SAM68 Antibody (1A4) - Azide and BSA Free

Novus Biologicals | Catalog # H00010657-M03

Novus Biologicals
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 1A4

Format

Azide and BSA Free
View all SAM68 Primary Antibodies »

Product Specifications

Immunogen

KHDRBS1 (AAH00717, 176 a.a. ~ 275 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. ILGPQGNTIKRLQEETGAKISVLGKGSMRDKAKEEELRKGGDPKYAHLNMDLHVFIEVFGPPCEAYALMAHAMEEVKKFLVPDMMDDICQEQFLELSYLN

Reactivity Notes

Human. Other species not tested.

Specificity

KHDRBS1 (1A4)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Scientific Data Images for SAM68 Antibody (1A4) - Azide and BSA Free

Western Blot: SAM68 Antibody (1A4) [H00010657-M03]

Western Blot: SAM68 Antibody (1A4) [H00010657-M03]

Western Blot: SAM68 Antibody (1A4) [H00010657-M03] - Analysis of KHDRBS1 expression in A-431 (Cat # L015V1).
Immunocytochemistry/ Immunofluorescence: SAM68 Antibody (1A4) [H00010657-M03]

Immunocytochemistry/ Immunofluorescence: SAM68 Antibody (1A4) [H00010657-M03]

Immunocytochemistry/Immunofluorescence: SAM68 Antibody (1A4) [H00010657-M03] - Analysis of monoclonal antibody to KHDRBS1 on A-431 cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: SAM68 Antibody (1A4) [H00010657-M03]

Immunohistochemistry-Paraffin: SAM68 Antibody (1A4) [H00010657-M03]

Immunohistochemistry-Paraffin: SAM68 Antibody (1A4) [H00010657-M03] - Analysis of monoclonal antibody to KHDRBS1 on formalin-fixed paraffin-embedded human liver. Antibody concentration 3 ug/ml
Western Blot: SAM68 Antibody (1A4) [H00010657-M03]

Western Blot: SAM68 Antibody (1A4) [H00010657-M03]

Western Blot: SAM68 Antibody (1A4) [H00010657-M03] - Analysis of KHDRBS1 expression in transfected 293T cell line by KHDRBS1 monoclonal antibody (M03), clone 1A4. Lane 1: KHDRBS1 transfected lysatE (41 KDa). Lane 2: Non-transfected lysate.
ELISA: SAM68 Antibody (1A4) [H00010657-M03]

ELISA: SAM68 Antibody (1A4) [H00010657-M03]

ELISA: SAM68 Antibody (1A4) [H00010657-M03] - Detection limit for recombinant GST tagged KHDRBS1 is approximately 0.03ng/ml as a capture antibody.

Applications for SAM68 Antibody (1A4) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: SAM68

Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export Function: Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase

Long Name

Src-associated in Mitosis 68 kDa Protein

Alternate Names

KHDRBS1, p62, p68

Entrez Gene IDs

10657 (Human)

Gene Symbol

KHDRBS1

OMIM

602489 (Human)

UniProt

AAH00717 (Human)

Additional SAM68 Products

Product Documents for SAM68 Antibody (1A4) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for SAM68 Antibody (1A4) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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