell line HEK 293.

Chromatin Immunoprecipitation-exo-Seq: RPB9 Antibody - BSA Free [NBP1-92344]

ChIP-Exo-Seq composite graph for Anti-POLR2I (NBP1-92344) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Western Blot: RPB9 Antibody - BSA Free [NBP1-92344] -

Related to Fig 1Western blot quantification of RPB‐9 expression in wild‐type, rpb‐9 (mj261), and rpb‐9 rescue animals. rpb‐9 alleles gk508135 and gk357431 (Thompson et al, 2013) are additional controls that do not show piRNA sensor desilencing.RPB‐9 domain conservations. Alignments of the RPB‐9 protein to its H. sapiens, D. melanogaster and S. cerevisiae homologs. Positive (NCBI BlastP) amino acids are indicated by “+".Comparison of RPB‐9 and the Caenorhabditis elegans TFIIS homolog T24H10.1 with alignment of the common TFIIS C domain. The introduced RPB‐9 Q140* mutation is highlighted in red. Positive (NCBI BlastP) amino acids are indicated by “+".Representative DIC and fluorescence microscopy images of piRNA sensor expression in deficient rescue rpb‑9 (mj261; mjSi89) animals. Scale bar = 20 um.Source data are available online for this figure.
 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33533030), licensed under a CC-BY license. Not internally tested by Novus Biologicals.