RSK2 Antibody (2G10) - Azide and BSA Free

Novus Biologicals | Catalog # H00006197-M01

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 2G10

Format

Azide and BSA Free
View all RSK2 Primary Antibodies »

Product Specifications

Immunogen

RPS6KA3 (NP_004577, 2 a.a. ~ 95 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. PLAQLADPWQKMAVESPSDSAENGQQIMDEPMGEEEINPQTEEVSIKEIAITHHVKEGHEKADPSQFELLKVLGQGSFGKVFLVKKISGSDARQ

Specificity

RPS6KA3 - ribosomal protein S6 kinase, 90kDa, polypeptide 3 (2G10)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for RSK2 Antibody (2G10) - Azide and BSA Free

Western Blot: RSK2 Antibody (2G10) [H00006197-M01]

Western Blot: RSK2 Antibody (2G10) [H00006197-M01]

Western Blot: RSK2 Antibody (2G10) [H00006197-M01] - RPS6KA3 monoclonal antibody (M01), clone 2G10. Analysis of RPS6KA3 expression in HepG2.
Immunocytochemistry/ Immunofluorescence: RSK2 Antibody (2G10) [H00006197-M01]

Immunocytochemistry/ Immunofluorescence: RSK2 Antibody (2G10) [H00006197-M01]

Immunocytochemistry/Immunofluorescence: RSK2 Antibody (2G10) [H00006197-M01] - Analysis of monoclonal antibody to RPS6KA3 on HeLa cell. Antibody concentration 10 ug/ml.
Proximity Ligation Assay: RSK2 Antibody (2G10) [H00006197-M01]

Proximity Ligation Assay: RSK2 Antibody (2G10) [H00006197-M01]

Proximity Ligation Assay: RSK2 Antibody (2G10) [H00006197-M01] - Analysis of protein-protein interactions between MAPK3 and RPS6KA3. HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RPS6KA3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Applications for RSK2 Antibody (2G10) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against cell lysate and recombinant protein for WB. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: RSK2

This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in this gene have been associated with Coffin-Lowry syndrome (CLS). [provided by RefSeq]

Long Name

Ribosomal Protein S6 Kinase II

Alternate Names

ISPK-1, pp90RSK2, RPS6KA3

Entrez Gene IDs

6197 (Human)

Gene Symbol

RPS6KA3

UniProt

NP_004577 (Human)

Additional RSK2 Products

Product Documents for RSK2 Antibody (2G10) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for RSK2 Antibody (2G10) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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