RSK3 [p Ser360, p Thr356] Antibody (HL3299)

Novus Biologicals | Catalog # NBP3-48789

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL3299
View all RSK3 Primary Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding phospho Thr356/Ser360 of human RSK3. The exact sequence is proprietary.

Modification

p Ser360, p Thr356

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit RSK3 [p Ser360, p Thr356] Antibody (HL3299) (NBP3-48789) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for RSK3 [p Ser360, p Thr356] Antibody (HL3299)

RSK3 [p Ser360, p Thr356] Antibody (HL3299)

Western Blot: RSK3 [p Ser360, p Thr356] Antibody (HL3299) [NBP3-48789] -

Untreated (-) and treated (+) NIH-3T3 whole cell extract (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 antibody [HL3299] (NBP3-48789) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RSK3 [p Ser360, p Thr356] Antibody (HL3299)

Western Blot: RSK3 [p Ser360, p Thr356] Antibody (HL3299) [NBP3-48789] -

Untreated (-) and treated (+) HeLa whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with RSK3 antibody [HL3299] (NBP3-48789) diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RSK3 [p Ser360, p Thr356] Antibody (HL3299)

Immunocytochemistry/ Immunofluorescence: RSK3 [p Ser360, p Thr356] Antibody (HL3299) [NBP3-48789] -

RSK3 antibody [HL3299] detects RSK3 protein by immunofluorescent analysis.
Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RSK3 stained by RSK3 antibody [HL3299] (NBP3-48789) diluted at 1:500.
Blue: Fluoroshield with DAPI.

Applications for RSK3 [p Ser360, p Thr356] Antibody (HL3299)

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RSK3

RSK3 encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

Long Name

Ribosomal Protein S6 Kinase III

Alternate Names

RPS6KA2

Gene Symbol

RPS6KA2

Additional RSK3 Products

Product Documents for RSK3 [p Ser360, p Thr356] Antibody (HL3299)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RSK3 [p Ser360, p Thr356] Antibody (HL3299)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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