RRBP1 Antibody (5610) - Azide and BSA Free

Novus Biologicals | Catalog # NBP2-43600

Novus Biologicals
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Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Cited:

Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 5610

Format

Azide and BSA Free
View all RRBP1 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the C-terminus region of human RRBP1. The exact sequence is proprietary.

Reactivity Notes

Mouse (81%), Dog (83%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Theoretical MW

152 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RRBP1 Antibody (5610) - Azide and BSA Free

Western Blot: RRBP1 Antibody (5610) [NBP2-43600]

Western Blot: RRBP1 Antibody (5610) [NBP2-43600]

Western Blot: RRBP1 Antibody (5610) [NBP2-43600] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Ribosome binding protein 1 antibody [5610].
RRBP1 Antibody (5610) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: RRBP1 Antibody (5610) [NBP2-43600] -

Ribosome binding protein 1 antibody [GT5610] detects Ribosome binding protein 1 protein at endoplasmic reticulum by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: Ribosome binding protein 1 protein stained by Ribosome binding protein 1 antibody [GT5610] (NBP2-43600) diluted at 1:2000.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for RRBP1 Antibody (5610) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:2000

Proximity Ligation Assay

Reported in scientific literature (PMID:35907632)

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RRBP1

Analysis of cDNA clones indicates that ribosome binding protein 1 may exist in different forms due to removal of tandem repeats, or partial intraexonic splicing of RRBP1. The form presented here is lacking the canine p180 ribosome-binding domain, NQGKKAEGAQ, which is tandemly repeated close to the N-terminus in other forms that haven't been fully characterized. RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome. Alternate splicing results in multiple transcript variants. (provided by RefSeq)

Alternate Names

180 kDa ribosome receptor homolog, DKFZp586A1420, ES/130, ES/130-related protein, ES130, FLJ36146, hES, KIAA1398, MGC157720, MGC157721, ribosome binding protein 1 (dog 180kD homolog), ribosome binding protein 1 homolog 180kDa (dog), Ribosome receptor protein, ribosome-binding protein 1, RRp

Gene Symbol

RRBP1

Additional RRBP1 Products

Product Documents for RRBP1 Antibody (5610) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RRBP1 Antibody (5610) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RRBP1 Antibody (5610) - Azide and BSA Free

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Protocols

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FAQs

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