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Proteome Profiler Mouse Cytokine Array Kit, Panel A

ARY006 235 Citations 23 Reviews Datasheet / COA / SDS
Contains 4 membranes - each spotted in duplicate with 40 different cytokine antibodies
Catalog # Availability Size / Price Qty
ARY006
The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample.
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Citations (235)
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Proteome Profiler Mouse Cytokine Array Kit, Panel A Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.

Troubleshooting Guide

Key Benefits

  • Detects 40 mouse cytokines, chemokines, and acute phase proteins simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR and chemiluminescence detection

Principle of the Assay

The Proteome Profiler Mouse Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Mouse Cytokine Array, Panel A nitrocellulose membranes spotted with 40 different antibodies to mouse cytokines, chemokines, and acute phase proteins
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Mouse Cytokine Array, Panel A
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.  

 
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
CXCL13/BLC/BCA-1 IL-5 M-CSF
C5a IL-6 CCL2/JE/MCP-1
G-CSF IL-7 CCL12/MCP-5
GM-CSF IL-10 CXCL9/MIG
CCL1/I-309 IL-12 p70 CCL3/MIP-1 alpha
CCL11/Eotaxin IL-13 CCL4/MIP-1 beta
ICAM-1 IL-16 CXCL2/MIP-2
IFN-gamma IL-17 CCL5/RANTES
IL-1 alpha/IL-1F1 IL-23 CXCL12/SDF-1
IL-1 beta/IL-1F2 IL-27 CCL17/TARC
IL-1ra/IL-1F3 CXCL10/IP-10 TIMP-1
IL-2 CXCL11/I-TAC TNF-alpha
IL-3 CXCL1/KC TREM-1
IL-4    

 

Assays for Analytes represented in the Mouse Cytokine Array Kit, Panel A

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
CXCL13/BLC/BCA-1 MCX130 DY470
C5a   DY2150
G-CSF MCS00 DY414
GM-CSF MGM00 DY415
CCL1/I-309/TCA-3   DY845
CCL11/Eotaxin MME00 DY420
ICAM-1 MIC100 DY796
IFN-gamma MIF00 DY485
IL-1 alpha MLA00 DY400
IL-1 beta MLB00B DY401
IL-1ra MRA00 DY480
IL-2 M2000 DY402
IL-3 M3000 DY403
IL-4 M4000B DY404
IL-5 M5000 DY405
IL-6 M6000B DY406
IL-7 M7000 DY407
IL-10 M1000b DY417
IL-12 p70 M1270 DY419
IL-13 M1300CB DY413
IL-16    
IL-17 M1700 DY421
IL-23 M2300 DY1887
IL-27 M2728  
CXCL10/IP-10/CRG-2 MCX100 DY466
CXCL11/I-TAC   DY572
CXCL1/KC MKC00B DY453
M-CSF MMC00 DY416
CCL2/MCP-1/JE MJE00 DY479
CCL12/MCP-5 MCC120 DY428
CXCL9/MIG MCX900 DY492
CCL3/MIP-1 alpha MMA00 DY450
CCL4/MIP-1 beta MMB00 DY451
CXCL2/MIP-2 MM200 DY452
CCL5/RANTES MMR00 DY478
CXCL12/SDF-1 MCX120 DY460
CCL17/TARC   DY529
TIMP-1 MTM100 DY980
TNF-alpha MTA00 DY410
TREM-1 MTRM10 DY1187

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Mouse

Product Datasheets

Product Datasheet
COA
SDS

Data Example

The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. View Larger

The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. Mouse splenocytes treated with 100 ng/mL LPS for 20 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Mouse Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane.

The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. View Larger

The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. (A. and B.) Lysates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µg of lysate was used for each array shown. (C. and D.) Supernates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates. View Larger

The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates. (A and B.) Samples of mouse serum from two different vendors. 200 µL of serum was used for each array shown. (C. and D.) Lysates of mouse lung tissue and mouse stomach tissue. 200 µg of lysate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

Citations for Proteome Profiler Mouse Cytokine Array Kit, Panel A

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

235 Citations: Showing 1 - 10
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  1. Cellular Virotherapy Increases Tumor-Infiltrating Lymphocytes (TIL) and Decreases their PD-1+ Subsets in Mouse Immunocompetent Models
    Authors: A Morales-Mo, MÁ Rodríguez-, A Gimenez-Sa, AJ Perisé-Bar, J García-Cas
    Cancers (Basel), 2020;12(7):.  2020
  2. Tumor-infiltrating Leukocytes Suppress Local Inflammation Via Interleukin-1 Receptor Antagonist in a Syngeneic Prostate Cancer Model
    Authors: YC Fan, WY Chen, KD Lee, YC Tsai
    Biology (Basel), 2020;9(4):.  2020
  3. Host Wnt5a Potentiates Microenvironmental Regulation of Ovarian Cancer Metastasis
    Authors: M Asem, AM Young, C Oyama, AG Claure De, Y Liu, J Yang, TS Hilliard, J Johnson, EI Harper, I Guldner, S Zhang, TM Page-Maybe, WJ Kaliney, MS Stack
    Cancer Res., 2020;0(0):.  2020
  4. Assessment of the Immunomodulatory Properties of the Probiotic Strain Lactobacillus paracasei K5 in vitro and In Vivo
    Authors: P Chondrou, A Karapetsas, DE Kiousi, S Vasileiadi, P Ypsilantis, S Botaitis, A Alexopoulo, S Plessas, E Bezirtzogl, A Galanis
    Microorganisms, 2020;8(5):.  2020
  5. Midkine activation of CD8+ T cells establishes a neuron-immune-cancer axis responsible for low-grade glioma growth
    Authors: X Guo, Y Pan, M Xiong, S Sanapala, C Anastasaki, O Cobb, S Dahiya, DH Gutmann
    Nat Commun, 2020;11(1):2177.  2020
  6. Clonorchis sinensis MF6p/HDM (CsMF6p/HDM) induces pro-inflammatory immune response in RAW 264.7 macrophage cells via NF-&kappaB-dependent MAPK pathways
    Authors: JM Kang, WG Yoo, HG Lê, J Lee, WM Sohn, BK Na
    Parasit Vectors, 2020;13(1):20.  2020
  7. GL261 luciferase-expressing cells elicit an anti-tumor immune response: an evaluation of murine glioma models
    Authors: VE Sanchez, JP Lynes, S Walbridge, X Wang, NA Edwards, AK Nwankwo, HP Sur, GA Dominah, A Obungu, N Adamstein, PK Dagur, D Maric, J Munasinghe, JD Heiss, EK Nduom
    Sci Rep, 2020;10(1):11003.  2020
  8. TIAM2S Mediates Serotonin Homeostasis and Provokes a Pro-Inflammatory Immune Microenvironment Permissive for Colorectal Tumorigenesis
    Authors: YL Chan, WC Lai, JS Chen, JT Tseng, PC Chuang, J Jou, CT Lee, HS Sun
    Cancers (Basel), 2020;12(7):.  2020
  9. Fundamental differences in patterns of retinal ageing between primates and mice
    Authors: JH Kam, TW Weinrich, H Shinhmar, MB Powner, NW Roberts, A Aboelnour, G Jeffery
    Sci Rep, 2019;9(1):12574.  2019
  10. Yap/Taz regulate alveolar regeneration and resolution of lung inflammation
    Authors: R LaCanna, D Liccardo, P Zhang, L Tragesser, Y Wang, T Cao, HA Chapman, EE Morrisey, H Shen, WJ Koch, B Kosmider, MR Wolfson, Y Tian
    J. Clin. Invest., 2019;130(0):.  2019
  11. Length and diameter-dependent phagocytosis and cytotoxicity of long silver nanowires in macrophages
    Authors: F Wang, Y Wang, X Yao, C Ma, Y Yin, M Song
    Chemosphere, 2019;237(0):124565.  2019
  12. Targeting myeloid-derived suppressor cells in combination with primary mammary tumor resection reduces metastatic growth in the lungs
    Authors: M Bosiljcic, RA Cederberg, MJ Hamilton, NE LePard, BT Harbourne, JL Collier, EC Halvorsen, R Shi, SE Franks, AY Kim, JP Banáth, M Hamer, FM Rossi, KL Bennewith
    Breast Cancer Res., 2019;21(1):103.  2019
  13. The dynamic uptake and release of SOD3 from intracellular stores in macrophages modulates the inflammatory response
    Authors: L Hu, ED Zachariae, UG Larsen, F Vilhardt, SV Petersen
    Redox Biol, 2019;26(0):101268.  2019
  14. Lysophosphatidic acid up-regulates IL-10 production to inhibit TNF-α synthesis in M?s stimulated with LPS
    Authors: A Ciesielska, A Hromada-Ju, E Ziemli?ska, K Kwiatkowsk
    J. Leukoc. Biol., 2019;0(0):.  2019
  15. Long-Lasting Anti-Inflammatory and Antinociceptive Effects of Acute Ammonium Glycyrrhizinate Administration: Pharmacological, Biochemical, and Docking Studies
    Authors: F Maione, P Minosi, A Di Giannua, F Raucci, MG Chini, S De Vita, G Bifulco, N Mascolo, S Pieretti
    Molecules, 2019;24(13):.  2019
  16. An in vitro model for studying CNS white matter: functional properties and experimental approaches
    Authors: S Bijland, G Thomson, M Euston, K Michail, K Thümmler, S Mücklisch, CL Crawford, SC Barnett, M McLaughlin, TJ Anderson, C Linington, ER Brown, ER Kalkman, JM Edgar
    F1000Res, 2019;8(0):117.  2019
  17. Acute Kahweol Treatment Attenuates Traumatic Brain Injury Neuroinflammation and Functional Deficits
    Authors: HF Lee, JS Lin, CF Chang
    Nutrients, 2019;11(10):.  2019
  18. Dose-Dependent Influences of Ethanol on Ischemic Stroke: Role of Inflammation
    Authors: G Xu, C Li, AL Parsiola, J Li, KD McCarter, R Shi, WG Mayhan, H Sun
    Front Cell Neurosci, 2019;13(0):6.  2019
  19. Functional Screening Identifies MicroRNAs as Multi-Cellular Regulators of Heart Failure
    Authors: R Verjans, WJA Derks, K Korn, B Sönnichsen, REW van Leeuwe, B Schroen, M van Bilsen, S Heymans
    Sci Rep, 2019;9(1):6055.  2019
  20. Low-Dose Tacrolimus Prevents Dysregulated Peri-Conceptional Ovarian and Systemic Immune Cellular Homeostasis in Subjects with PCOS
    Authors: AJH Albaghdadi, CA Feeley, FWK Kan
    Sci Rep, 2019;9(1):6528.  2019
  21. Mesenchymal stem cell-derived conditioned medium attenuate angiotensin II-induced aortic aneurysm growth by modulating macrophage polarization
    Authors: YZ Zhou, Z Cheng, Y Wu, QY Wu, XB Liao, Y Zhao, JM Li, XM Zhou, XM Fu
    J. Cell. Mol. Med., 2019;0(0):.  2019
  22. NK Cell-Mediated Processing Of Chlamydia psittaci Drives Potent Anti-Bacterial Th1 Immunity
    Authors: N Radomski, K Franzke, S Matthiesen, A Karger, MR Knittler
    Sci Rep, 2019;9(1):4799.  2019
  23. Plasma Rich in Growth Factors (PRGF) Disrupt the Blood-Brain Barrier Integrity and Elevate Amyloid Pathology in the Brains of 5XFAD Mice
    Authors: QV Duong, ML Kintzing, WE Kintzing, IM Abdallah, AD Brannen, A Kaddoumi
    Int J Mol Sci, 2019;20(6):.  2019
  24. Suppression of chemotherapy-induced cytokine/lipid mediator surge and ovarian cancer by a dual COX-2/sEH inhibitor
    Authors: A Gartung, J Yang, VP Sukhatme, DR Bielenberg, D Fernandes, J Chang, BA Schmidt, SH Hwang, D Zurakowski, S Huang, MW Kieran, BD Hammock, D Panigrahy
    Proc. Natl. Acad. Sci. U.S.A., 2019;116(5):1698-1703.  2019
  25. Iron accentuated reactive oxygen species release by NADPH oxidase in activated microglia contributes to oxidative stress in vitro
    Authors: YJ Yauger, S Bermudez, KE Moritz, E Glaser, B Stoica, KR Byrnes
    J Neuroinflammation, 2019;16(1):41.  2019
  26. Sirtuin 2 enhances allergic asthmatic inflammation
    Authors: YG Lee, BF Reader, D Herman, A Streicher, JA Englert, M Ziegler, S Chung, M Karpurapu, GY Park, JW Christman, MN Ballinger
    JCI Insight, 2019;0(0):.  2019
  27. Loss of the adhesion G-protein coupled receptor ADGRF5 in mice induces airway inflammation and the expression of CCL2 in lung endothelial cells
    Authors: F Kubo, DM Ariestanti, S Oki, T Fukuzawa, R Demizu, T Sato, RM Sabirin, S Hirose, N Nakamura
    Respir. Res., 2019;20(1):11.  2019
  28. Discrimination of Structural and Immunological Features of Polysaccharides from Persimmon Leaves at Different Maturity Stages
    Authors: YR Song, AR Han, TG Lim, JH Kang, HD Hong
    Molecules, 2019;24(2):.  2019
  29. Pseudomonas aeruginosa Lipoxygenase LoxA Contributes to Lung Infection by Altering the Host Immune Lipid Signaling
    Authors: E Morello, T Pérez-Bere, C Boisseau, T Baranek, A Guillon, D Bréa, P Lanotte, X Carpena, N Pietrancos, V Hervé, R Ramphal, N Cenac, M Si-Tahar
    Front Microbiol, 2019;10(0):1826.  2019
  30. Loss of Par1b/MARK2 primes microglia during brain development and enhances their sensitivity to injury
    Authors: VL DiBona, W Zhu, MK Shah, A Rafalia, H Ben Cheikh, DP Crockett, H Zhang
    J Neuroinflammation, 2019;16(1):11.  2019
  31. Hearing impairment and associated morphological changes in pituitary adenylate cyclase activating polypeptide (PACAP)-deficient mice
    Authors: DB Fulop, V Humli, J Szepesy, V Ott, D Reglodi, B Gaszner, A Nemeth, A Szirmai, L Tamas, H Hashimoto, T Zelles, A Tamas
    Sci Rep, 2019;9(1):14598.  2019
  32. Mesothelial Cell HIF1alpha Expression Is Metabolically Downregulated by Metformin to Prevent Oncogenic Tumor-Stromal Crosstalk
    Authors: PC Hart, HA Kenny, N Grassl, KM Watters, LM Litchfield, F Coscia, I Blaženovi?, L Ploetzky, O Fiehn, M Mann, E Lengyel, IL Romero
    Cell Rep, 2019;29(12):4086-4098.e6.  2019
  33. Short-term inhibition of fibrinolytic system restores locomotor function after spinal cord injury in mice
    Authors: Y Shiraishi, A Kimura, O Matsuo, Y Sakata, K Takeshita, T Ohmori
    Sci Rep, 2019;9(1):16024.  2019
  34. Zwitterionically modified alginates mitigate cellular overgrowth for cell encapsulation
    Authors: Q Liu, A Chiu, LH Wang, D An, M Zhong, AM Smink, BJ de Haan, P de Vos, K Keane, A Vegge, EY Chen, W Song, WF Liu, J Flanders, C Rescan, LG Grunnet, X Wang, M Ma
    Nat Commun, 2019;10(1):5262.  2019
  35. Targeting tumor-resident mast cells for effective anti-melanoma immune responses
    Authors: S Kaesler, F Wölbing, WE Kempf, Y Skabytska, M Köberle, T Volz, T Sinnberg, T Amaral, S Möckel, A Yazdi, G Metzler, M Schaller, K Hartmann, B Weide, C Garbe, HG Rammensee, M Röcken, T Biedermann
    JCI Insight, 2019;4(19):.  2019
  36. Serum CXCL13 reflects local B-cell mediated inflammatory demyelinating peripheral neuropathy
    Authors: YH Kim, SY Jang, YK Shin, YR Jo, BA Yoon, SH Nam, BO Choi, HY Shin, SW Kim, SH Kim, JK Kim, HT Park
    Sci Rep, 2019;9(1):16535.  2019
  37. Paclitaxel-activated astrocytes produce mechanical allodynia in mice by releasing tumor necrosis factor-alpha and stromal-derived cell factor 1
    Authors: X Liu, R Tonello, Y Ling, YJ Gao, T Berta
    J Neuroinflammation, 2019;16(1):209.  2019
  38. Polarizing receptor activation dissociates fibroblast growth factor 2 mediated inhibition of myelination from its neuroprotective potential
    Authors: K Thümmler, E Rom, T Zeis, M Lindner, S Brunner, JJ Cole, D Arseni, S Mücklisch, JM Edgar, N Schaeren-W, A Yayon, C Linington
    Acta Neuropathol Commun, 2019;7(1):212.  2019
  39. Deletion of the Mir-106b~?25 MicroRNA cluster attenuates atherosclerosis in Apolipoprotein E knockout mice
    Authors: J Semo, G Chernin, M Jonas, S Shimoni, J George
    Lipids Health Dis, 2019;18(1):208.  2019
  40. A dual inhibitor of the proteasome catalytic subunits LMP2 and Y attenuates disease progression in mouse models of Alzheimer's disease
    Authors: IJ Yeo, MJ Lee, A Baek, Z Miller, D Bhattarai, YM Baek, HJ Jeong, YK Kim, DE Kim, JT Hong, KB Kim
    Sci Rep, 2019;9(1):18393.  2019
  41. Compensatory regrowth of the mouse bladder after partial cystectomy
    Authors: GB Delos Sant, MY Devine, J Wetterlin, PR Firmiss, NA Kukulka, DK Bowen, EM Gong, RW Dettman
    PLoS ONE, 2018;13(11):e0206436.  2018
  42. Sarcodon imbricatus polysaccharides improve mouse hematopoietic function after cyclophosphamide-induced damage via G-CSF mediated JAK2/STAT3 pathway
    Authors: X Wang, Q Chu, X Jiang, Y Yu, L Wang, Y Cui, J Lu, L Teng, D Wang
    Cell Death Dis, 2018;9(6):578.  2018
  43. HEPES activates a MiT/TFE-dependent lysosomal-autophagic gene network in cultured cells: A call for caution
    Authors: MJ Tol, MJC van der Li, TL Gabriel, JJ Hagen, S Scheij, T Veenendaal, J Klumperman, WE Donker-Koo, AJ Verhoeven, H Overkleeft, JM Aerts, CA Argmann, M van Eijk
    Autophagy, 2018;0(0):1-13.  2018
  44. The role of HFE genotype in macrophage phenotype
    Authors: AM Nixon, E Neely, IA Simpson, JR Connor
    J Neuroinflammation, 2018;15(1):30.  2018
  45. Impaired immune surveillance accelerates accumulation of senescent cells and aging
    Authors: Y Ovadya, T Landsberge, H Leins, E Vadai, H Gal, A Biran, R Yosef, A Sagiv, A Agrawal, A Shapira, J Windheim, M Tsoory, R Schirmbeck, I Amit, H Geiger, V Krizhanovs
    Nat Commun, 2018;9(1):5435.  2018
  46. Myeloid p38? signaling promotes intestinal IGF-1 production and inflammation-associated tumorigenesis
    Authors: C Youssif, M Cubillos-R, M Comalada, E Llonch, C Perna, N Djouder, AR Nebreda
    EMBO Mol Med, 2018;0(0):.  2018
  47. A non-cell-autonomous role for Pml in the maintenance of leukemia from the niche
    Authors: J Guarnerio, LM Mendez, N Asada, AV Menon, J Fung, K Berry, PS Frenette, K Ito, PP Pandolfi
    Nat Commun, 2018;9(1):66.  2018
  48. Monocyte infiltration rather than microglia proliferation dominates the early immune response to rapid photoreceptor degeneration
    Authors: SJ Karlen, EB Miller, X Wang, ES Levine, RJ Zawadzki, ME Burns
    J Neuroinflammation, 2018;15(1):344.  2018
  49. Rab4b Deficiency in T Cells Promotes Adipose Treg/Th17 Imbalance, Adipose Tissue Dysfunction, and Insulin Resistance
    Authors: J Gilleron, G Bouget, S Ivanov, C Meziat, F Ceppo, B Vergoni, M Djedaini, A Soprani, K Dumas, A Jacquel, L Yvan-Charv, N Venteclef, JF Tanti, M Cormont
    Cell Rep, 2018;25(12):3329-3341.e5.  2018
  50. Therapeutic effects of telomerase in mice with pulmonary fibrosis induced by damage to the lungs and short telomeres
    Authors: JM Povedano, P Martinez, R Serrano, Á Tejera, G Gómez-Lópe, M Bobadilla, JM Flores, F Bosch, MA Blasco
    Elife, 2018;7(0):.  2018
  51. Unique pattern of neutrophil migration and function during tumor progression
    Authors: S Patel, S Fu, J Mastio, GA Dominguez, A Purohit, A Kossenkov, C Lin, K Alicea-Tor, M Sehgal, Y Nefedova, J Zhou, LR Languino, C Clendenin, RH Vonderheid, C Mulligan, B Nam, N Hockstein, G Masters, M Guarino, ZT Schug, DC Altieri, DI Gabrilovic
    Nat. Immunol., 2018;19(11):1236-1247.  2018
  52. LINCS L1000 dataset-based repositioning of CGP-60474 as a highly potent anti-endotoxemic agent
    Authors: HW Han, S Hahn, HY Jeong, JH Jee, MO Nam, HK Kim, DH Lee, SY Lee, DK Choi, JH Yu, SH Min, J Yoo
    Sci Rep, 2018;8(1):14969.  2018
  53. Menthol, a unique urinary volatile compound, is associated with chronic inflammation in interstitial cystitis
    Authors: M Shahid, MY Lee, A Yeon, E Cho, V Sairam, L Valdiviez, S You, J Kim
    Sci Rep, 2018;8(1):10859.  2018
  54. A severe atherosclerosis mouse model on the resistant NOD background
    Authors: X Wang, R Huang, L Zhang, S Li, J Luo, Y Gu, Z Chen, Q Zheng, T Chao, W Zheng, X Qi, L Wang, Y Wen, Y Liang, L Lu
    Dis Model Mech, 2018;11(10):.  2018
  55. Tobacco Carcinogen-Induced Production of GM-CSF Activates CREB to Promote Pancreatic Cancer
    Authors: S Srinivasan, T Totiger, C Shi, J Castellano, P Lamichhane, AR Dosch, F Messaggio, N Kashikar, K Honnenahal, Y Ban, NB Merchant, MN VanSaun, NS Nagathihal
    Cancer Res., 2018;0(0):.  2018
  56. Administration of cytokine-induced myeloid-derived suppressor cells ameliorates renal fibrosis in diabetic mice
    Authors: CC Hsieh, CL Lin, JT He, M Chiang, Y Wang, YC Tsai, CH Hung, PJ Chang
    Stem Cell Res Ther, 2018;9(1):183.  2018
  57. Inflammaging impairs peripheral nerve maintenance and regeneration
    Authors: R Büttner, A Schulz, M Reuter, AK Akula, T Mindos, A Carlstedt, LB Riecken, SL Baader, R Bauer, H Morrison
    Aging Cell, 2018;0(0):e12833.  2018
  58. Caveolin-1-Knockout Mouse as a Model of Inflammatory Diseases
    Authors: E Codrici, L Albulescu, ID Popescu, S Mihai, AM Enciu, R Albulescu, C Tanase, ME Hinescu
    J Immunol Res, 2018;2018(0):2498576.  2018
  59. Bone marrow mesenchymal stem cells tune the differentiation of myeloid-derived suppressor cells in bleomycin-induced lung injury
    Authors: X Su, L Yang, Y Yin, J Huang, F Qiao, Y Fang, L Yu, Y Wang, K Zhou, J Wang
    Stem Cell Res Ther, 2018;9(1):253.  2018
  60. CLEC16A regulates splenocyte and NK cell function in part through MEK signaling
    Authors: R Pandey, M Bakay, HS Hain, B Strenkowsk, BZB Elsaqa, JD Roizen, JA Kushner, JS Orange, H Hakonarson
    PLoS ONE, 2018;13(9):e0203952.  2018
  61. Akt1-Mediated Muscle Growth Promotes Blood Flow Recovery After Hindlimb Ischemia by Enhancing Heme Oxygenase-1 in Neighboring Cells
    Authors: Y Onoue, Y Izumiya, S Hanatani, T Ishida, Y Arima, S Yamamura, Y Kimura, S Araki, M Ishii, T Nakamura, Y Oimatsu, K Sakamoto, E Yamamoto, S Kojima, K Kaikita, K Tsujita
    Circ. J., 2018;0(0):.  2018
  62. Molecular and cellular identification of the immune response in peripheral ganglia following nerve injury
    Authors: JA Lindborg, JP Niemi, MA Howarth, KW Liu, CZ Moore, D Mahajan, RE Zigmond
    J Neuroinflammation, 2018;15(1):192.  2018
  63. Choline transport links macrophage phospholipid metabolism and inflammation
    Authors: SA Snider, KD Margison, P Ghorbani, ND LeBlond, C O'Dwyer, JR Nunes, T Nguyen, H Xu, SA Bennett, MD Fullerton
    J. Biol. Chem., 2018;0(0):.  2018
  64. Impact of eosinophil-peroxidase (EPX) deficiency on eosinophil structure and function in mouse airways
    Authors: CM Percopo, JO Krumholz, ER Fischer, LS Kraemer, M Ma, K Laky, HF Rosenberg
    J. Leukoc. Biol., 2018;0(0):.  2018
  65. Human Adipose Tissue-Derived Mesenchymal Stem Cells Attenuate Atopic Dermatitis by Regulating the Expression of MIP-2, miR-122a-SOCS1 Axis, and Th1/Th2 Responses
    Authors: M Kim, SH Lee, Y Kim, Y Kwon, Y Park, HK Lee, HS Jung, D Jeoung
    Front Pharmacol, 2018;9(0):1175.  2018
  66. Gene and Protein Expression Profiles in a Mouse Model of Collagen-Induced Arthritis
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    Sci Rep, 2014;4(0):6383.  2014
  165. Novel mechanisms underlying the immediate and transient global tolerization of splenic dendritic cells after vaccination with a self-antigen.
    Authors: Farkas A, Finn O
    J Immunol, 2014;192(2):658-65.  2014
  166. IL-15 deficient tax mice reveal a role for IL-1alpha in tumor immunity.
    Authors: Rauch D, Harding J, Ratner L
    PLoS ONE, 2014;9(1):e85028.  2014
  167. BMP pathway regulation of and by macrophages.
    Authors: Talati M, West J, Zaynagetdinov R, Hong C, Han W, Blackwell T, Robinson L, Blackwell T, Lane K
    PLoS ONE, 2014;9(4):e94119.  2014
  168. The pathogenic mechanism of the Mycobacterium ulcerans virulence factor, mycolactone, depends on blockade of protein translocation into the ER.
    Authors: Hall, Belinda, Hill, Kirsti, McKenna, Michael, Ogbechi, Joy, High, Stephen, Willis, Anne E, Simmonds, Rachel E
    PLoS Pathog, 2014;10(4):e1004061.  2014
  169. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) prevents lipopolysaccharide (LPS)-induced, sepsis-related severe acute lung injury in mice.
    Authors: Takaoka, Yuki, Goto, Shigeru, Nakano, Toshiaki, Tseng, Hui-Peng, Yang, Shih-Min, Kawamoto, Seiji, Ono, Kazuhisa, Chen, Chao-Lon
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  171. Metabolic reprogramming of macrophages: glucose transporter 1 (GLUT1)-mediated glucose metabolism drives a proinflammatory phenotype.
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  172. Dystrophic mdx mice develop severe cardiac and respiratory dysfunction following genetic ablation of the anti-inflammatory cytokine IL-10.
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  174. Role of Rip2 in development of tumor-infiltrating MDSCs and bladder cancer metastasis.
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    PLoS ONE, 2014;9(4):e94793.  2014
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    Mucosal Immunol, 2012;5(0):702.  2012
  186. Dietary blue pigments derived from genipin, attenuate inflammation by inhibiting LPS-induced iNOS and COX-2 expression via the NF-kappaB inactivation.
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    PLoS ONE, 2012;7(3):e34122.  2012
  187. Early-outgrowth bone marrow cells attenuate renal injury and dysfunction via an antioxidant effect in a mouse model of type 2 diabetes.
    Diabetes, 2012;61(8):2114-25.  2012
  188. Histone Deacetylase 3 Mediates Allergic Skin Inflammation by Regulating Expression of MCP1 Protein.
    Authors: Kim Y, Kim K, Park D, Lee E, Lee H, Lee YS, Choe J, Jeoung D
    J. Biol. Chem., 2012;287(31):25844-59.  2012
  189. The synthetic triterpenoid CDDO-methyl ester delays estrogen receptor-negative mammary carcinogenesis in polyoma middle T mice.
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    Cancer Prev Res (Phila), 2012;5(5):726-34.  2012
  190. Exosomes isolated from mycobacteria-infected mice or cultured macrophages can recruit and activate immune cells in vitro and in vivo.
    Authors: Singh PP, Smith VL, Karakousis PC, Schorey JS
    J. Immunol., 2012;189(2):777-85.  2012
  191. A new human DSG2-transgenic mouse model for studying the tropism and pathology of human adenoviruses.
    Authors: Wang H, Beyer I, Persson J, Song H, Li Z, Richter M, Cao H, van Rensburg R, Yao X, Hudkins K, Yumul R, Zhang XB, Yu M, Fender P, Hemminki A, Lieber A
    J. Virol., 2012;86(11):6286-302.  2012
  192. The role of alveolar epithelial cells in initiating and shaping pulmonary immune responses: communication between innate and adaptive immune systems.
    Authors: Chuquimia OD, Petursdottir DH, Rahman MJ, Hartl K, Singh M, Fernandez C
    PLoS ONE, 2012;7(2):e32125.  2012
  193. Clusterin induces the secretion of TNF-α and the chemotactic migration of macrophages.
    Authors: Shim YJ, Kang BH, Choi BK, Park IS, Min BH
    Biochem. Biophys. Res. Commun., 2012;422(1):200-5.  2012
  194. The MSHA Strain of Pseudomonas Aeruginosa Activated TLR Pathway and Enhanced HIV-1 DNA Vaccine Immunoreactivity.
    Authors: Hou J, Liu Y, Liu Y, Shao Y
    PLoS ONE, 2012;7(10):e47724.  2012
  195. Liver-specific expression of transcriptionally active SREBP-1c is associated with fatty liver and increased visceral fat mass.
    Authors: Knebel B, Haas J, Hartwig S, Jacob S, Kollmer C, Nitzgen U, Muller-Wieland D, Kotzka J
    PLoS ONE, 2012;7(2):e31812.  2012
  196. Macrophage Dectin-1 Expression Is Controlled by Leukotriene B4 via a GM-CSF/PU.1 Axis.
    Authors: Serezani CH, Kane S, Collins L
    J. Immunol., 2012;189(2):906-15.  2012
  197. A novel small molecule, HK-156, inhibits lipopolysaccharide-induced activation of NF-kappaB signaling and improves survival in mouse models of sepsis.
    Acta Pharmacol. Sin., 2012;33(9):1204-16.  2012
  198. Chitin elicits CCL2 from airway epithelial cells and induces CCR2-dependent innate allergic inflammation in the lung.
    J. Immunol., 2012;189(5):2545-52.  2012
  199. NLRP3 suppresses NK cell-mediated responses to carcinogen-induced tumors and metastases.
    Authors: Chow M, Sceneay J, Paget C, Wong C, Duret H, Tschopp J, Moller A, Smyth M
    Cancer Res, 2012;72(22):5721-32.  2012
  200. TGFbeta signalling plays an important role in IL4-induced alternative activation of microglia.
    J Neuroinflammation, 2012;9(0):210.  2012
  201. Interleukin-33 primes mast cells for activation by IgG immune complexes.
    Authors: Kaieda S, Wang J, Shnayder R, Fishgal N, Hei H, Lee R, Stevens R, Nigrovic P
    PLoS ONE, 2012;7(10):e47252.  2012
  202. Pten loss in CD4 T cells enhances their helper function but does not lead to autoimmunity or lymphoma.
    Authors: Soond DR, Garcon F, Patton DT, Rolf J, Turner M, Scudamore C, Garden OA, Okkenhaug K
    J. Immunol., 2012;188(12):5935-43.  2012
  203. The effect of oxidized low density lipoprotein (oxLDL)-induced heme oxygenase-1 on LPS-induced inflammation in RAW 264.7 macrophage cells.
    Authors: Min KJ, Cho KH, Kwon TK
    Cell. Signal., 2012;24(6):1215-21.  2012
  204. Abcg2 deficiency augments oxidative stress and cognitive deficits in Tg-SwDI transgenic mice.
    J. Neurochem., 2012;233(0):456-469.  2012
  205. Murine bone marrow-derived macrophages differentiated with GM-CSF become foam cells by PI3Kgamma-dependent fluid-phase pinocytosis of native LDL.
    Authors: Anzinger JJ, Chang J, Xu Q, Barthwal MK, Bohnacker T, Wymann MP, Kruth HS
    J. Lipid Res., 2012;53(1):34-42.  2012
  206. Reduced VEGF production, angiogenesis, and vascular regrowth contribute to the antitumor properties of dual mTORC1/mTORC2 inhibitors.
    Authors: Falcon BL, Barr S, Gokhale PC, Chou J, Fogarty J, Depeille P, Miglarese M, Epstein DM, McDonald DM
    Cancer Res., 2011;71(5):1573-83.  2011
  207. TGF-beta limits IL-33 production and promotes the resolution of colitis through regulation of macrophage function.
    Authors: Rani R, Smulian AG, Greaves DR, Hogan SP, Herbert DR
    Eur. J. Immunol., 2011;41(7):2000-9.  2011
  208. SHIP-deficient mice develop spontaneous intestinal inflammation and arginase-dependent fibrosis.
    Authors: McLarren KW, Cole AE, Weisser SB, Voglmaier NS, Conlin VS, Jacobson K, Popescu O, Boucher JL, Sly LM
    Am. J. Pathol., 2011;179(1):180-8.  2011
  209. Intratracheal transplantation of human umbilical cord blood-derived mesenchymal stem cells attenuates Escherichia coli-induced acute lung injury in mice.
    Authors: Kim ES, Chang YS, Choi SJ, Kim JK, Yoo HS, Ahn SY, Sung DK, Kim SY, Park YR, Park WS
    Respir. Res., 2011;12(0):108.  2011
  210. Comparative kinetic analyses of gene profiles of naive CD4+ and CD8+ T cells from young and old animals reveal novel age-related alterations.
    Authors: Mirza N, Pollock K, Hoelzinger DB, Dominguez AL, Lustgarten J
    Aging Cell, 2011;10(5):853-67.  2011
  211. TNF-alpha promotes LPA1- and LPA3-mediated recruitment of leukocytes in vivo through CXCR2 ligand chemokines.
    Authors: Zhao C, Sardella A, Chun J, Poubelle PE, Fernandes MJ, Bourgoin SG
    J. Lipid Res., 2011;52(7):1307-18.  2011
  212. Immuno-therapy with anti-CTLA4 antibodies in tolerized and non-tolerized mouse tumor models.
    Authors: Persson J, Beyer I, Yumul R, Li Z, Kiem HP, Roffler S, Lieber A
    PLoS ONE, 2011;6(7):e22303.  2011
  213. Tumors Suppress In Situ Proliferation of Cytotoxic T Cells by Promoting Differentiation of Gr-1+ Conventional Dendritic Cells through IL-6.
    Authors: Diao J, Zhao J, Winter E
    J. Immunol., 2011;186(9):5058-67.  2011
  214. Involvement of adenosine A2A receptors in engulfment-dependent apoptotic cell suppression of inflammation.
    Authors: Koroskenyi K, Duro E, Pallai A, Sarang Z, Kloor D, Ucker DS, Beceiro S, Castrillo A, Chawla A, Ledent CA, Fésüs L, Szondy Z
    J. Immunol., 2011;186(12):7144-55.  2011
  215. Fine-tuning nucleophosmin in macrophage differentiation and activation.
    Authors: Guery L, Benikhlef N, Gautier T, Paul C, Jego G, Dufour E, Jacquel A, Cally R, Manoury B, Vanden Berghe T, Vandenabeele P, Droin N, Solary E
    Blood, 2011;118(17):4694-704.  2011
  216. Identification and functional characterization of novel phosphorylation sites in TAK1-binding protein (TAB) 1.
    Authors: Wolf A, Beuerlein K, Eckart C, Weiser H, Dickkopf B, Muller H, Sakurai H, Kracht M
    PLoS ONE, 2011;6(12):e29256.  2011
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    Blood, 2011;118(18):5031-9.  2011
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    Mol. Cell. Biol., 2011;31(5):971-82.  2011
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    Authors: Burton VJ, Holmes AM, Ciuclan LI
    Blood, 2011;118(17):4750-8.  2011
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FAQs

  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

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Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/24/2020
Application:

150UL SERUM WAS USED

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/12/2020
Application:

mouse tissues

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/12/2020
Application:

serum from knock-out mice

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/08/2020
Application:
Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 12/10/2019
Application:

Followed protocol exactly, 10 min exposure on film wasn't great, boosted to 20 min exposure, still not good. 5 min on digital imager and positive control spots were saturated! Much more sensitive!

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 06/06/2019
Application:

T cells isolated and stimulated with various agonists.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 04/05/2019
Application:

good!

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 03/13/2019
Application:

This array was used to measure the modulation of inflammation-associated cytokines in LPS-stimulated RAW264.7 macrophages treated with several doses of the non-digestible fraction from a corn/bean snack.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/07/2019
Application:
Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 12/26/2018
Application:
Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 12/11/2018
Application:
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 11/11/2018
Application:

High quality results with simple and straightforward protocol. No optimization needed.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 07/19/2018
Application:

instructions easy to follow. I needed a little longer than the 10 minute exposure due to low protein concentrations from my very small tissues.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 04/26/2018
Application:
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 10/20/2017
Application:
Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 09/27/2017
Application:

Very sensitive cytokine assay!

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 07/14/2017
Application:

performed on cell conditoned media from mouse fibroblasts

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 05/10/2017
Application:
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 03/15/2017
Application:

Assay tested in 200 ug of protein from pool of intestines of 10 dpf zebrafish larvae.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/27/2017
Application:

Mouse BALF samples from pooled control and treatment groups in 2 separate arrays to determine the altered cytokine response.

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 01/27/2017
Application:

Bronchoalveolar lavage fluid from C57BL/6J mouse

Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 10/06/2016
Application:
Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 09/29/2016
Application:
Serum Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006