S1P2/EDG-5/S1PR2 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-26691

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Cited:

Human, Mouse, Rat

Predicted:

Porcine (94%), Xenopus (94%). Backed by our 100% Guarantee.

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all S1P2/EDG-5 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 325-343 of human S1P2/Edg5 was used as immunogen, GenBank no. NP_004221.3.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for S1P2/EDG-5/S1PR2 Antibody - BSA Free

Western Blot: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691]

Western Blot: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691]

Western Blot: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Analysis of S1P2/Edg5 in heart lysate; Lane 1 shows pre-immune sera; Lanes 2, 3 and 4 show this antibody tested on human heart (1 ug/ml), mouse heart (1 ug/ml) and rat heart (2 ug/ml) lysate, respectively.
Immunocytochemistry/ Immunofluorescence: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691]

Immunocytochemistry/ Immunofluorescence: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691]

Immunocytochemistry/Immunofluorescence: S1P2/EDG-5/S1PR2 Antibody [NBP2-26691] - Human PC3 prostate cancer cell line. Primary antibody NBP2-26691 at 1:200, secondary antibody Alexa488, Hoechst 33342 used for nuclear staining. Image from verified customer review.
S1P2/EDG-5/S1PR2 Antibody - BSA Free

Western Blot: S1P2/EDG-5/S1PR2 Antibody - BSA Free [NBP2-26691] -

Increased S1PR2 accumulation in mouse TAD lesions. Representative immunohistochemistry images and the corresponding analysis of mouse S1PR2 (A,C) and S1PR1 (B,D) in TAD lesions (n = 6) and control thoracic aorta (n = 6). Representative immunoblot and the corresponding analysis of mouse S1PR2 (E,F) and S1PR1 (E,G) in TAD lesions (n = 6) and control thoracic aorta (n = 6). Scale: 200 μm, inset: 50 μm. Data are expressed as means +/- SD. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fcvm.2021.748486/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
S1P2/EDG-5/S1PR2 Antibody - BSA Free

Western Blot: S1P2/EDG-5/S1PR2 Antibody - BSA Free [NBP2-26691] -

Increased Sphingosine-1-phosphate receptor 2 (S1PR2) accumulation in human thoracic aortic dissection (TAD) lesions. Representative immunohistochemistry images and the corresponding analysis of human S1PR2 (A,C) and S1PR1 (B,D) in TAD lesions (n = 6) and normal thoracic aorta (n = 3). Representative immunoblot and the corresponding analysis of human S1PR2 (E,F) and S1PR1 (E,G) in TAD lesions (n = 10) and normal thoracic aorta (n = 6). Scale: 200 μm, inset: 50 μm. Data are expressed as means +/- SD. Image collected and cropped by CiteAb from the following open publication (https://www.frontiersin.org/articles/10.3389/fcvm.2021.748486/full), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for S1P2/EDG-5/S1PR2 Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry-Paraffin

reported in scientific literature (PMID 25621765)

Western Blot

1-2 ug/ml

Reviewed Applications

Read 1 review rated 4 using NBP2-26691 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.05% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: S1P2/EDG-5

This EDG5 protein is a member of the G protein-coupled receptors, as well as the EDG family of proteins. This protein participates in sphingosine 1-phosphate-induced cell proliferation, survival, and transcriptional activation.

Long Name

Endothelial Differentiation Gene 5

Alternate Names

AGR16, EDG5, GPCR13, GPCR18, H218, LPB2, S1PR2

Gene Symbol

S1PR2

UniProt

O95136 (Human)

Additional S1P2/EDG-5 Products

Product Documents for S1P2/EDG-5/S1PR2 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for S1P2/EDG-5/S1PR2 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for S1P2/EDG-5/S1PR2 Antibody - BSA Free

Customer Reviews for S1P2/EDG-5/S1PR2 Antibody - BSA Free (1)

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Customer Images

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  • S1P2/EDG-5/S1PR2 Antibody
    Name: Dimitra Kalamida
    Application: Immunofluorescence
    Sample Tested: C42B prostate cell line and PC3 prostate cancer cell line
    Species: Human
    Verified Customer | Posted 07/11/2018
    Alexa 488 secondary used and Hoechst 33342 for nuclear staining
    1:200 dilution of the antibody used
    S1P2/EDG-5/S1PR2 Antibody - BSA Free NBP2-26691

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