Sample Data: Mouse Cytokine Array, Panel A

Figure 1
Figure 1. R&D Systems Mouse Cytokine Array, Panel A (Catalog # ARY006) utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. Mouse splenocytes treated with 100 ng/mL LPS for 20 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Mouse Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane.


Location Analyte Location Analyte
A1 Positive Control C8 IL-12p70
A2 Blank C9 IL-16
A3 Blank C10 IL-17
A4 Blank C11 IL-23
A5 Blank C12 IL-27
A6 Blank D1 IP-10/CXCL10
A7 Blank D2 I-TAC/CXCL11
A8 Blank D3 KC
A9 Blank D4 M-CSF
A10 Blank D5 JE/MCP-1/CCL2
A11 Blank D6 MCP-5/CCL12
A12 Positive Control D7 MIG/CXCL9
B1 BLC/BCA-1/CXCL13 D8 MIP-1 alpha/CCL3
B2 C5a D9 MIP-1 beta/CCL4
B3 G-CSF D10 MIP-2
B5 I-309/CCL1 D12 SDF-1/CXCL12
B6 Eotaxin/CCL11 E1 TARC/CCL17
B8 IFN-gamma E3 TNF-alpha
B9 IL-1 alpha E4 TREM-1
B10 IL-1 beta E5 Blank
B11 IL-1ra E6 Blank
B12 IL-2 E7 Blank
C1 IL-3 E8 Blank
C2 IL-4 E9 Blank
C3 IL-5 E10 Blank
C4 IL-6 E11 Blank
C5 IL-7 E12 Blank
C6 IL-10 F1 Positive Control
C7 IL-13