RRM2 Antibody - Azide Free

Novus Biologicals | Catalog # NBP1-31661

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Zebrafish

Cited:

Human

Predicted:

Bovine (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide Free
View all RRM2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human RRM2. The exact sequence is proprietary.

Localization

Cytoplasm

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

45 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RRM2 Antibody - Azide Free

Immunocytochemistry/ Immunofluorescence: RRM2 Antibody [NBP1-31661]

Immunocytochemistry/ Immunofluorescence: RRM2 Antibody [NBP1-31661]

Immunocytochemistry/Immunofluorescence: RRM2 Antibody [NBP1-31661] - HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RRM2 protein stained by RRM2 antibody [N1C1] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661]

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661]

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse tumor tissues were stained with RRM2 antibody at 1:50 dilution. Image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: RRM2 Antibody [NBP1-31661]

Immunocytochemistry/ Immunofluorescence: RRM2 Antibody [NBP1-31661]

Immunocytochemistry/Immunofluorescence: RRM2 Antibody [NBP1-31661] - HeLa cells were fixed in ice-cold MeOH for 5 min. Green: RRM2 protein stained by RRM2 antibody [N1C1] diluted at 1:1000. Blue: Hoechst 33342 staining. Scale bar = 10 um.
Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661]

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661]

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse muscle. RRM2 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.
Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661]

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661]

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Rat intestine. RRM2 antibody [N1C1] iluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661]

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661]

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - Mouse muscle. RRM2 antibody [N1C1] diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
RRM2 Antibody

Western Blot: RRM2 Antibody [NBP1-31661] -

Western Blot: RRM2 Antibody [NBP1-31661] - Various tissue extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [N1C1] diluted at 1:1000.
RRM2 Antibody

Immunohistochemistry: RRM2 Antibody [NBP1-31661] -

Immunohistochemistry: RRM2 Antibody [NBP1-31661] - Immunohistochemical analysis of paraffin-embedded zebrafish tissue, using RRM2 antibody [N1C1] (NBP1-31661) at 1:300 dilution.
RRM2 Antibody

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] -

Immunohistochemistry-Paraffin: RRM2 Antibody [NBP1-31661] - RRM2 antibody [N1C1] detects RRM2 protein at cytoplasm in rat intestine by immunohistochemical analysis.
Sample: Paraffin-embedded rat intestine.
RRM2 antibody [N1C1] (NBP1-31661) diluted at 1:500.

Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RRM2 Antibody - Azide Free

Western Blot: RRM2 Antibody [NBP1-31661] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RRM2 antibody [N1C1] (NBP1-31661) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody. Corresponding RNA expression data for the same cell lines are based on Human Protein Atlas program.
RRM2 Antibody - Azide Free

Western Blot: RRM2 Antibody - Azide Free [NBP1-31661] -

SARS-CoV-2 causes CHK1 and RRM2 reduction leading to cell cycle progression impairment.A) IF images of infected HNEpC; nuclei were stained with Hoechst. Scale bar, 10 μm. B) Quantification of the percentage of RRM2-expressing cells in infected (SARS-CoV-2 N + ) or not (SARS-CoV-2 N-) HNEpC. C) Immunoblotting of whole cell lysates of Calu-3 infected, or not, with SARS-CoV-2 and analyzed at different time points post-infection. D) Quantification of protein levels shown in C; values are shown as relative to mock-infected samples. E,F) RT–qPCR of CHK1 and RRM2 mRNA expression in infected (V+ ) or mock-infected (V−) Huh7 and Calu-3 cells, respectively. G) Immunoblotting of CDT1 in Huh7 and Calu-3 cells treated as indicated. The experiment was repeated three times with similar results. Source numerical data and unprocessed blots are available in source data.Source data Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36894671), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
RRM2 Antibody - Azide Free

Western Blot: RRM2 Antibody - Azide Free [NBP1-31661] -

SARS-CoV-2 reduces CHK1 and RRM2 levels leading to dNTP shortage.a, Immunoblotting of whole cell lysates of Huh7 infected, or not, with SARS-CoV-2 and analysed at different timepoints post-infection. b, Quantification of protein levels shown in a; values are shown as relative to mock-infected samples. c, Immunofluorescence (IF) images of infected (V+) or mock-infected (V−) Huh7 cells fixed 48 h post-infection; nuclei were stained with DAPI. Scale bar, 10 μm. d, Quantification of CHK1- or RRM2-positive cells shown in c; n = 3 independent experiments. e, dNTP concentration was measured in V− or V+ Huh7 and Calu-3; values are shown as relative to V−. f, Histograms show the percentage of cells in each phase of the cell cycle in V− or V+ Huh7 fixed 48 h post-infection. g, Fraction of V− or V+ Huh7 cells that did not incorporate BrdU (BrdU−) measured by flow cytometry 48 h post-infection. Source numerical data and unprocessed blots are available in source data.Source data Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36894671), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
RRM2 Antibody - Azide Free

Western Blot: RRM2 Antibody - Azide Free [NBP1-31661] -

CHK1 depletion is sufficient to recapitulate the effects of SARS-CoV-2 infection.A) Huh7 transfected with siRNAs against CHK1 mRNA (siCHK1) or siCTRL were stained for CHK1 and propidium iodide (PI) prior to flow cytometry. B) DNA content analysis of V− or V+ Huh7 fixed 48 h post-infection and siCHK1- or siCTRL-transfected Huh7. C) Histograms show the percentage of cells in each phase of the cell cycle upon siCHK1 or siCTRL treatment. D) Bivariate plot showing DNA content (PI) and BrdU incorporation measured by flow cytometry of V− or V+ Huh7 fixed 48 h post-infection and siCHK1- or siCTRL-transfected Huh7. E) Immunoblots of siCHK1- or siCTRL-transfected Huh7. F) Quantification of protein levels shown in E; values are the means +/- s.e.m. of two independent experiments and shown as relative to the siCTRL-transfected sample. G) IF images of Calu-3 transfected with the indicated siRNAs; nuclei were stained with DAPI. Scale bar, 10 μm. H) Quantification of gamma H2AX foci per cell shown in G. I) IF images of cGAS staining in samples as in G; nuclei were stained with DAPI. Scale bar, 10 μm. J) Micronuclei and cGAS+ micronuclei quantifications on total cell number; at least 300 nuclei were scored for each sample. K,L) RT–qPCR for pro-inflammatory cytokines and CHK1 mRNA expression in siCHK1-treated Calu-3 and Huh7 cells, respectively. Values are shown as relative to siCTRL-transfected samples. M) Quantification of the amounts of secreted cytokines and chemokines from siCHK1- or siCTRL-transfected Calu-3 by Bio-Plex multiplex immunoassays. Source numerical data and unprocessed blots are available in source data.Source data Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36894671), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for RRM2 Antibody - Azide Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Simple Western

1:25 - 1:400

Western Blot

Assay dependent
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in ANBL6 Whole cell lysate, separated by Size, antibody dilution of 1:25,1:100,1:400, apparent MW was 45 kDa

Reviewed Applications

Read 1 review rated 5 using NBP1-31661 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Format

Azide Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RRM2

Ribonucleotide reductase catalyzes the formation of deoxyribonucleotides from ribonucleotides. It is composed of 2 non-identical subunits, proteins M1 and M2. Synthesis of M2 is regulated in a cell-cycle dependent fashion.

Long Name

Ribonucleotide Reductase Regulatory Subunit M2

Alternate Names

C2orf48, FLJ25102, RR2M

Entrez Gene IDs

6241 (Human)

Gene Symbol

RRM2

UniProt

P31350 (Human)

Additional RRM2 Products

Product Documents for RRM2 Antibody - Azide Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for RRM2 Antibody - Azide Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RRM2 Antibody - Azide Free

Customer Reviews for RRM2 Antibody - Azide Free (1)

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Customer Images

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  • RRM2 Antibody
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: formalin fixed paraffin embedded mouse tumor tissues
    Species: Mouse
    Verified Customer | Posted 08/11/2017
    Formalin fixed parafin embedded mouse tumor tissues were stained with RRM2 antibody at 1:50 dilution.
    RRM2 Antibody - Azide Free NBP1-31661

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Protocols

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FAQs for RRM2 Antibody - Azide Free

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  • Q: What is the isotype of this antibody?

    A: NBP1-31661 is a polyclonal antibody made in rabbit. It is expected that the majority of the antibodies present will be IgG.

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