RUNX3/CBFA3 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-38863

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Cited:

Mouse

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

Immunohistochemistry-Frozen

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all RUNX3/CBFA3 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: DQTKPFPDRFGDLERLRMRVTPSTPSPRGSLSTTSHFSSQPQTPIQGTSELNP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for RUNX3/CBFA3 Antibody - BSA Free

Western Blot: RUNX3/CBFA3 Antibody [NBP2-38863]

Western Blot: RUNX3/CBFA3 Antibody [NBP2-38863]

Western Blot: RUNX3/CBFA3 Antibody [NBP2-38863] - Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human cell line SK-MEL-30
Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody [NBP2-38863]

Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody [NBP2-38863]

Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody [NBP2-38863] - Staining in human lymph node and cerebral cortex tissues using anti-RUNX3 antibody. Corresponding RUNX3 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody [NBP2-38863]

Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody [NBP2-38863]

Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody [NBP2-38863] - Staining of human cerebral cortex shows low expression as expected.
Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody [NBP2-38863]

Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody [NBP2-38863]

Immunohistochemistry-Paraffin: RUNX3/CBFA3 Antibody [NBP2-38863] - Staining of human lymph node shows high expression.

Applications for RUNX3/CBFA3 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: RUNX3/CBFA3

RUNX3 binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, lck, IL-3 and GM-CSF promoters. RUNX3 consists of a heterodimer of an alpha and a beta subunit. The alpha subunit binds DNA as a monomer through the Runt domain. DNA-binding is increased by heterodimerization.

Long Name

Runt-related Transcription Factor 3

Alternate Names

AML2, CBFA3, PEBP2A3, PEBP2AC

Gene Symbol

RUNX3

UniProt

Q13761

Additional RUNX3/CBFA3 Products

Product Documents for RUNX3/CBFA3 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for RUNX3/CBFA3 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for RUNX3/CBFA3 Antibody - BSA Free

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Protocols

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