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Proteome Profiler Mouse Cytokine Array Kit, Panel A

ARY006 264 Citations 31 Reviews Datasheet / COA / SDS
Contains 4 membranes - each spotted in duplicate with 40 different cytokine antibodies
Catalog # Availability Size / Price Qty
ARY006
The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample.
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Citations (264)
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Reviews (31)
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Proteome Profiler Mouse Cytokine Array Kit, Panel A Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.

Troubleshooting Guide

Key Benefits

  • Detects 40 mouse cytokines, chemokines, and acute phase proteins simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR and chemiluminescence detection

Principle of the Assay

The Proteome Profiler Mouse Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Mouse Cytokine Array, Panel A nitrocellulose membranes spotted with 40 different antibodies to mouse cytokines, chemokines, and acute phase proteins
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Mouse Cytokine Array, Panel A
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.  

 
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
CXCL13/BLC/BCA-1 IL-5 M-CSF
C5a IL-6 CCL2/JE/MCP-1
G-CSF IL-7 CCL12/MCP-5
GM-CSF IL-10 CXCL9/MIG
CCL1/I-309 IL-12 p70 CCL3/MIP-1 alpha
CCL11/Eotaxin IL-13 CCL4/MIP-1 beta
ICAM-1 IL-16 CXCL2/MIP-2
IFN-gamma IL-17 CCL5/RANTES
IL-1 alpha/IL-1F1 IL-23 CXCL12/SDF-1
IL-1 beta/IL-1F2 IL-27 CCL17/TARC
IL-1ra/IL-1F3 CXCL10/IP-10 TIMP-1
IL-2 CXCL11/I-TAC TNF-alpha
IL-3 CXCL1/KC TREM-1
IL-4    

 

Assays for Analytes represented in the Mouse Cytokine Array Kit, Panel A

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
CXCL13/BLC/BCA-1 MCX130 DY470
C5a   DY2150
G-CSF MCS00 DY414
GM-CSF MGM00 DY415
CCL1/I-309/TCA-3   DY845
CCL11/Eotaxin MME00 DY420
ICAM-1 MIC100 DY796
IFN-gamma MIF00 DY485
IL-1 alpha MLA00 DY400
IL-1 beta MLB00B DY401
IL-1ra MRA00 DY480
IL-2 M2000 DY402
IL-3 M3000 DY403
IL-4 M4000B DY404
IL-5 M5000 DY405
IL-6 M6000B DY406
IL-7 M7000 DY407
IL-10 M1000b DY417
IL-12 p70 M1270 DY419
IL-13 M1300CB DY413
IL-16    
IL-17 M1700 DY421
IL-23 M2300 DY1887
IL-27 M2728  
CXCL10/IP-10/CRG-2 MCX100 DY466
CXCL11/I-TAC   DY572
CXCL1/KC MKC00B DY453
M-CSF MMC00 DY416
CCL2/MCP-1/JE MJE00 DY479
CCL12/MCP-5 MCC120 DY428
CXCL9/MIG MCX900 DY492
CCL3/MIP-1 alpha MMA00 DY450
CCL4/MIP-1 beta MMB00 DY451
CXCL2/MIP-2 MM200 DY452
CCL5/RANTES MMR00 DY478
CXCL12/SDF-1 MCX120 DY460
CCL17/TARC   DY529
TIMP-1 MTM100 DY980
TNF-alpha MTA00 DY410
TREM-1 MTRM10 DY1187

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Mouse

Product Datasheets

Product Datasheet
COA
SDS

Data Example

The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. View Larger

The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. Mouse splenocytes treated with 100 ng/mL LPS for 20 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Mouse Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane.

The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates. View Larger

The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates. (A and B.) Samples of mouse serum from two different vendors. 200 µL of serum was used for each array shown. (C. and D.) Lysates of mouse lung tissue and mouse stomach tissue. 200 µg of lysate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. View Larger

The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. (A. and B.) Lysates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µg of lysate was used for each array shown. (C. and D.) Supernates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

Citations for Proteome Profiler Mouse Cytokine Array Kit, Panel A

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

264 Citations: Showing 1 - 10
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  1. Recombinant annexin A2 inhibits peripheral leukocyte activation and brain infiltration after traumatic brain injury
    Authors: N Liu, J Han, Y Li, Y Jiang, SX Shi, J Lok, M Whalen, AS Dumont, X Wang
    Journal of Neuroinflammation, 2021;18(1):173.  2021
  2. JAK/STAT inhibitor therapy partially rescues the lipodystrophic autoimmune phenotype in Clec16a KO mice
    Authors: R Pandey, M Bakay, BP Strenkowsk, HS Hain, H Hakonarson
    Scientific Reports, 2021;11(1):7372.  2021
  3. Persistent Airway Hyperresponsiveness Following Recovery from Infection with Pneumonia Virus of Mice
    Authors: AR Limkar, CM Percopo, JL Redes, KM Druey, HF Rosenberg
    Viruses, 2021;13(5):.  2021
  4. Conditioned secretome of adipose-derived stem cells improves dextran sulfate sodium-induced colitis in mice
    Authors: S Lee, J Heo, EK Ahn, JH Kim, YH Kim, HK Chang, SJ Lee, J Kim, SJ Park
    World Journal of Gastroenterology, 2021;27(23):3342-3356.  2021
  5. Vaccine delivery alerts innate immune systems for more immunogenic vaccination
    Authors: Z Li, Y Cao, Y Li, Y Zhao, X Chen
    JCI Insight, 2021;0(0):.  2021
  6. Differential expression of Triggering Receptor Expressed on Myeloid cells 2 (Trem2) in tissue eosinophils
    Authors: AC Sek, CM Percopo, AK Boddapati, M Ma, WE Geslewitz, JO Krumholz, JB Lack, HF Rosenberg
    Journal of leukocyte biology, 2021;0(0):.  2021
  7. Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
    Authors: LG Lima, S Ham, H Shin, EPZ Chai, ESH Lek, RJ Lobb, AF Müller, S Mathivanan, B Yeo, Y Choi, BS Parker, A Möller
    Nature Communications, 2021;12(1):3543.  2021
  8. The Protective Effect of Anthocyanins Extracted from Aronia Melanocarpa Berry in Renal Ischemia-Reperfusion Injury in Mice
    Authors: L Li, J Li, H Xu, F Zhu, Z Li, H Lu, J Zhang, Z Yang, Y Liu
    Mediators of Inflammation, 2021;2021(0):7372893.  2021
  9. Moderately pathogenic maternal influenza A virus infection disrupts placental integrity but spares the fetal brain
    Authors: AM Antonson, AD Kenney, HJ Chen, KN Corps, JS Yount, TL Gur
    Brain, Behavior, and Immunity, 2021;0(0):.  2021
  10. KCNK3 Mutation Causes Altered Immune Function in Pulmonary Arterial Hypertension Patients and Mouse Models
    Authors: JD West, ED Austin, EM Rizzi, L Yan, H Tanjore, AL Crabtree, CS Moore, G Muthian, EJ Carrier, DA Jacobson, R Hamid, PL Kendall, S Majka, A Rathinasab
    International Journal of Molecular Sciences, 2021;22(9):.  2021
  11. Three-dimensional culture method enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine chronic colitis model
    Authors: EM Song, YH Joo, AR Choe, Y Park, CH Tae, JT Hong, CM Moon, SE Kim, HK Jung, KN Shim, KA Cho, I Jo, SA Jung
    Scientific Reports, 2021;11(1):19589.  2021
  12. Distinct transcription factor networks control neutrophil-driven inflammation
    Authors: TE Khoyratty, Z Ai, I Ballestero, HL Eames, S Mathie, S Martín-Sal, L Wang, A Hemmings, N Willemsen, V von Werz, A Zehrer, B Walzog, E van Grinsv, A Hidalgo, IA Udalova
    Nature Immunology, 2021;0(0):.  2021
  13. Chamaecyparis obtusa (Siebold & Zucc.) Endl. leaf extracts prevent inflammatory responses via inhibition of the JAK/STAT axis in RAW264.7 cells
    Authors: YJ Kwon, EB Seo, SK Kim, KH Noh, H Lee, YW Joung, HM Shin, YA Jang, YM Kim, JT Lee, SK Ye
    Journal of ethnopharmacology, 2021;0(0):114493.  2021
  14. Microglia-derived interleukin-10 accelerates post-intracerebral hemorrhage hematoma clearance by regulating CD36
    Authors: Q Li, X Lan, X Han, F Durham, J Wan, A Weiland, RC Koehler, J Wang
    Brain, Behavior, and Immunity, 2021;94(0):437-457.  2021
  15. GL261 luciferase-expressing cells elicit an anti-tumor immune response: an evaluation of murine glioma models
    Authors: VE Sanchez, JP Lynes, S Walbridge, X Wang, NA Edwards, AK Nwankwo, HP Sur, GA Dominah, A Obungu, N Adamstein, PK Dagur, D Maric, J Munasinghe, JD Heiss, EK Nduom
    Sci Rep, 2020;10(1):11003.  2020
  16. Chronic Oral Palmitoylethanolamide Administration Rescues Cognitive Deficit and Reduces Neuroinflammation, Oxidative Stress, and Glutamate Levels in A Transgenic Murine Model of Alzheimer's Disease
    Authors: S Beggiato, MC Tomasini, T Cassano, L Ferraro
    J Clin Med, 2020;9(2):.  2020
  17. Cellular Virotherapy Increases Tumor-Infiltrating Lymphocytes (TIL) and Decreases their PD-1+ Subsets in Mouse Immunocompetent Models
    Authors: A Morales-Mo, MÁ Rodríguez-, A Gimenez-Sa, AJ Perisé-Bar, J García-Cas
    Cancers (Basel), 2020;12(7):.  2020
  18. TIAM2S Mediates Serotonin Homeostasis and Provokes a Pro-Inflammatory Immune Microenvironment Permissive for Colorectal Tumorigenesis
    Authors: YL Chan, WC Lai, JS Chen, JT Tseng, PC Chuang, J Jou, CT Lee, HS Sun
    Cancers (Basel), 2020;12(7):.  2020
  19. Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis
    Authors: M Asem, AM Young, C Oyama, AG Claure De, Y Liu, J Yang, TS Hilliard, J Johnson, EI Harper, I Guldner, S Zhang, TM Page-Maybe, WJ Kaliney, MS Stack
    Cancer Res., 2020;0(0):.  2020
  20. Tumor-infiltrating Leukocytes Suppress Local Inflammation Via Interleukin-1 Receptor Antagonist in a Syngeneic Prostate Cancer Model
    Authors: YC Fan, WY Chen, KD Lee, YC Tsai
    Biology (Basel), 2020;9(4):.  2020
  21. Midkine activation of CD8+ T cells establishes a neuron-immune-cancer axis responsible for low-grade glioma growth
    Authors: X Guo, Y Pan, M Xiong, S Sanapala, C Anastasaki, O Cobb, S Dahiya, DH Gutmann
    Nat Commun, 2020;11(1):2177.  2020
  22. Assessment of the Immunomodulatory Properties of the Probiotic Strain Lactobacillus paracasei K5 in vitro and In Vivo
    Authors: P Chondrou, A Karapetsas, DE Kiousi, S Vasileiadi, P Ypsilantis, S Botaitis, A Alexopoulo, S Plessas, E Bezirtzogl, A Galanis
    Microorganisms, 2020;8(5):.  2020
  23. Heterogeneity of response to immune checkpoint blockade in hypermutated experimental gliomas
    Authors: K Aslan, V Turco, J Blobner, JK Sonner, AR Liuzzi, NG Núñez, D De Feo, P Kickingere, M Fischer, E Green, A Sadik, M Friedrich, K Sanghvi, M Kilian, F Cichon, L Wolf, K Jähne, A von Landen, L Bunse, F Sahm, D Schrimpf, J Meyer, A Alexander, G Brugnara, R Röth, K Pfleiderer, B Niesler, A von Deimli, C Opitz, MO Breckwoldt, S Heiland, M Bendszus, W Wick, B Becher, M Platten
    Nat Commun, 2020;11(1):931.  2020
  24. Clonorchis sinensis MF6p/HDM (CsMF6p/HDM) induces pro-inflammatory immune response in RAW 264.7 macrophage cells via NF-&kappaB-dependent MAPK pathways
    Authors: JM Kang, WG Yoo, HG Lê, J Lee, WM Sohn, BK Na
    Parasit Vectors, 2020;13(1):20.  2020
  25. A novel STAT3 inhibitor attenuates angiotensin II-induced abdominal aortic aneurysm progression in mice through modulating vascular inflammation and autophagy
    Authors: QY Wu, Z Cheng, YZ Zhou, Y Zhao, JM Li, XM Zhou, HL Peng, GS Zhang, XB Liao, XM Fu
    Cell Death Dis, 2020;11(2):131.  2020
  26. Respiratory Epithelial Cells Respond to Lactobacillus plantarum but Provide No Cross-Protection against Virus-Induced Inflammation
    Authors: E Mai, CM Percopo, AR Limkar, AC Sek, M Ma, HF Rosenberg
    Viruses, 2020;13(1):.  2020
  27. IL-1&beta Induced Cytokine Expression by Spinal Astrocytes Can Play a Role in the Maintenance of Chronic Inflammatory Pain
    Authors: A Gajtkó, E Bakk, K Heged?s, L Ducza, K Holló
    Frontiers in Physiology, 2020;11(0):543331.  2020
  28. Loss of hepatocyte cell division leads to liver inflammation and fibrosis
    Authors: MR Dewhurst, JR Ow, G Zafer, NKM van Hul, H Wollmann, X Bisteau, D Brough, H Choi, P Kaldis
    PLoS Genet, 2020;16(11):e1009084.  2020
  29. Designed CXCR4 mimic acts as a soluble chemokine receptor that blocks atherogenic inflammation by agonist-specific targeting
    Authors: C Kontos, O El Bounkar, C Krammer, D Sinitski, K Hille, C Zan, G Yan, S Wang, Y Gao, M Brandhofer, RTA Megens, A Hoffmann, J Pauli, Y Asare, S Gerra, P Bourilhon, L Leng, HH Eckstein, WE Kempf, J Pelisek, O Gokce, L Maegdefess, R Bucala, M Dichgans, C Weber, A Kapurniotu, J Bernhagen
    Nat Commun, 2020;11(1):5981.  2020
  30. The nSMase2/Smpd3 gene modulates the severity of muscular dystrophy and the emotional stress response in mdx mice
    Authors: Y Matsuzaka, J Tanihata, Y Ooshima, D Yamada, M Sekiguchi, S Miyatake, Y Aoki, M Terumitsu, R Yashiro, H Komaki, A Ishiyama, Y Oya, YU Inoue, T Inoue, S Takeda, K Hashido
    BMC Med, 2020;18(1):343.  2020
  31. Calcitriol in the Presence of Conditioned Media from Metastatic Breast Cancer Cells Enhances Ex Vivo Polarization of M2 Alternative Murine Bone Marrow-Derived Macrophages
    Authors: A Anisiewicz, N ?ab?d?, I Krauze, J Wietrzyk
    Cancers (Basel), 2020;12(11):.  2020
  32. Extracellular vesicle-encapsulated IL-10 as novel nanotherapeutics against ischemic AKI
    Authors: TT Tang, B Wang, M Wu, ZL Li, Y Feng, JY Cao, D Yin, H Liu, RN Tang, SD Crowley, LL Lv, BC Liu
    Sci Adv, 2020;6(33):eaaz0748.  2020
  33. Xenogenization of tumor cells by fusogenic exosomes in tumor microenvironment ignites and propagates antitumor immunity
    Authors: GB Kim, GH Nam, Y Hong, J Woo, Y Cho, IC Kwon, Y Yang, IS Kim
    Science Advances, 2020;6(27):.  2020
  34. Onion Bulb Extract Downregulates EGFR/ERK1/2/AKT Signaling Pathway and Synergizes With Steroids to Inhibit Allergic Inflammation
    Authors: AZ El-Hashim, MA Khajah, KY Orabi, S Balakrishn, HG Sary, AA Abdelali
    Front Pharmacol, 2020;11(0):551683.  2020
  35. Inhibitory effect of ginsenoside Rg3 on cancer stemness and mesenchymal transition in breast cancer via regulation of myeloid-derived suppressor cells
    Authors: JH Song, DY Eum, SY Park, YH Jin, JW Shim, SJ Park, MY Kim, SJ Park, K Heo, YJ Choi
    PLoS ONE, 2020;15(10):e0240533.  2020
  36. Suppressing neutrophil-dependent angiogenesis abrogates resistance to anti-VEGF antibody in a genetic model of colorectal cancer
    Authors: Y Itatani, T Yamamoto, C Zhong, AA Molinolo, J Ruppel, P Hegde, MM Taketo, N Ferrara
    Proc. Natl. Acad. Sci. U.S.A., 2020;0(0):.  2020
  37. The voltage-gated proton channel Hv1 contributes to neuronal injury and motor deficits in a mouse model of spinal cord injury
    Authors: M Murugan, J Zheng, G Wu, R Mogilevsky, X Zheng, P Hu, J Wu, LJ Wu
    Mol Brain, 2020;13(1):143.  2020
  38. Loss of Par1b/MARK2 primes microglia during brain development and enhances their sensitivity to injury
    Authors: VL DiBona, W Zhu, MK Shah, A Rafalia, H Ben Cheikh, DP Crockett, H Zhang
    J Neuroinflammation, 2019;16(1):11.  2019
  39. Lysophosphatidic acid up-regulates IL-10 production to inhibit TNF-α synthesis in M?s stimulated with LPS
    Authors: A Ciesielska, A Hromada-Ju, E Ziemli?ska, K Kwiatkowsk
    J. Leukoc. Biol., 2019;0(0):.  2019
  40. Long-Lasting Anti-Inflammatory and Antinociceptive Effects of Acute Ammonium Glycyrrhizinate Administration: Pharmacological, Biochemical, and Docking Studies
    Authors: F Maione, P Minosi, A Di Giannua, F Raucci, MG Chini, S De Vita, G Bifulco, N Mascolo, S Pieretti
    Molecules, 2019;24(13):.  2019
  41. An in vitro model for studying CNS white matter: functional properties and experimental approaches
    Authors: S Bijland, G Thomson, M Euston, K Michail, K Thümmler, S Mücklisch, CL Crawford, SC Barnett, M McLaughlin, TJ Anderson, C Linington, ER Brown, ER Kalkman, JM Edgar
    F1000Res, 2019;8(0):117.  2019
  42. Yap/Taz regulate alveolar regeneration and resolution of lung inflammation
    Authors: R LaCanna, D Liccardo, P Zhang, L Tragesser, Y Wang, T Cao, HA Chapman, EE Morrisey, H Shen, WJ Koch, B Kosmider, MR Wolfson, Y Tian
    J. Clin. Invest., 2019;130(0):.  2019
  43. Functional Screening Identifies MicroRNAs as Multi-Cellular Regulators of Heart Failure
    Authors: R Verjans, WJA Derks, K Korn, B Sönnichsen, REW van Leeuwe, B Schroen, M van Bilsen, S Heymans
    Sci Rep, 2019;9(1):6055.  2019
  44. Low-Dose Tacrolimus Prevents Dysregulated Peri-Conceptional Ovarian and Systemic Immune Cellular Homeostasis in Subjects with PCOS
    Authors: AJH Albaghdadi, CA Feeley, FWK Kan
    Sci Rep, 2019;9(1):6528.  2019
  45. Plasma Rich in Growth Factors (PRGF) Disrupt the Blood-Brain Barrier Integrity and Elevate Amyloid Pathology in the Brains of 5XFAD Mice
    Authors: QV Duong, ML Kintzing, WE Kintzing, IM Abdallah, AD Brannen, A Kaddoumi
    Int J Mol Sci, 2019;20(6):.  2019
  46. Dose-Dependent Influences of Ethanol on Ischemic Stroke: Role of Inflammation
    Authors: G Xu, C Li, AL Parsiola, J Li, KD McCarter, R Shi, WG Mayhan, H Sun
    Front Cell Neurosci, 2019;13(0):6.  2019
  47. NK Cell-Mediated Processing Of Chlamydia psittaci Drives Potent Anti-Bacterial Th1 Immunity
    Authors: N Radomski, K Franzke, S Matthiesen, A Karger, MR Knittler
    Sci Rep, 2019;9(1):4799.  2019
  48. Suppression of chemotherapy-induced cytokine/lipid mediator surge and ovarian cancer by a dual COX-2/sEH inhibitor
    Authors: A Gartung, J Yang, VP Sukhatme, DR Bielenberg, D Fernandes, J Chang, BA Schmidt, SH Hwang, D Zurakowski, S Huang, MW Kieran, BD Hammock, D Panigrahy
    Proc. Natl. Acad. Sci. U.S.A., 2019;116(5):1698-1703.  2019
  49. Discrimination of Structural and Immunological Features of Polysaccharides from Persimmon Leaves at Different Maturity Stages
    Authors: YR Song, AR Han, TG Lim, JH Kang, HD Hong
    Molecules, 2019;24(2):.  2019
  50. Targeting myeloid-derived suppressor cells in combination with primary mammary tumor resection reduces metastatic growth in the lungs
    Authors: M Bosiljcic, RA Cederberg, MJ Hamilton, NE LePard, BT Harbourne, JL Collier, EC Halvorsen, R Shi, SE Franks, AY Kim, JP Banáth, M Hamer, FM Rossi, KL Bennewith
    Breast Cancer Res., 2019;21(1):103.  2019
  51. Iron accentuated reactive oxygen species release by NADPH oxidase in activated microglia contributes to oxidative stress in vitro
    Authors: YJ Yauger, S Bermudez, KE Moritz, E Glaser, B Stoica, KR Byrnes
    J Neuroinflammation, 2019;16(1):41.  2019
  52. Sirtuin 2 enhances allergic asthmatic inflammation
    Authors: YG Lee, BF Reader, D Herman, A Streicher, JA Englert, M Ziegler, S Chung, M Karpurapu, GY Park, JW Christman, MN Ballinger
    JCI Insight, 2019;0(0):.  2019
  53. Loss of the adhesion G-protein coupled receptor ADGRF5 in mice induces airway inflammation and the expression of CCL2 in lung endothelial cells
    Authors: F Kubo, DM Ariestanti, S Oki, T Fukuzawa, R Demizu, T Sato, RM Sabirin, S Hirose, N Nakamura
    Respir. Res., 2019;20(1):11.  2019
  54. The dynamic uptake and release of SOD3 from intracellular stores in macrophages modulates the inflammatory response
    Authors: L Hu, ED Zachariae, UG Larsen, F Vilhardt, SV Petersen
    Redox Biol, 2019;26(0):101268.  2019
  55. Fundamental differences in patterns of retinal ageing between primates and mice
    Authors: JH Kam, TW Weinrich, H Shinhmar, MB Powner, NW Roberts, A Aboelnour, G Jeffery
    Sci Rep, 2019;9(1):12574.  2019
  56. Acute Kahweol Treatment Attenuates Traumatic Brain Injury Neuroinflammation and Functional Deficits
    Authors: HF Lee, JS Lin, CF Chang
    Nutrients, 2019;11(10):.  2019
  57. Hearing impairment and associated morphological changes in pituitary adenylate cyclase activating polypeptide (PACAP)-deficient mice
    Authors: DB Fulop, V Humli, J Szepesy, V Ott, D Reglodi, B Gaszner, A Nemeth, A Szirmai, L Tamas, H Hashimoto, T Zelles, A Tamas
    Sci Rep, 2019;9(1):14598.  2019
  58. Zwitterionically modified alginates mitigate cellular overgrowth for cell encapsulation
    Authors: Q Liu, A Chiu, LH Wang, D An, M Zhong, AM Smink, BJ de Haan, P de Vos, K Keane, A Vegge, EY Chen, W Song, WF Liu, J Flanders, C Rescan, LG Grunnet, X Wang, M Ma
    Nat Commun, 2019;10(1):5262.  2019
  59. Paclitaxel-activated astrocytes produce mechanical allodynia in mice by releasing tumor necrosis factor-alpha and stromal-derived cell factor 1
    Authors: X Liu, R Tonello, Y Ling, YJ Gao, T Berta
    J Neuroinflammation, 2019;16(1):209.  2019
  60. Short-term inhibition of fibrinolytic system restores locomotor function after spinal cord injury in mice
    Authors: Y Shiraishi, A Kimura, O Matsuo, Y Sakata, K Takeshita, T Ohmori
    Sci Rep, 2019;9(1):16024.  2019
  61. Serum CXCL13 reflects local B-cell mediated inflammatory demyelinating peripheral neuropathy
    Authors: YH Kim, SY Jang, YK Shin, YR Jo, BA Yoon, SH Nam, BO Choi, HY Shin, SW Kim, SH Kim, JK Kim, HT Park
    Sci Rep, 2019;9(1):16535.  2019
  62. Mesothelial Cell HIF1alpha Expression Is Metabolically Downregulated by Metformin to Prevent Oncogenic Tumor-Stromal Crosstalk
    Authors: PC Hart, HA Kenny, N Grassl, KM Watters, LM Litchfield, F Coscia, I Blaženovi?, L Ploetzky, O Fiehn, M Mann, E Lengyel, IL Romero
    Cell Rep, 2019;29(12):4086-4098.e6.  2019
  63. Polarizing receptor activation dissociates fibroblast growth factor 2 mediated inhibition of myelination from its neuroprotective potential
    Authors: K Thümmler, E Rom, T Zeis, M Lindner, S Brunner, JJ Cole, D Arseni, S Mücklisch, JM Edgar, N Schaeren-W, A Yayon, C Linington
    Acta Neuropathol Commun, 2019;7(1):212.  2019
  64. A dual inhibitor of the proteasome catalytic subunits LMP2 and Y attenuates disease progression in mouse models of Alzheimer's disease
    Authors: IJ Yeo, MJ Lee, A Baek, Z Miller, D Bhattarai, YM Baek, HJ Jeong, YK Kim, DE Kim, JT Hong, KB Kim
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    J. Exp. Med., 2010;207(11):2343-54.  2010
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    Authors: Diao J, Zhao J, Winter E, Cattral MS
    J. Immunol., 2010;184(3):1261-7.  2010
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FAQs

  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

View all Proteome Profiler Antibody Array FAQs

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Reviews for Proteome Profiler Mouse Cytokine Array Kit, Panel A

Average Rating: 4.8 (Based on 31 Reviews)

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Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 10/01/2021
Application:

The array was used to test the anti-inflammatory potential from Octopus vulgaris ink extracts on LPS-stimulated RAW 264.7 cells. Results obtained were in agreement with other biochemical and in vitro test. We have used this kit in several LPS-challenged murine macrophages. Fig. A shows the expression level of each cytokine against the control (LPS only-treated macrophages); B shows a bioinformatic arrangement of the modulated cytokines, and C shows a predictive analysis from the results obtained in this kit.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 09/23/2021
Application:

Pro and anti-inflammatory cytokines were evaluated in RAW264.7 macrophages treated with LPS (1 microgram/mL) and a South American berry (ABJ). Results obtained showed the modulation of several cytokines indicated in this kit. We have conducted several evaluations of these cytokines using this kit and results never disappoint. What we like the most is that this assay allows the evaluation of several cytokines that would take a lot of time by conventional techniques such as ELISA or Western Blot.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 09/23/2021
Application:

20 ul serum was used to perform the experiment.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 08/31/2021
Application:
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 08/25/2021
Application:

mouse serum 200ul used for cancer model

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 08/17/2021
Application:

Used serum to see the role of gene in pavreatic cancer

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 05/10/2021
Application:

Phenomenal results, consistent performance and excellent translation to use with Streptavidin-800. On step 11, I swapped the Streptavidin-HRP for a 1:2,000 ratio of Streptavidin-800. Then followed with the wash steps and the result is as posted.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 03/06/2021
Application:
Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/24/2020
Application:

150UL SERUM WAS USED

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/12/2020
Application:

mouse tissues

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/12/2020
Application:

serum from knock-out mice

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/08/2020
Application:
Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 12/10/2019
Application:

Followed protocol exactly, 10 min exposure on film wasn't great, boosted to 20 min exposure, still not good. 5 min on digital imager and positive control spots were saturated! Much more sensitive!

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 06/06/2019
Application:

T cells isolated and stimulated with various agonists.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 04/05/2019
Application:

good!

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 03/13/2019
Application:

This array was used to measure the modulation of inflammation-associated cytokines in LPS-stimulated RAW264.7 macrophages treated with several doses of the non-digestible fraction from a corn/bean snack.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/07/2019
Application:
Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 12/26/2018
Application:
Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 12/11/2018
Application:
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 11/11/2018
Application:

High quality results with simple and straightforward protocol. No optimization needed.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 07/19/2018
Application:

instructions easy to follow. I needed a little longer than the 10 minute exposure due to low protein concentrations from my very small tissues.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 04/26/2018
Application:
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 10/20/2017
Application:
Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 09/27/2017
Application:

Very sensitive cytokine assay!

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 07/14/2017
Application:

performed on cell conditoned media from mouse fibroblasts

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 05/10/2017
Application:
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 03/15/2017
Application:

Assay tested in 200 ug of protein from pool of intestines of 10 dpf zebrafish larvae.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/27/2017
Application:

Mouse BALF samples from pooled control and treatment groups in 2 separate arrays to determine the altered cytokine response.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 01/27/2017
Application:

Bronchoalveolar lavage fluid from C57BL/6J mouse

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 10/06/2016
Application:
Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 09/29/2016
Application:
Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006