SAM68 Antibody

Novus Biologicals | Catalog # NBP1-19151

Novus Biologicals
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Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human, Bovine, Mammal

Cited:

Human, Bovine, Mammal

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all SAM68 Primary Antibodies »

Product Specifications

Immunogen

The epitope this antibody maps to a region between residue 1 and 50 of human SAM68 (KH domain containing, RNA binding, signal transduction associated 1) NP_006550.1 (GeneID 10657).

Reactivity Notes

Mammal reactivity reported in scientific literature (PMID: 27180817). Bovine reactivity reported in scientific literature (PMID: 22280896).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for SAM68 Antibody

Western Blot: SAM68 Antibody [NBP1-19151]

Western Blot: SAM68 Antibody [NBP1-19151]

Western Blot: SAM68 Antibody [NBP1-19151] - Whole cell lysate from HeLa cells. SAM68 was also immunoprecipitated by rabbit anti-SAM68 antibodies NBP1-19152 and NBP1-19153.
Immunohistochemistry-Paraffin: SAM68 Antibody [NBP1-19151]

Immunohistochemistry-Paraffin: SAM68 Antibody [NBP1-19151]

Immunohistochemistry-Paraffin: SAM68 Antibody [NBP1-19151] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti- SAM68 used at a dilution of 1:1,000 (0.2 ug/ml). Detection: DAB

Applications for SAM68 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Reported in scientific literature (PMID: 22280896)

Immunohistochemistry

1:500 - 1:2000

Immunohistochemistry-Paraffin

1:500 - 1:2000

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Reviewed Applications

Read 1 review rated 3 using NBP1-19151 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: SAM68

Recruited and tyrosine phosphorylated by several receptor systems, for example the T-cell, leptin and insulin receptors. Once phosphorylated, functions as an adapter protein in signal transduction cascades by binding to SH2 and SH3 domain-containing proteins. Role in G2-M progression in the cell cycle. Represses CBP-dependent transcriptional activation apparently by competing with other nuclear factors for binding to CBP. Also acts as a putative regulator of mRNA stability and/or translation rates and mediates mRNA nuclear export Function: Isoform 3, which is expressed in growth-arrested cells only, inhibits S phase

Long Name

Src-associated in Mitosis 68 kDa Protein

Alternate Names

KHDRBS1, p62, p68

Entrez Gene IDs

10657 (Human)

Gene Symbol

KHDRBS1

UniProt

Q07666

Additional SAM68 Products

Product Documents for SAM68 Antibody

Certificate of Analysis

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Product Specific Notices for SAM68 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for SAM68 Antibody (1)

3 out of 5
1 Customer Rating
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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: Hela whole cell lysate
    Species: Human
    Verified Customer | Posted 10/16/2015
    Hela cells lysate 25 ug protein loaded on SDS gel (4-12%) used Bis-Tris gel for electrophoresis. Major bands ~52 and 58 kDa
    SAM68 Antibody NBP1-19151

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