SAFB Antibody (6F7) - Azide and BSA Free

Novus Biologicals | Catalog # NB200-332

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 6F7

Format

Azide and BSA Free
View all SAFB Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 345-357 (PEARDSKEDGRKF) of the human SAFB protein.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This is specific for HET SAF B protein.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Theoretical MW

103 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for SAFB Antibody (6F7) - Azide and BSA Free

Western Blot: SAFB Antibody (6F7) [NB200-332]

Western Blot: SAFB Antibody (6F7) [NB200-332]

Western Blot: SAFB Antibody (6F7) [NB200-332] - A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract D. 30 ug HepG2 whole cell lysate/extract 5% SDS-PAGE SAFB antibody dilution: 1:1000 The HRP-conjugated anti-mouse IgG antibody was used to detect the primary antibody.
Immunocytochemistry/ Immunofluorescence: SAFB Antibody (6F7) [NB200-332]

Immunocytochemistry/ Immunofluorescence: SAFB Antibody (6F7) [NB200-332]

Immunocytochemistry/Immunofluorescence: SAFB Antibody (6F7) [NB200-332] - Immunofluorescence analysis of paraformaldehyde-fixed HeLa, antibody at 1:200 dilution.

Applications for SAFB Antibody (6F7) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1-2 ug/ml
Application Notes
ICC/IF, IHC, IP- Assay dependent

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SAFB

Hsp27 ERE-TATA binding protein (HET)/ Scaffold Attachment Factor-B (SAF-B) is a member of a subset of nuclear matrix proteins that mediate the attachment of chromatin to nuclear protein complexes. The calculated molecular weight of HET/SAF-B is 102 kDa (Accession No. U72355), but on SDS PAGE gels HET/SAF-B runs as an approximately 130 kDa protein. This aberrant migration is due to a large number of charged amino acid residues.

Alternate Names

glutathione S-transferase fusion protein, HAP, heat-shock protein (HSP27) estrogen response element and TATA box-bindingprotein, HETDKFZp779C1727, Hsp27 ERE-TATA binding protein, HSP27 ERE-TATA-binding protein, HSP27 estrogen response element-TATA box-binding protein, SAF-B, SAF-B1, SAFB1SAB-B1, scaffold attachment factor B, scaffold attachment factor B1

Entrez Gene IDs

6294 (Human); 224903 (Mouse)

Gene Symbol

SAFB

Additional SAFB Products

Product Documents for SAFB Antibody (6F7) - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for SAFB Antibody (6F7) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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