S100B Antibody (CL2720) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-43801

Novus Biologicals

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL2720

Format

Azide and BSA Free
View all S100B Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P04271, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse S100B Antibody (CL2720) - Azide and BSA Free (NBP2-46626) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for S100B Antibody (CL2720) - Azide and BSA Free

S100B Antibody (CL2720) - Azide and BSA Free Western Blot: S100B Antibody [NBP3-43801]

Western Blot: S100B Antibody [NBP3-43801]

Lane 1: Marker [kDa] 250, 130, 100, 70, 55, 35, 25, 15, 10
Lane 2: Human Cerebral Cortex tissue
S100B Antibody (CL2720) - Azide and BSA Free Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]

Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]

Staining of human cerebellum shows strong nuclear positivity in glial cells.
S100B Antibody (CL2720) - Azide and BSA Free Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]

Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]

Analysis in human cerebral cortex and kidney tissues using NBP3-43801 antibody. Corresponding S100B RNA-seq data are presented for the same tissues.
S100B Antibody (CL2720) - Azide and BSA Free Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]

Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]

Staining of human cerebral cortex shows moderate nuclear positivity in astrocytes.
S100B Antibody (CL2720) - Azide and BSA Free Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]

Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]

Staining of human kidney shows no nuclear positivity as expected.
S100B Antibody (CL2720) - Azide and BSA Free Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]

Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]

Staining of rat brain shows strong positivity in glial cells in the cerebellum.
S100B Antibody (CL2720) - Azide and BSA Free Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]

Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]

Staining of rat brain shows moderate positivity in astrocytes in the hippocampus.
S100B Antibody (CL2720) - Azide and BSA Free Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]

Immunohistochemistry-Paraffin: S100B Antibody [NBP3-43801]

Staining of mouse brain shows moderate positivity in the cerebellum.

Applications for S100B Antibody (CL2720) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: S100B

S100B is a zinc- and calcium-binding protein belonging to the S100 protein family within the EF-hand (helix E-loop-helix F) subgroup (1,2). S100B plays a role in normal central nervous system development, is associated with various neurological diseases such as Alzheimer's and Parkinson's, and it serves as a marker for brain injury (1,2). The S100B protein has a homodimeric structure comprised of two 91-amino acid polypeptide monomers each with a theoretical molecular weight of 10.5 kDa (1,2). Furthermore, each monomer contains two EF-hand regions, four helixes, and a hinge region (2). S100B is predominately expressed in astrocytes, oligodendrocytes, and Schwann cells, but also other cell types including adipocytes (1,3). S100B interacts with toll-like receptor 4 (TLR4) and receptor for advanced glycation end products (RAGE), initiating downstream signaling cascades and transcription factors including JNK/JUN, NFkappaB, and p38, leading to caspase and proinflammatory cytokine production (2). Overall outcomes include neuronal apoptosis, neuroinflammation, and neurodegeneration (1,2). S100B is the most commonly studied astroglia and blood brain barrier biomarker in traumatic brain injury (TBI) (3,4). The serum levels of S100B in patients with TBI is indicative of patient outcomes, where high levels correlate with injury severity and mortality (4). S100B is often in used in combination with additional biomarkers such as glial fibrillary acidic protein (GFAP) and ubiquitin c-terminal hydrolase L1 (UCH-L1) (3,4).

References

1. Yardan, T., Erenler, A. K., Baydin, A., Aydin, K., & Cokluk, C. (2011). Usefulness of S100B protein in neurological disorders. JPMA. The Journal of the Pakistan Medical Association, 61(3), 276-281.

2. Langeh, U., & Singh, S. (2021). Targeting S100B Protein as a Surrogate Biomarker and its Role in Various Neurological Disorders. Current neuropharmacology, 19(2), 265-277. https://doi.org/10.2174/1570159X18666200729100427

3. Thelin, E. P., Nelson, D. W., & Bellander, B. M. (2017). A review of the clinical utility of serum S100B protein levels in the assessment of traumatic brain injury. Acta neurochirurgica, 159(2), 209-225. https://doi.org/10.1007/s00701-016-3046-3

4. Wang, K. K., Yang, Z., Zhu, T., Shi, Y., Rubenstein, R., Tyndall, J. A., & Manley, G. T. (2018). An update on diagnostic and prognostic biomarkers for traumatic brain injury. Expert review of molecular diagnostics, 18(2), 165-180. https://doi.org/10.1080/14737159.2018.1428089

Long Name

S100 Calcium Binding Protein B

Alternate Names

beta (neural), NEF, S100, S100 beta, S100 calcium binding protein B, S100 calcium-binding protein B, S100 calcium-binding protein, beta (neural), S-100 calcium-binding protein, beta chain, 10protein S100-B, S-100 protein beta chain, S-100 protein subunit beta, S100beta

Gene Symbol

S100B

Additional S100B Products

Product Documents for S100B Antibody (CL2720) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for S100B Antibody (CL2720) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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