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Proteome Profiler Mouse Cytokine Array Kit, Panel A

Catalog #: ARY006
329 Citations 33 Reviews Datasheet / COA / SDS
Contains 4 membranes - each spotted in duplicate with 40 different cytokine antibodies
Catalog # Availability Size / Price Qty
ARY006
The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample.
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Proteome Profiler Mouse Cytokine Array Kit, Panel A Summary

Kit Summary

A membrane-based antibody array for the parallel determination of the relative levels of selected mouse cytokines and chemokines. Validated for analyte detection in cell culture supernates, cell lysates, tissue lysates, serum, and plasma.

Troubleshooting Guide

Key Benefits

  • Detects 40 mouse cytokines, chemokines, and acute phase proteins simultaneously
  • Requires no specialized equipment
  • Compatible with LI-COR* and chemiluminescence detection
*If using a LI-COR, additional reagents and protocol modifications are required.  Refer to protocol.

Principle of the Assay

The Proteome Profiler Mouse Cytokine Array Kit, Panel A is a membrane-based sandwich immunoassay. Samples are mixed with a cocktail of biotinylated detection antibodies (Step 1) and then incubated with the array membrane which is spotted in duplicate with capture antibodies to specific target proteins (Step 2). Captured proteins are visualized using chemiluminescent detection reagents (Step 3). The signal produced is proportional to the amount of analyte bound.

Why Use an Antibody Array to Detect Multiple Cytokines?

Determining the expression of multiple cytokines in a single sample can be expensive, time consuming and can require specialized equipment. Performing multiple immunoprecipitations and Western blots requires time, labor, and reagents. The use of a multiplex antibody array to detect multiple cytokines in a single sample can be cost-effective and also save time and sample.

 

 

Kit Contents
  • Rectangular 4-Well Multi-dish
  • 4 Mouse Cytokine Array, Panel A nitrocellulose membranes spotted with 40 different antibodies to mouse cytokines, chemokines, and acute phase proteins
  • Array Buffer 4
  • Array Buffer 6
  • Chemi Reagent 1
  • Chemi Reagent 2
  • Detection Antibody Cocktail, Mouse Cytokine Array, Panel A
  • Streptavidin-HRP
  • Transparency Overlay Template
  • Wash Buffer Concentrate (25X)

For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.

Stability and Storage

Store the unopened kit at 2 °C to 8 °C. Do not use past kit expiration date.  

 
Simultaneously detect the levels of these cytokines, chemokines, and acute phase proteins in a single sample.
CXCL13/BLC/BCA-1 IL-5 M-CSF
C5a IL-6 CCL2/JE/MCP-1
G-CSF IL-7 CCL12/MCP-5
GM-CSF IL-10 CXCL9/MIG
CCL1/I-309 IL-12 p70 CCL3/MIP-1 alpha
CCL11/Eotaxin IL-13 CCL4/MIP-1 beta
ICAM-1 IL-16 CXCL2/MIP-2
IFN-gamma IL-17 CCL5/RANTES
IL-1 alpha/IL-1F1 IL-23 CXCL12/SDF-1
IL-1 beta/IL-1F2 IL-27 CCL17/TARC
IL-1ra/IL-1F3 CXCL10/IP-10 TIMP-1
IL-2 CXCL11/I-TAC TNF-alpha
IL-3 CXCL1/KC TREM-1
IL-4    

 

Assays for Analytes represented in the Mouse Cytokine Array Kit, Panel A

Analyte Quantikine® ELISA Kits DuoSet® ELISA Development Systems
CXCL13/BLC/BCA-1 MCX130 DY470
C5a   DY2150
G-CSF MCS00 DY414
GM-CSF MGM00 DY415
CCL1/I-309/TCA-3   DY845
CCL11/Eotaxin MME00 DY420
ICAM-1 MIC100 DY796
IFN-gamma MIF00 DY485
IL-1 alpha MLA00 DY400
IL-1 beta MLB00B DY401
IL-1ra MRA00 DY480
IL-2 M2000 DY402
IL-3 M3000 DY403
IL-4 M4000B DY404
IL-5 M5000 DY405
IL-6 M6000B DY406
IL-7 M7000 DY407
IL-10 M1000b DY417
IL-12 p70 M1270 DY419
IL-13 M1300CB DY413
IL-16    
IL-17 M1700 DY421
IL-23 M2300 DY1887
IL-27 M2728  
CXCL10/IP-10/CRG-2 MCX100 DY466
CXCL11/I-TAC   DY572
CXCL1/KC MKC00B DY453
M-CSF MMC00 DY416
CCL2/MCP-1/JE MJE00 DY479
CCL12/MCP-5 MCC120 DY428
CXCL9/MIG MCX900 DY492
CCL3/MIP-1 alpha MMA00 DY450
CCL4/MIP-1 beta MMB00 DY451
CXCL2/MIP-2 MM200 DY452
CCL5/RANTES MMR00 DY478
CXCL12/SDF-1 MCX120 DY460
CCL17/TARC   DY529
TIMP-1 MTM100 DY980
TNF-alpha MTA00 DY410
TREM-1 MTRM10 DY1187

Specifications

Shipping Conditions
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Species
Mouse

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Scientific Data

The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. View Larger

The Mouse Cytokine Array, Panel A utilizes capture antibodies spotted onto a nitrocellulose membrane to allow high-throughput multi-analyte profiling of 40 cytokines, chemokines, and more in a single sample. Mouse splenocytes treated with 100 ng/mL LPS for 20 hours were mixed with a cocktail of biotinylated detection antibodies, and then incubated with the Mouse Cytokine Array. The array was then incubated with streptavidin-horseradish peroxidase followed by chemiluminescent detection. After detection, the array data were quantitated to generate a protein profile (histogram). The table shows the analytes detected and their location on the membrane.

The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. View Larger

The Mouse Cytokine Array detects multiple analytes in cell culture lysates and supernates. (A. and B.) Lysates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µg of lysate was used for each array shown. (C. and D.) Supernates of RAW264.7 mouse monocyte/macrophage cell line untreated or treated with 100 ng/mL LPS for 24 hours. 100 µL of supernate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates. View Larger

The Mouse Cytokine Array detects multiple analytes in mouse serum and tissue lysates. (A and B.) Samples of mouse serum from two different vendors. 200 µL of serum was used for each array shown. (C. and D.) Lysates of mouse lung tissue and mouse stomach tissue. 200 µg of lysate was used for each array shown. Array images were collected and analyzed using the LI-COR Odyssey Infrared Imaging System.

Citations for Proteome Profiler Mouse Cytokine Array Kit, Panel A

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

329 Citations: Showing 1 - 10
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  1. Sulfaphenazole reduces thermal and�pressure injury severity through rapid restoration of tissue perfusion
    Authors: CT Turner, M Pawluk, J Bolsoni, MR Zeglinski, Y Shen, H Zhao, T Ponomarev, KC Richardson, CR West, A Papp, DJ Granville
    Scientific Reports, 2022;12(1):12622.  2022
  2. Presenilin 2 N141I mutation induces hyperactive immune response through the epigenetic repression of REV-ERBalpha
    Authors: H Nam, Y Lee, B Kim, JW Lee, S Hwang, HK An, KM Chung, Y Park, J Hong, K Kim, EK Kim, HK Choe, SW Yu
    Nature Communications, 2022;13(1):1972.  2022
  3. SH005S7 Overcomes Primary and Acquired Resistance of Non-Small Cell Lung Cancer by Combined MET/EGFR/HER3 Inhibition
    Authors: S Kang, JH Kim, J Hong, JH Moon, YY Kwon, SG Ko
    BioMed Research International, 2022;2022(0):1840541.  2022
  4. Systemic checkpoint blockade by PD-L1 single-chain antibody confers potent anti-tumor immunity and long-term survival
    Authors: H Wang, V Khattar, JA Hensel, R Ashton, Y Lu, AG Sorace, Y Wang, JS Deshane, JL Mieher, C Deivanayag, S Ponnazhaga
    Molecular Cancer Therapeutics, 2022;0(0):.  2022
  5. Mechanisms Underlying Antipsychotic-Induced NAFLD and Iron Dysregulation: A Multi-Omic Approach
    Authors: M May, D Barlow, R Ibrahim, KL Houseknech
    Oncogene, 2022;10(6):.  2022
  6. Paclitaxel binds and activates C5aR1: A new potential therapeutic target for the prevention of chemotherapy-induced peripheral neuropathy and hypersensitivity reactions
    Authors: L Brandolini, M d'Angelo, R Novelli, V Castelli, C Giorgio, A Sirico, P Cocchiaro, F D'Egidio, E Benedetti, C Cristiano, A Bugatti, A Ruocco, PG Amendola, C Talarico, C Manelfi, D Iaconis, A Beccari, AU Quadros, TM Cunha, A Caruso, R Russo, A Cimini, A Aramini, M Allegretti
    Cell Death & Disease, 2022;13(5):500.  2022
  7. Naegleria fowleri Cathepsin B Induces a Pro-Inflammatory Immune Response in BV-2 Microglial Cells via NF-kappaB and AP-1 Dependent-MAPK Signaling Pathway
    Authors: HG Lê, JM Kang, TC Võ, BK Na
    International Journal of Molecular Sciences, 2022;23(15):.  2022
  8. Spontaneous Myocarditis in Mice Predisposed to Autoimmune Disease: Including Vaccination-Induced Onset
    Authors: T Hayashi, M Ichikawa, I Konishi
    Biomedicines, 2022;10(6):.  2022
  9. Macrophage-Specific, Mafb-Deficient Mice Showed Delayed Skin Wound Healing
    Authors: Y Inoue, CW Liao, Y Tsunakawa, IL Tsai, S Takahashi, M Hamada
    International Journal of Molecular Sciences, 2022;23(16):.  2022
  10. Brain motor and fear circuits regulate leukocytes during acute stress
    Authors: WC Poller, J Downey, AA Mooslechne, N Khan, L Li, CT Chan, CS McAlpine, C Xu, F Kahles, S He, H Janssen, JE Mindur, S Singh, MG Kiss, L Alonso-Her, Y Iwamoto, RH Kohler, LP Wong, K Chetal, SJ Russo, RI Sadreyev, R Weissleder, M Nahrendorf, PS Frenette, M Divangahi, FK Swirski
    Nature, 2022;0(0):.  2022
  11. Synthetic Essentiality of Tryptophan 2,3-dioxygenase 2 in APC-Mutated Colorectal Cancer
    Authors: R Lee, J Li, J Li, CJ Wu, S Jiang, WH Hsu, D Chakravart, P Chen, KA LaBella, J Li, DJ Spring, D Zhao, YA Wang, RA DePinho
    Cancer Discovery, 2022;0(0):.  2022
  12. Maternal soybean diet on prevention of obesity-related breast cancer through early-life gut microbiome and epigenetic regulation: Maternal soybean prevention on obesity-related breast cancer
    Authors: M Chen, S Li, I Arora, N Yi, M Sharma, Z Li, TO Tollefsbol, Y Li
    The Journal of nutritional biochemistry, 2022;0(0):109119.  2022
  13. BEX1 is a critical determinant of viral myocarditis
    Authors: CR Martens, LE Dorn, AD Kenney, SS Bansal, JS Yount, F Accornero
    PloS Pathogens, 2022;18(2):e1010342.  2022
  14. NIL10: A New IL10-Receptor Binding Nanoparticle That Induces Cardiac Protection in Mice and Pigs Subjected to Acute Myocardial Infarction through STAT3/NF-kappaB Activation
    Authors: L Tesoro, I Hernández, R Ramírez-Ca, J Díez-Mata, N Alcharani, B Jiménez-Gu, K Ovejero-Pa, M Filice, JL Zamorano, M Saura, C Zaragoza, L Botana
    Pharmaceutics, 2022;14(10):.  2022
  15. Survival of stem cells and progenitors in the intestine is regulated by LPA5-dependent signaling
    Authors: Z Liang, P He, Y Han, CC Yun
    Cellular and Molecular Gastroenterology and Hepatology, 2022;0(0):.  2022
  16. P62/SQSTM1 enhances osteogenesis and attenuates inflammatory signals in bone marrow microenvironment
    Authors: D Agas, V Gabai, AA Sufianov, A Shneider, M Giovanna S
    General and comparative endocrinology, 2022;320(0):114009.  2022
  17. Mesenchymal stem cells empower T cells in the lymph nodes via MCP-1/PD-L1 axis
    Authors: Y He, Y Qu, B Meng, W Huang, J Tang, R Wang, Z Chen, X Kou, S Shi
    Cell Death & Disease, 2022;13(4):365.  2022
  18. Inhibiting MNK kinases promotes macrophage immunosuppressive phenotype to limit CD8+ T cell anti-tumor immunity
    Authors: TN Pham, C Spaulding, MA Shields, AE Metropulos, DN Shah, MG Khalafalla, DR Principe, DJ Bentrem, HG Munshi
    JCI Insight, 2022;0(0):.  2022
  19. Recombinant Klotho Protein Ameliorates Myocardial Ischemia/Reperfusion Injury by Attenuating Sterile Inflammation
    Authors: J Myung, JH Beom, JH Kim, JS Woo, I Park, SP Chung, YE Chung, JS You
    Biomedicines, 2022;10(4):.  2022
  20. Elevated expression of the colony-stimulating factor 1 (CSF1) induces prostatic intraepithelial neoplasia dependent of epithelial-Gp130
    Authors: OJ Kwon, B Zhang, D Jia, L Zhang, X Wei, Z Zhou, D Liu, KT Huynh, K Zhang, Y Zhang, P Labhart, A Sboner, C Barbieri, MC Haffner, CJ Creighton, L Xin
    Oncogene, 2022;0(0):.  2022
  21. Social isolation induces neuroinflammation and microglia overactivation, while dihydromyricetin prevents and improves them
    Authors: AJ Al Omran, AS Shao, S Watanabe, Z Zhang, J Zhang, C Xue, J Watanabe, DL Davies, XM Shao, J Liang
    Journal of Neuroinflammation, 2022;19(1):2.  2022
  22. The downregulation of type I IFN signaling in G-MDSCs under tumor conditions promotes their development towards an immunosuppressive phenotype
    Authors: Y Sun, X Han, C Shang, Y Wang, B Xu, S Jiang, Y Mo, D Wang, Y Ke, X Zeng
    Cell Death & Disease, 2022;13(1):36.  2022
  23. Apiaceous vegetables protect against acrolein-induced pulmonary injuries through modulating hepatic detoxification and inflammation in C57BL/6 male mice
    Authors: MC Redding, JH Pan, YJ Kim, M Batish, J Trabulsi, JH Lee, JK Kim
    The Journal of nutritional biochemistry, 2022;101(0):108939.  2022
  24. TLR4-Mediated Inflammatory Responses Regulate Exercise-Induced Molecular Adaptations in Mouse Skeletal Muscle
    Authors: H Fujiyoshi, T Egawa, E Kurogi, T Yokokawa, K Kido, T Hayashi
    International Journal of Molecular Sciences, 2022;23(3):.  2022
  25. Modulation of the cell membrane lipid milieu by peroxisomal beta-oxidation induces Rho1 signaling to trigger inflammatory responses
    Authors: AS Nath, BD Parsons, S Makdissi, RL Chilvers, Y Mu, CM Weaver, I Euodia, KA Fitze, J Long, M Scur, DP Mackenzie, AP Makrigiann, N Pichaud, LH Boudreau, AJ Simmonds, CA Webber, B Derfalvi, Y Hammon, RA Rachubinsk, F Di Cara
    Cell Reports, 2022;38(9):110433.  2022
  26. Oral administration of human carbonic anhydrase I suppresses colitis in a murine inflammatory bowel disease model
    Authors: K Tange, S Yagi, E Takeshita, M Abe, Y Yamamoto, H Tomida, T Kawamura, M Hanayama, B Matsuura, Y Ikeda, Y Hiasa
    Scientific Reports, 2022;12(1):17983.  2022
  27. PEGylation of Metal Oxide Nanoparticles Modulates Neutrophil Extracellular Trap Formation
    Authors: HT Snoderly, KA Freshwater, C Martinez d, DM Panchal, JN Vito, MF Bennewitz
    Biosensors, 2022;12(2):.  2022
  28. Differential progression of unhealthy diet-induced hepatocellular carcinoma in obese and non-obese mice
    Authors: E Hymel, E Vlock, KW Fisher, PA Farazi
    PLoS ONE, 2022;17(8):e0272623.  2022
  29. Neuronal deletion of MnSOD in mice leads to demyelination, inflammation and progressive paralysis that mimics phenotypes associated with progressive multiple sclerosis
    Authors: S Bhaskaran, G Kumar, N Thadathil, KM Piekarz, S Mohammed, SD Lopez, R Qaisar, D Walton, JL Brown, A Murphy, N Smith, D Saunders, MJ Beckstead, S Plafker, TL Lewis, R Towner, SS Deepa, A Richardson, RC Axtell, H Van Remmen
    Redox Biology, 2022;59(0):102550.  2022
  30. CD8+ T-cell responses are boosted by dual PD-1/VEGFR2 blockade after EGFR inhibition in Egfr-mutant lung cancer
    Authors: K Nishii, K Ohashi, S Tomida, T Nakasuka, A Hirabae, S Okawa, J Nishimura, H Higo, H Watanabe, H Kano, C Ando, G Makimoto, K Ninomiya, Y Kato, T Kubo, E Ichihara, K Hotta, M Tabata, S Toyooka, H Udono, Y Maeda, K Kiura
    Cancer Immunology Research, 2022;0(0):.  2022
  31. Functional Complementation of Anti-Adipogenic Phytonutrients for Obesity Prevention and Management
    Authors: Y Urasaki, TT Le
    Nutrients, 2022;14(20):.  2022
  32. Intermittent scavenging of storage lesion from stored red blood cells by electrospun nanofibrous sheets enhances their quality and shelf-life
    Authors: S Pandey, M Mahato, P Srinath, U Bhutani, TJ Goap, P Ravipati, PK Vemula
    Nature Communications, 2022;13(1):7394.  2022
  33. FOXP3+ macrophage represses acute ischemic stroke-induced neural inflammation
    Authors: W Cai, M Hu, C Li, R Wu, D Lu, C Xie, W Zhang, T Li, S Shen, H Huang, W Qiu, Q Liu, Y Lu, Z Lu
    Autophagy, 2022;0(0):1-20.  2022
  34. Bio-inspired poly-DL-serine materials resist the foreign-body response
    Authors: D Zhang, Q Chen, Y Bi, H Zhang, M Chen, J Wan, C Shi, W Zhang, J Zhang, Z Qiao, J Li, S Chen, R Liu
    Nature Communications, 2021;12(1):5327.  2021
  35. Signaling through Syk or CARD9 Mediates Species-Specific Anti-Candida Protection in Bone Marrow Chimeric Mice
    Authors: E Zajta, K Csonka, A Tóth, L Tiszlavicz, T Németh, A Orosz, Á Novák, M Csikós, C Vágvölgyi, A Mócsai, A Gácser
    MBio, 2021;12(4):e0160821.  2021
  36. The Protective Effect of Anthocyanins Extracted from Aronia Melanocarpa Berry in Renal Ischemia-Reperfusion Injury in Mice
    Authors: L Li, J Li, H Xu, F Zhu, Z Li, H Lu, J Zhang, Z Yang, Y Liu
    Mediators of Inflammation, 2021;2021(0):7372893.  2021
  37. Knockout of the KH-Type Splicing Regulatory Protein Drives Glomerulonephritis in MRL-Faslpr Mice
    Authors: L Schmidtke, M Meineck, S Saurin, S Otten, F Gather, K Schrick, R Käfer, W Roth, H Kleinert, J Weinmann-M, A Pautz
    Cells, 2021;10(11):.  2021
  38. Differential expression of Triggering Receptor Expressed on Myeloid cells 2 (Trem2) in tissue eosinophils
    Authors: AC Sek, CM Percopo, AK Boddapati, M Ma, WE Geslewitz, JO Krumholz, JB Lack, HF Rosenberg
    Journal of leukocyte biology, 2021;0(0):.  2021
  39. Sulfated polysaccharide directs therapeutic angiogenesis via endogenous VEGF secretion of macrophages
    Authors: Y Yu, K Dai, Z Gao, W Tang, T Shen, Y Yuan, J Wang, C Liu
    Science Advances, 2021;7(7):.  2021
  40. Proteoglycan-4 is an essential regulator of synovial macrophage polarization and inflammatory macrophage joint infiltration
    Authors: M Qadri, GD Jay, LX Zhang, TA Schmidt, J Totonchy, KA Elsaid
    Arthritis Research & Therapy, 2021;23(1):241.  2021
  41. Social Isolation Induces Neuroinflammation And Microglia Overactivation, While Dihydromyricetin Prevents And Improves Them
    Authors: AJA Omran, AS Shao, S Watanabe, Z Zhang, J Zhang, C Xue, J Watanabe, DL Davies, XM Shao, J Liang
    Research square, 2021;0(0):.  2021
  42. Tumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
    Authors: LG Lima, S Ham, H Shin, EPZ Chai, ESH Lek, RJ Lobb, AF Müller, S Mathivanan, B Yeo, Y Choi, BS Parker, A Möller
    Nature Communications, 2021;12(1):3543.  2021
  43. Complement-containing small extracellular vesicles from adventitial fibroblasts induce pro-inflammatory and metabolic reprogramming in macrophages
    Authors: S Kumar, MG Frid, H Zhang, M Li, S Riddle, RD Brown, SC Yadav, MK Roy, ME Dzieciatko, A D'Alessand, KC Hansen, KR Stenmark
    JCI Insight, 2021;0(0):.  2021
  44. JAK/STAT inhibitor therapy partially rescues the lipodystrophic autoimmune phenotype in Clec16a KO mice
    Authors: R Pandey, M Bakay, BP Strenkowsk, HS Hain, H Hakonarson
    Scientific Reports, 2021;11(1):7372.  2021
  45. Moderately pathogenic maternal influenza A virus infection disrupts placental integrity but spares the fetal brain
    Authors: AM Antonson, AD Kenney, HJ Chen, KN Corps, JS Yount, TL Gur
    Brain, Behavior, and Immunity, 2021;0(0):.  2021
  46. Impact of controlled high-sucrose and high-fat diets on eosinophil recruitment and cytokine content in allergen-challenged mice
    Authors: CM Percopo, M McCullough, AR Limkar, KM Druey, HF Rosenberg
    PLoS ONE, 2021;16(8):e0255997.  2021
  47. Microglia-derived interleukin-10 accelerates post-intracerebral hemorrhage hematoma clearance by regulating CD36
    Authors: Q Li, X Lan, X Han, F Durham, J Wan, A Weiland, RC Koehler, J Wang
    Brain, Behavior, and Immunity, 2021;94(0):437-457.  2021
  48. Macrophage-mediated RON signaling supports breast cancer growth and progression through modulation of IL-35
    Authors: SJ Ruiz-Torre, JR Bourn, NM Benight, BG Hunt, C Lester, SE Waltz
    Oncogene, 2021;0(0):.  2021
  49. CD4+ effector T cells accelerate Alzheimer's disease in mice
    Authors: J Machhi, P Yeapuri, Y Lu, E Foster, R Chikhale, J Herskovitz, KL Namminga, KE Olson, MM Abdelmoaty, J Gao, RM Quadros, T Kiyota, L Jingjing, BD Kevadiya, X Wang, Y Liu, LY Poluektova, CB Gurumurthy, RL Mosley, HE Gendelman
    Journal of Neuroinflammation, 2021;18(1):272.  2021
  50. KCNK3 Mutation Causes Altered Immune Function in Pulmonary Arterial Hypertension Patients and Mouse Models
    Authors: JD West, ED Austin, EM Rizzi, L Yan, H Tanjore, AL Crabtree, CS Moore, G Muthian, EJ Carrier, DA Jacobson, R Hamid, PL Kendall, S Majka, A Rathinasab
    International Journal of Molecular Sciences, 2021;22(9):.  2021
  51. Reduction of Cell Proliferation by Acute C2H6O Exposure
    Authors: S Baldari, I Manni, G Di Rocco, F Paolini, B Palermo, G Piaggio, G Toietta
    Cancers, 2021;13(19):.  2021
  52. Sustained oxygenation accelerates diabetic wound healing by promoting epithelialization and angiogenesis and decreasing inflammation
    Authors: Y Guan, H Niu, Z Liu, Y Dang, J Shen, M Zayed, L Ma, J Guan
    Science Advances, 2021;7(35):.  2021
  53. Humidifier disinfectant, sodium dichloroisocyanurate (NaDCC): assessment of respiratory effects to protect workers' health
    Authors: D Seo, J Jo
    Scientific Reports, 2021;11(1):15681.  2021
  54. Pneumococcal Extracellular Vesicles Modulate Host Immunity
    Authors: SS Yerneni, S Werner, JH Azambuja, N Ludwig, R Eutsey, SD Aggarwal, PC Lucas, N Bailey, TL Whiteside, PG Campbell, NL Hiller
    MBio, 2021;0(0):e0165721.  2021
  55. Three-dimensional culture method enhances the therapeutic efficacies of tonsil-derived mesenchymal stem cells in murine chronic colitis model
    Authors: EM Song, YH Joo, AR Choe, Y Park, CH Tae, JT Hong, CM Moon, SE Kim, HK Jung, KN Shim, KA Cho, I Jo, SA Jung
    Scientific Reports, 2021;11(1):19589.  2021
  56. Distinct transcription factor networks control neutrophil-driven inflammation
    Authors: TE Khoyratty, Z Ai, I Ballestero, HL Eames, S Mathie, S Martín-Sal, L Wang, A Hemmings, N Willemsen, V von Werz, A Zehrer, B Walzog, E van Grinsv, A Hidalgo, IA Udalova
    Nature Immunology, 2021;0(0):.  2021
  57. Polymerized albumin restores impaired hemodynamics in endotoxemia and polymicrobial sepsis
    Authors: DA Belcher, AT Williams, AF Palmer, P Cabrales
    Scientific Reports, 2021;11(1):10834.  2021
  58. Recombinant annexin A2 inhibits peripheral leukocyte activation and brain infiltration after traumatic brain injury
    Authors: N Liu, J Han, Y Li, Y Jiang, SX Shi, J Lok, M Whalen, AS Dumont, X Wang
    Journal of Neuroinflammation, 2021;18(1):173.  2021
  59. Chamaecyparis obtusa (Siebold & Zucc.) Endl. leaf extracts prevent inflammatory responses via inhibition of the JAK/STAT axis in RAW264.7 cells
    Authors: YJ Kwon, EB Seo, SK Kim, KH Noh, H Lee, YW Joung, HM Shin, YA Jang, YM Kim, JT Lee, SK Ye
    Journal of ethnopharmacology, 2021;0(0):114493.  2021
  60. Vaccine delivery alerts innate immune systems for more immunogenic vaccination
    Authors: Z Li, Y Cao, Y Li, Y Zhao, X Chen
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  1. Will the Array identification number stamped on the Array membrane interfere with detection if it is not cut-off before the membrane is blocked?

    • The dye used for printing the Array identification number on the membranes will fluoresce and interfere with the LI-COR detection. It is critical that the number is cut off before beginning the experiment. 

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Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Andres Ocampo on 10/11/2022

Mouse Cytokine Antibody Array was used in serum from Prolactin Receptor wild type baby mice after the mother's high-fat diet feeding for 21 days of lactation, and results were compared with Control Diet (CD). A low-level pro-inflammatory mechanism could be confirmed due to the modulation of inflammation-associated cytokines.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 03/01/2022
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Martin Torres on 10/01/2021

The array was used to test the anti-inflammatory potential from Octopus vulgaris ink extracts on LPS-stimulated RAW 264.7 cells. Results obtained were in agreement with other biochemical and in vitro test. We have used this kit in several LPS-challenged murine macrophages. Fig. A shows the expression level of each cytokine against the control (LPS only-treated macrophages); B shows a bioinformatic arrangement of the modulated cytokines, and C shows a predictive analysis from the results obtained in this kit.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Martin Torres on 09/23/2021

Pro and anti-inflammatory cytokines were evaluated in RAW264.7 macrophages treated with LPS (1 microgram/mL) and a South American berry (ABJ). Results obtained showed the modulation of several cytokines indicated in this kit. We have conducted several evaluations of these cytokines using this kit and results never disappoint. What we like the most is that this assay allows the evaluation of several cytokines that would take a lot of time by conventional techniques such as ELISA or Western Blot.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 09/23/2021

20 ul serum was used to perform the experiment.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 08/31/2021
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anil Singh on 08/25/2021

mouse serum 200ul used for cancer model

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Gaurav Kumar on 08/17/2021

Used serum to see the role of gene in pavreatic cancer

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Allison Scarpitti on 05/10/2021

Phenomenal results, consistent performance and excellent translation to use with Streptavidin-800. On step 11, I swapped the Streptavidin-HRP for a 1:2,000 ratio of Streptavidin-800. Then followed with the wash steps and the result is as posted.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 03/06/2021
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By jinxiu PAN on 02/24/2020

150UL SERUM WAS USED

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/12/2020

mouse tissues

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/12/2020

serum from knock-out mice

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/08/2020
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 12/10/2019

Followed protocol exactly, 10 min exposure on film wasn't great, boosted to 20 min exposure, still not good. 5 min on digital imager and positive control spots were saturated! Much more sensitive!

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 06/06/2019

T cells isolated and stimulated with various agonists.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 04/05/2019

good!

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Ivan Luzardo on 03/13/2019

This array was used to measure the modulation of inflammation-associated cytokines in LPS-stimulated RAW264.7 macrophages treated with several doses of the non-digestible fraction from a corn/bean snack.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Brian Englar on 02/07/2019
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 12/26/2018
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Ivan Luzardo on 12/11/2018
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 11/11/2018

High quality results with simple and straightforward protocol. No optimization needed.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 07/19/2018

instructions easy to follow. I needed a little longer than the 10 minute exposure due to low protein concentrations from my very small tissues.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 04/26/2018
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Adam Guess on 10/20/2017
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 09/27/2017

Very sensitive cytokine assay!

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 07/14/2017

performed on cell conditoned media from mouse fibroblasts

Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Michelle Chen on 05/10/2017
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Camila Idelí Morales Fénero on 03/15/2017

Assay tested in 200 ug of protein from pool of intestines of 10 dpf zebrafish larvae.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 02/27/2017

Mouse BALF samples from pooled control and treatment groups in 2 separate arrays to determine the altered cytokine response.

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 01/27/2017

Bronchoalveolar lavage fluid from C57BL/6J mouse

Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Anonymous on 10/06/2016
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006
Proteome Profiler Mouse Cytokine Array Kit, Panel A
By Kihong Lim on 09/29/2016
Proteome Profiler Mouse Cytokine Array Kit, Panel A ARY006