RPS10 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-93690

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RPS10 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-165 of human RPS10 (NP_001190174.1). MLMPKKNRIAIYELLFKEGVMVAKKDVHMPKHPELADKNVPNLHVMKAMQSLKSRGYVKEQFAWRHFYWYLTNEGIQYLRDYLHLPPEIVPATLRRSRPETGRPRPKGLEGERPARLTRGEADRDTYRRSAVPPGADKKAEAGAGSATEFQFRGGFGRGRGQPPQ

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPS10 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: RPS10 Antibody - BSA Free [NBP2-93690]

Immunocytochemistry/ Immunofluorescence: RPS10 Antibody - BSA Free [NBP2-93690]

Immunocytochemistry/Immunofluorescence: RPS10 Antibody [NBP2-93690] - Analysis of MCF-7 cells using RPS10. Blue: DAPI for nuclear staining.
RPS10 Antibody - BSA Free

Immunohistochemistry: RPS10 Antibody - BSA Free [NBP2-93690] -

Immunohistochemistry: RPS10 Antibody - BSA Free [NBP2-93690] - Immunohistochemistry analysis of RPS10 in paraffin-embedded human liver tissue using RPS10 Rabbit pAb (A6056) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
RPS10 Antibody - BSA Free

Immunohistochemistry: RPS10 Antibody - BSA Free [NBP2-93690] -

Immunohistochemistry: RPS10 Antibody - BSA Free [NBP2-93690] - Immunohistochemistry analysis of RPS10 in paraffin-embedded mouse brain tissue using RPS10 Rabbit pAb (A6056) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
RPS10 Antibody - BSA Free

Western Blot: RPS10 Antibody - BSA Free [NBP2-93690] -

Western Blot: RPS10 Antibody - BSA Free [NBP2-93690] - Western blot analysis of various lysates, using RPS10 Rabbit pAb (A6056) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.
RPS10 Antibody - BSA Free

Western Blot: RPS10 Antibody - BSA Free [NBP2-93690] -

Western Blot: RPS10 Antibody - BSA Free [NBP2-93690] - Western blot analysis of lysates from Rat thymus, using RPS10 Rabbit pAb (A6056) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
RPS10 Antibody - BSA Free

Immunohistochemistry: RPS10 Antibody - BSA Free [NBP2-93690] -

Immunohistochemistry: RPS10 Antibody - BSA Free [NBP2-93690] - Immunohistochemistry analysis of RPS10 in paraffin-embedded rat colon tissue using RPS10 Rabbit pAb (A6056) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Applications for RPS10 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:100

Immunohistochemistry

1:20-1:200

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPS10

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S10E family of ribosomal proteins. It is located in the cytoplasm. Variable expression of this gene in colorectal cancers compared to adjacent normal tissues has been observed, although no correlation between the level of expression and the severity of the disease has been found. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. (provided by RefSeq)

Alternate Names

DBA9,40S ribosomal protein S10, MGC88819, ribosomal protein S10

Gene Symbol

RPS10

Additional RPS10 Products

Product Documents for RPS10 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for RPS10 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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