RPS18 Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-93632

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all RPS18 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-152 of human RPS18 (NP_072045.1). MSLVIPEKFQHILRVLNTNIDGRRKIAFAITAIKGVGRRYAHVVLRKADIDLTKRAGELTEDEVERVITIMQNPRQYKIPDWFLNRQKDVKDGKYSQVLANGLDNKLREDLERLKKIRAHRGLRHFWGLRVRGQHTKTTGRRGRTVGVSKKK

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RPS18 Antibody - Azide and BSA Free

Western Blot: RPS18 AntibodyAzide and BSA Free [NBP2-93632]

Western Blot: RPS18 AntibodyAzide and BSA Free [NBP2-93632]

Western Blot: RPS18 Antibody [NBP2-93632] - Western blot analysis of extracts of various cell lines, using RPS18 antibody (NBP2-93632) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunohistochemistry-Paraffin: RPS18 Antibody - Azide and BSA Free [NBP2-93632]

Immunohistochemistry-Paraffin: RPS18 Antibody - Azide and BSA Free [NBP2-93632]

Immunohistochemistry-Paraffin: RPS18 Antibody [NBP2-93632] - Rat brain using RPS18 antibody at dilution of 1:100 (40x lens).
Immunocytochemistry/ Immunofluorescence: RPS18 Antibody - Azide and BSA Free [NBP2-93632]

Immunocytochemistry/ Immunofluorescence: RPS18 Antibody - Azide and BSA Free [NBP2-93632]

Immunocytochemistry/Immunofluorescence: RPS18 Antibody [NBP2-93632] - Analysis of HepG2 cells using RPS18 Rabbit pAb at dilution of 1:250 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: RPS18 Antibody - Azide and BSA Free [NBP2-93632]

Immunohistochemistry-Paraffin: RPS18 Antibody - Azide and BSA Free [NBP2-93632]

Immunohistochemistry-Paraffin: RPS18 Antibody [NBP2-93632] - Human thyroid cancer using RPS18 antibody at dilution of 1:100 (40x lens).
Immunohistochemistry-Paraffin: RPS18 Antibody - Azide and BSA Free [NBP2-93632]

Immunohistochemistry-Paraffin: RPS18 Antibody - Azide and BSA Free [NBP2-93632]

Immunohistochemistry-Paraffin: RPS18 Antibody [NBP2-93632] - Mouse kidney using RPS18 antibody at dilution of 1:100 (40x lens).

Applications for RPS18 Antibody - Azide and BSA Free

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:100

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: RPS18

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S13P family of ribosomal proteins. It is located in the cytoplasm. The gene product of the E. coli ortholog (ribosomal protein S13) is involved in the binding of fMet-tRNA, and thus, in the initiation of translation. This gene is an ortholog of mouse Ke3. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. [provided by RefSeq]

Alternate Names

D6S218E, HKE3, KE3, KE-3, Ke-3,40S ribosomal protein S18, MGC117351, MGC126835, MGC126837, rhabdomyosarcoma antigen MU-RMS-40.21, ribosomal protein S18

Gene Symbol

RPS18

Additional RPS18 Products

Product Documents for RPS18 Antibody - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RPS18 Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Citations for RPS18 Antibody - Azide and BSA Free

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Protocols

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