RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25644

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL2211 expressed in HEK293

Format

Azide and BSA Free
View all RUNX1/CBFA2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant fragmemt of human RUNX1/CBFA2

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free

RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free

Western Blot: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] -

Western Blot: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - Jurkat whole cell and nuclear extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] -

Immunocytochemistry/Immunofluorescence: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - RUNX1 antibody [HL2211] detects RUNX1 protein at nucleus by immunofluorescent analysis. Sample: Jurkat cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: RUNX1 stained by RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:500. Blue: Fluoroshield with DAPI.
RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free

Western Blot: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] -

Western Blot: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free

Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] -

Immunohistochemistry-Paraffin: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - RUNX1 antibody [HL2211] detects RUNX1 protein by immunohistochemical analysis. Sample: Paraffin-embedded mouse tissues. RUNX1 stained by RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:100. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free

Western Blot: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] -

Western Blot: RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free [NBP3-25644] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with RUNX1 antibody [HL2211] (NBP3-25644) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free

Immunocytochemistry/ Immunofluorescence: RUNX1/CBFA2 Antibody (HL2211) [NBP3-25644] -

RUNX1/CBFA2 antibody [HL2211] detects RUNX1/CBFA2 protein at cytoplasm and nucleus by immunofluorescent analysis.
Sample: U87-MG cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: RUNX1/CBFA2 stained by RUNX1/CBFA2 antibody [HL2211] (NBP3-25644) diluted at 1:200.
Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] diluted at 1:1000.

Applications for RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Assay dependent

Immunohistochemistry

Assay dependent

Immunohistochemistry-Paraffin

Assay dependent

Western Blot

Assay dependent

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RUNX1/CBFA2

Core binding factor (CBF) is a heterodimeric transcription factor that binds to the core element of many enhancers and promoters. The protein encoded by this gene represents the alpha subunit of CBF and is thought to be involved in the development of normal hematopoiesis. Chromosomal translocations involving this gene are well-documented and have been associated with several types of leukemia. Three transcript variants encoding different isoforms have been found for this gene.

Long Name

Runt-related Transcription Factor 1

Alternate Names

AML1, CBFA2

Gene Symbol

RUNX1

Additional RUNX1/CBFA2 Products

Product Documents for RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RUNX1/CBFA2 Antibody (HL2211) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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