SAMM50 Antibody - BSA Free
Novus Biologicals | Catalog # NBP1-84509
![Immunocytochemistry/ Immunofluorescence: SAMM50 Antibody [NBP1-84509]](https://resources.rndsystems.com/images/products/SAMM50-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84509-img0012.jpg "Immunocytochemistry/ Immunofluorescence: SAMM50 Antibody [NBP1-84509]")
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Key Product Details
Validated by
Orthogonal Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western
Cited:
Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
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Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HARSLEPLPSSGPDFGGLGEEAEFVEVEPEAKQEILENKDVVVQHVHFDGLGRTKDDIIICEIGDVFKAKNLIEVMRKSHEAREKLLRLGIFRQVDVLID
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for SAMM50 Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: SAMM50 Antibody [NBP1-84509]
Immunocytochemistry/Immunofluorescence: SAMM50 Antibody [NBP1-84509] - Staining of human cell line A-431 shows localization to mitochondria. Antibody staining is shown in green.![Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]](https://resources.rndsystems.com/images/products/SAMM50-Antibody-Immunohistochemistry-Paraffin-NBP1-84509-img0024.jpg "Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]")
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human parathyroid gland shows very strong granular cytoplasmic positivity in glandular cells.![Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]](https://resources.rndsystems.com/images/products/SAMM50-Antibody-Immunohistochemistry-Paraffin-NBP1-84509-img0019.jpg "Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]")
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human small intestine shows strong cytoplasmic granular positivity in glandular cells.![Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]](https://resources.rndsystems.com/images/products/SAMM50-Antibody-Immunohistochemistry-Paraffin-NBP1-84509-img0021.jpg "Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]")
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human kidney shows very strong cytoplasmic granular positivity in cells in tubules.![Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]](https://resources.rndsystems.com/images/products/SAMM50-Antibody-Immunohistochemistry-Paraffin-NBP1-84509-img0022.jpg "Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]")
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509]
Immunohistochemistry-Paraffin: SAMM50 Antibody [NBP1-84509] - Staining of human pancreas shows moderate cytoplasmic granular positivity in exocrine glandular cells.![Simple Western: SAMM50 Antibody [NBP1-84509]](https://resources.rndsystems.com/images/products/SAMM50-Antibody-Simple-Western-NBP1-84509-img0008.jpg "Simple Western: SAMM50 Antibody [NBP1-84509]")
Simple Western: SAMM50 Antibody [NBP1-84509]
Simple Western: SAMM50 Antibody [NBP1-84509] - Simple Western lane view shows a specific band for SAMM50 in 0.2 mg/ml of RT-4 (Left) and U-251MG (Right) lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.![Simple Western: SAMM50 Antibody [NBP1-84509]](https://resources.rndsystems.com/images/products/SAMM50-Antibody-Simple-Western-NBP1-84509-img0009.jpg "Simple Western: SAMM50 Antibody [NBP1-84509]")
Simple Western: SAMM50 Antibody [NBP1-84509]
Simple Western: SAMM50 Antibody [NBP1-84509] - Electropherogram image(s) of corresponding Simple Western lane view. SAMM50 antibody was used at 1:4 dilution on RT-4 and U-251MG lysate(s).![SAMM50 Antibody - BSA Free Immunohistochemistry-Paraffin: SAMM50 Antibody - BSA Free [NBP1-84509]](https://resources.rndsystems.com/images/products/nbp1-84509_rabbit-polyclonal-samm50-antibody-10420251538698.jpg "Immunohistochemistry-Paraffin: SAMM50 Antibody - BSA Free [NBP1-84509]")
Immunohistochemistry-Paraffin: SAMM50 Antibody - BSA Free [NBP1-84509]
Staining of human testis shows strong cytoplasmic granular positivity in Leydig cells.![SAMM50 Antibody - BSA Free Western Blot: SAMM50 Antibody - BSA Free [NBP1-84509]](https://resources.rndsystems.com/images/products/nbp1-84509_rabbit-polyclonal-samm50-antibody-104202515371734.jpg "Western Blot: SAMM50 Antibody - BSA Free [NBP1-84509]")
Western Blot: SAMM50 Antibody - BSA Free [NBP1-84509]
Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.![SAMM50 Antibody - BSA Free Western Blot: SAMM50 Antibody - BSA Free [NBP1-84509]](https://resources.rndsystems.com/images/products/nbp1-84509_rabbit-polyclonal-samm50-antibody-10420251538662.jpg "Western Blot: SAMM50 Antibody - BSA Free [NBP1-84509]")
Western Blot: SAMM50 Antibody - BSA Free [NBP1-84509]
Analysis in human cell line HL-60.![SAMM50 Antibody - BSA Free Immunohistochemistry-Paraffin: SAMM50 Antibody - BSA Free [NBP1-84509]](https://resources.rndsystems.com/images/products/nbp1-84509_rabbit-polyclonal-samm50-antibody-104202515403113.jpg "Immunohistochemistry-Paraffin: SAMM50 Antibody - BSA Free [NBP1-84509]")
Applications for SAMM50 Antibody - BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Simple Western
1:4
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin, HIER pH6 retrieval is recommended. See Simple Western Antibody Database for Simple Western validation: Tested in RT-4 and U-251MG, separated by Size, antibody dilution of 1:4, apparent MW was 56 kDa
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Format
BSA Free
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: SAMM50
Alternate Names
CGI-51, FLJ42905, FLJ99036, OMP85, SAM50, sorting and assembly machinery 50kDa, sorting and assembly machinery component 50 homolog, sorting and assembly machinery component 50 homolog (S. cerevisiae), TOB55, Transformation-related gene 3 protein, transformation-related protein 3, TRG-3FLJ35825, YNL026W
Gene Symbol
SAMM50
Additional SAMM50 Products
Product Documents for SAMM50 Antibody - BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for SAMM50 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Citations for SAMM50 Antibody - BSA Free
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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