SARS-CoV-2 LAMP primers (N gene)

Biological Activity

SARS-CoV-2 LAMP primers (N gene) contain a premixed formulation of B3, F3, FIP, BIP, LB and LF clinically validated primers targeting the N gene of SARS-CoV-2. Supplied at 10x concentration in nuclease free water. Can be used directly in reverse transcription loop-mediated isothermal amplification (RT-LAMP) assays for detection of SARS-CoV-2 as described by Zhang et al (2020) and Buck et al (2020). Sufficient quantity for 200 x 25 μL RT-LAMP reactions. No need to resuspend and mix.

Technical Data

The technical data provided above is for guidance only. For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.

Background References

1. Standard operating procedures for SARS-CoV-2 detection by a clinical diagnostic RT-LAMP assay.
   Buck et al.
   Medrxiv - Paper not yet peer reviewed, 2020;
2. Rapid molecular detection of SARS-CoV-2 (COVID-19) virus RNA using colorimetric LAMP.
   Zhang et al.
   Medrxiv - Paper not yet peer reviewed, 2020;
3. A colorimetric RT-LAMP assay and LAMP-sequencing for detecting SARS-CoV-2 RNA in clinical samples.
   Loan Dao Thi et al.
   Sci.Transl.Med., 2020;12:eabc7075

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