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| Figure 1. R&D Systems Mouse Cytokine Array, Panel A (Catalog # ARY006) utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. Mouse splenocytes treated with 100 ng/mL LPS for 20 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Mouse Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane. |
| Location | Analyte | Location | Analyte |
| A1 | Positive Control | C8 | IL-12p70 |
| A2 | Blank | C9 | IL-16 |
| A3 | Blank | C10 | IL-17 |
| A4 | Blank | C11 | IL-23 |
| A5 | Blank | C12 | IL-27 |
| A6 | Blank | D1 | IP-10/CXCL10 |
| A7 | Blank | D2 | I-TAC/CXCL11 |
| A8 | Blank | D3 | KC |
| A9 | Blank | D4 | M-CSF |
| A10 | Blank | D5 | JE/MCP-1/CCL2 |
| A11 | Blank | D6 | MCP-5/CCL12 |
| A12 | Positive Control | D7 | MIG/CXCL9 |
| B1 | BLC/BCA-1/CXCL13 | D8 | MIP-1 alpha/CCL3 |
| B2 | C5a | D9 | MIP-1 beta/CCL4 |
| B3 | G-CSF | D10 | MIP-2 |
| B4 | GM-CSF | D11 | RANTES/CCL5 |
| B5 | I-309/CCL1 | D12 | SDF-1/CXCL12 |
| B6 | Eotaxin/CCL11 | E1 | TARC/CCL17 |
| B7 | ICAM-1 | E2 | TIMP-1 |
| B8 | IFN-gamma | E3 | TNF-alpha |
| B9 | IL-1 alpha | E4 | TREM-1 |
| B10 | IL-1 beta | E5 | Blank |
| B11 | IL-1ra | E6 | Blank |
| B12 | IL-2 | E7 | Blank |
| C1 | IL-3 | E8 | Blank |
| C2 | IL-4 | E9 | Blank |
| C3 | IL-5 | E10 | Blank |
| C4 | IL-6 | E11 | Blank |
| C5 | IL-7 | E12 | Blank |
| C6 | IL-10 | F1 | Positive Control |
| C7 | IL-13 |