SALL4 Antibody (CL12183) - BSA Free

Novus Biologicals | Catalog # NBP3-21209

Novus Biologicals
100% Guarantee

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL12183

Format

BSA Free
View all SALL4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a synthetic peptide derived from Q9UJQ4, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Rat (85%).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for SALL4 Antibody (CL12183) - BSA Free

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Analysis in human testis and endometrium tissues using NBP3-21209 antibody. Corresponding SALL4 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Staining of mouse embryo E11 shows moderate to strong nuclear positivity in in a subset of cells in developing hindbrain.
Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Staining of human ovary shows strong nuclear positivity in oocyte.
Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Staining of human endometrium shows no positivity in glandular cells as expected.
Immunocytochemistry/Immunofluorescence: SALL4 Antibody [NBP3-21209] -

Immunocytochemistry/Immunofluorescence: SALL4 Antibody [NBP3-21209] -

Staining of NTERA-2 cells using the Anti-SALL4 monoclonal antibody, showing specific staining in the nucleoplasm and cytosol in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Staining of human cerebellum shows no positivity in Purkinje cells as expected.
Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.
Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Staining of human testis cancer (embryonal carcinoma) shows strong nuclear positivity in tumor cells.
Western Blot: SALL4 Antibody [NBP3-21209] -

Western Blot: SALL4 Antibody [NBP3-21209] -

Analysis in human cell line NTERA-2.
Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Immunohistochemistry-Paraffin: SALL4 Antibody [NBP3-21209] -

Staining of mouse embryo E11 shows strong nuclear positivity in cells in developing testis.

Applications for SALL4 Antibody (CL12183) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:2500 - 1:5000

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: SALL4

Sall4 is encoded by this gene may be a zinc finger transcription factor. Defects in this gene are a cause of Duane-radial ray syndrome (DRRS).

Long Name

Sal-like 4

Alternate Names

DRRS, HSAL4, ZNF797

Gene Symbol

SALL4

Additional SALL4 Products

Product Documents for SALL4 Antibody (CL12183) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for SALL4 Antibody (CL12183) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for SALL4 Antibody (CL12183) - BSA Free

There are currently no reviews for this product. Be the first to review SALL4 Antibody (CL12183) - BSA Free and earn rewards!

Have you used SALL4 Antibody (CL12183) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies